Table 3.. Characterization of GeneChip small sample target labeling assay

  Cell source  
   Total RNA used initially  
   cRNA yield, µg  
   % of "present" call  
   3'/5' ratio, GAPDH  
  N CD4     500 ng     54.2     44.4     2.13  
  N CD4     500 ng     53.4     47.7     2.84  
  N CD4*     5 µg     52.2     46.7     1.83  
  N CD4*     5 µg     54.3     44.9     1.44  
  Pool-AA1-1     500 ng     51.8     45.4     2.17  
  Pool-AA1-2     500 ng     49.5     46.6     2.68  
  Pool-AA1-3     500 ng     50.6     44.1     2.67  
  Pool-AA2     500 ng     50.6     48.5     3.27  
  Pool-N1-1     500 ng     53.3     41.9     2.55  
  Pool-N1-2     500 ng     51.7     44.7     1.92  
  Pool-N1-3     500 ng     54.8     47.0     1.94  
  Pool-N2  
   500 ng  
   48.9  
   46.7  
   2.80  

GAPDH indicates glyceraldehyde-3-phosphate dehydrogenase; N, healthy donor.

* Standard protocol.