Blood, 1955, Vol. 10, No. 12, pp. 1183-1203.
© 1955 American Society of Hematology, Inc.
A Study of the Morphology of the Living Cells of Blood
and Bone Marrow in Supravital Films with the
Phase Contrast Microscope
II. Blood and Bone Marrow from Various Hematologic Dyscrasias
G. ADOLPH ACKERMAN 1 and
NICHOLAS C. BELLIOS 1
1 Department of Anatomy, Ohio State University College of Medicine, Columbus, Ohio.
The cells of the blood and bone marrow from various blood dyscrasias have
been studied in the living state and compared with normal cells of the same
lineage by means of vital films and phase contrast and bright field microscopes.
The precise morphology of the cells of the blood and bone marrow in a normal
and diseased condition is most accurately obtained by an examination of the
cells in a living condition. Such studies are possible with supravital staining and
the phase contrast microscope. Since cellular structure and function are interdependent an alteration in one is necessarily reflected in the other. Further insight into the chemical structure and composition of cells of the blood and bone
marrow in normal and diseased conditions may be obtained from cytochemical
investigations. However, it is essential that cytochemical localization of substances within the cell must have its foundation in the morphology of the intact
living cell.
Submitted on March 8, 1954
Accepted on August 27, 1955
