Blood, 1955, Vol. 10, No. 10, pp. 979-986.
© 1955 American Society of Hematology, Inc.
Studies on Human Myoglobin
I. Myoglobin in Sickle Cell Disease
KARL SINGER 1,
BASIL ANGELOPOULOS 1, and
BRACHA RAMOT 1
1 Department of Hematologic Research, Medical Research Institute, Michael
Reese Hospital, Chicago, Illinois.
The problem whether patients with sickle cell anemia have not only a pathologic (type S) hemoglobin, but also an abnormal myoglobin, was investigated by
means of spectroscopic and electrophoretic methods. Purified, hemoglobin-free
met-myoglobin preparations, obtained from normal adults and from individuals
with sickle cell anemia, were compared. No differences could be detected with
either technic.
The electrophoretic studies revealed that adult met-myoglobin moves faster
in an acid than in an alkaline buffer. Regardless of the buffer used, adult met-myoglobin moves always slower than either met-hemoglobin A or S. The met-compounds of hemoglobin A and S move faster than their respective oxy-compounds in an acid medium, but slower in an alkaline medium.
Submitted on May 24, 1955
Accepted on June 12, 1955
