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Blood, 1 July 2002, Vol. 100, No. 1, pp. 107-119
HEMATOPOIESIS
Novel transcription factors in human CD34 antigen-positive
hematopoietic cells
Ignatius Gomes,
Tiffany T. Sharma,
Seby Edassery,
Noreen Fulton,
Brenton G. Mar, and
Carol A. Westbrook
From the Department of Medicine, Section of Hematology
and Oncology, University of Illinois at Chicago.
Transcription factors (TFs) and the regulatory proteins that
control them play key roles in hematopoiesis, controlling basic processes of cell growth and differentiation; disruption of these processes may lead to leukemogenesis. Here we attempt to identify functionally novel and partially characterized TFs/regulatory proteins
that are expressed in undifferentiated hematopoietic tissue. We
surveyed our database of 15 970 genes/expressed sequence tags
(ESTs) representing the normal human CD34+ cells
transcriptosome (http://westsun.hema.uic.edu/cd34.html), using the
UniGene annotation text descriptor, to identify genes with motifs
consistent with transcriptional regulators; 285 genes were identified.
We also extracted the human homologues of the TFs reported in the
murine stem cell database (SCdb; http://stemcell.princeton.edu/), selecting an additional 45 genes/ESTs. An exhaustive literature search
of each of these 330 unique genes was performed to determine if
any had been previously reported and to obtain additional
characterizing information. Of the resulting gene list, 106 were
considered to be potential TFs. Overall, the transcriptional regulator
dataset consists of 165 novel or poorly characterized genes, including 25 that appeared to be TFs. Among these novel and poorly characterized genes are a cell growth regulatory with ring finger domain protein (CGR19, Hs.59106), an RB-associated CRAB repressor
(RBAK, Hs.7222), a death-associated transcription factor 1 (DATF1, Hs.155313), and a p38-interacting protein
(P38IP, Hs. 171185). The identification of these
novel and partially characterized potential transcriptional regulators
adds a wealth of information to understanding the molecular aspects of
hematopoiesis and hematopoietic disorders.
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