Blood, 1955, Vol. 10, No. 12, pp. 1228-1235.
© 1955 American Society of Hematology, Inc.
An In Vitro Study of Antileukemic Serum
JOHN S. THOMPSON 1
1 Department of Medicine and the Argonne Cancer Research Hospital, The
University of Chicago.
1. A rabbit anti-DBA2-mouse-induced lymphogenous leukemia serum was
prepared that contained antibodies to normal lymphocytic and to leukemic
lymphocytic antigens, as determined by the complement fixation test of Thornton and his associates.
2. When this antiserum was incubated with normal lymphocytic antigen, all
of its complement fixing activity was removed except that which reacted with
the leukemic tissue. It appears that an antibody or group of antibodies was
produced which was specific for this leukemia.
3. An antiserum to lymphogenous leukemia, induced in DBA2 mice, given
prophylactically or therapeutically, did not significantly protect other DBA2
mice that had been inoculated with a transplantable leukemia of the same cell
type.
4. The failure to induce leukemia in young, DBA2 female mice by painting
them with 20-methylcholanthrene in benzene is reported.
Submitted on April 18, 1955
Accepted on August 27, 1955
