|
|||||||||
|
|
|
|
|
|
|
|
|
|
|
1 University of Minnesota Hospital Laboratories, Minneapolis, Minn.
A simple method for staining non-hemoglobin iron in erythrocytes, normoblasts, macrophages, and other cells containing particulate iron in new or old
films of cellular fluids or imprints of tissues and organs is presented. This method
consists in using the prussian blue reagent as a type of counterstain; no separate
decolorization is necessary. The preparations obtained resemble the original
preparations except that iron stands out as a vivid blue-green material. The method is particularly useful in studying conditions accompanied by
varying degrees of iron excess or hemosiderosis of the marrow. The stainable iron is all virtually the same color. The diffuse blue-green color
of the cytoplasm of macrophages might be attributed to the more soluble form
of iron, ferritin. Stainable iron is visible in normoblasts and erythrocytes as well as in macrophages in sections subjected to the prussian blue reaction. A prussian blue method using formalin fixation on fresh films of marrow7 has
also been shown to be useful in the demonstration of particulate iron in previously stained films of marrow and blood. The formalin fixation appears to
bring about a higher percentage of siderocytes.
This article has been cited by other articles:
| |||||||||||
 |
 |
 |
|||||||
 |
 |
 |
 |
 |
 |
 |
 |
||
| Copyright © 1955 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
