|
|||||||||
|
|
|
|
|
|
|
|
|
|
|
1 Department of Medicine, Harvard Medical School, the Medical Clinic, Peter
Bent Brigham Hospital, and the Children’s Cancer Research Foundation, Children’s
Medical Center, Boston.
A method has been described whereby erythropoiesis may be studied in vitro
by measuring simultaneously oxygen consumption and the rate of incorporation
of radioactive glycine into hemin by rabbit bone marrow. This rate shows only
a very slight decrease in the first 20 hours. The optimal pH is about 8.0. The
effect of normal serum on this process has been studied. Duplicate determinations
show good agreement, but various normal sera show considerable variation in
the ability to promote hemin synthesis. Normal serum has been shown to have a
marked stimulating effect on hemin synthesis even after considerable dilution.
Human serum halts completely the synthesis of hemin by rabbit bone marrow.
An interesting dissociations between hemin synthesis and oxygen consumption
has been pointed out. This effect is most apparent in experiments on heat-deproteinized serum. This material retains its ability to stimulate oxygen consumption
but does not stimulate hemin synthesis at all. The relation of the stimulating
effect of normal serum to the hypothetical erythropoietic hormone is discussed.
This article has been cited by other articles:
| |||||||||||
 |
 |
 |
|||||||
 |
 |
 |
 |
 |
 |
 |
 |
||
| Copyright © 1955 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
