SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Brummel, K. E. Articles by Mann, K. G. Search for Related Content PubMed PubMed Citation Articles by Brummel, K. E. Articles by Mann, K. G. Related Collections Hemostasis, Thrombosis, and Vascular Biology Related Letter in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 July 2002, Vol. 100, No. 1, pp. 148-152 HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Thrombin functions during tissue factor-induced blood coagulation Kathleen E. Brummel, Sara G. Paradis, Saulius Butenas, and Kenneth G. Mann From the Department of Biochemistry, University of Vermont, College of Medicine, Burlington, VT. Tissue factor-induced blood coagulation was studied in 20 individuals, for varying periods of time during 54 months, in contact pathway-inhibited whole blood at 37°C and evaluated in terms of the activation of various substrates. After quenching over time with inhibitors, the soluble phases were analyzed for thrombin-antithrombin III (TAT) complex formation, prothrombin fragments, platelet activation (osteonectin release), factor Va generation, fibrinopeptide (FP) A and FPB release, and factor XIII activation. TAT complex formation, for 35 experiments, showed an initiation phase (up to 4.6 ± 0.6 minutes) in which thrombin was generated at an average rate of 0.93 ± 0.3 nM/min catalyzed by about 1.3 pM prothrombinase yielding approximately 26 nM thrombin. During a subsequent propagation phase, thrombin was generated at a rate of 83.9 ± 3.8 nM/min by about 120 pM prothrombinase, reaching ultimate levels of 851 ± 53 nM. Clot time, determined subjectively, occurred at 4.7 ± 0.2 minutes and correlated with the inception of the propagation phase. The thrombin concentrations associated with the transitions to rapid product formation are 510 ± 180 pM for platelet activation (1.9 ± 0.2 minutes), 840 ± 280 pM for factor XIII activation and factor Va generation (2.2 ± 0.6 minutes), 1.3 ± 0.4 nM for FPA release (2.5 ± 0.7 minutes), 1.7 ± 0.5 nM for FPB release and prethrombin 2 (2.8 ± 0.8 minutes), 7.0 ± 2.2 nM for thrombin B chain (3.6 ± 0.2 minutes), and 26 ± 6.2 nM for the propagation phase of TAT formation (4.6 ± 0.6 minutes). These results illustrate that the initial activation of thrombin substrates occurs during the initiation phase at less than 2 nM thrombin (0.2%). Most thrombin (96%) is formed well after clotting occurs. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Letter in Blood Online: Factor XIII activation by thrombin depends on FXIIIVal34Leu genotype Verena Schroeder, Hans P. Kohler, Kathleen E. Brummel, and Kenneth G. Mann Blood 2003 101: 371. [Full Text] [PDF] This article has been cited by other articles: F. Van de Werf New antithrombotic agents: are they needed and what can they offer to patients with a non-ST-elevation acute coronary syndrome? Eur. Heart J., July 2, 2009; 30(14): 1695 - 1702. [Abstract] [Full Text] [PDF] A. Undas, K. Szuldrzynski, K. E. Brummel-Ziedins, W. Tracz, K. Zmudka, and K. G. Mann Systemic blood coagulation activation in acute coronary syndromes Blood, February 26, 2009; 113(9): 2070 - 2078. [Abstract] [Full Text] [PDF] P. Kamath and S. Krishnaswamy Fate of Membrane-bound Reactants and Products during the Activation of Human Prothrombin by Prothrombinase J. Biol. Chem., October 31, 2008; 283(44): 30164 - 30173. [Abstract] [Full Text] [PDF] T. Orfeo, K. E. Brummel-Ziedins, M. Gissel, S. Butenas, and K. G. Mann The Nature of the Stable Blood Clot Procoagulant Activities J. Biol. Chem., April 11, 2008; 283(15): 9776 - 9786. [Abstract] [Full Text] [PDF] P. A. Gurbel, R. C. Becker, K. G. Mann, S. R. Steinhubl, and A. D. Michelson Platelet Function Monitoring in Patients With Coronary Artery Disease J. Am. Coll. Cardiol., November 6, 2007; 50(19): 1822 - 1834. [Abstract] [Full Text] [PDF] M. Mittermayr, W. Streif, T. Haas, D. Fries, C. Velik-Salchner, A. Klingler, E. Oswald, C. Bach, M. Schnapka-Koepf, and P. Innerhofer Hemostatic Changes After Crystalloid or Colloid Fluid Administration During Major Orthopedic Surgery: The Role of Fibrinogen Administration Anesth. Analg., October 1, 2007; 105(4): 905 - 917. [Abstract] [Full Text] [PDF] K. A. Tanaka, F. Szlam, H. Y. Sun, T. Taketomi, and J. H. Levy Thrombin Generation Assay and Viscoelastic Coagulation Monitors Demonstrate Differences in the Mode of Thrombin Inhibition Between Unfractionated Heparin and Bivalirudin Anesth. Analg., October 1, 2007; 105(4): 933 - 939. [Abstract] [Full Text] [PDF] C.S. Vieira, R.A. Ferriani, A.A. Garcia, M.C. Pintao, G.D. Azevedo, M.K.O. Gomes, and M.F. Silva-de-Sa Use of the etonogestrel-releasing implant is associated with hypoactivation of the coagulation cascade Hum. Reprod., August 1, 2007; 22(8): 2196 - 2201. [Abstract] [Full Text] [PDF] T J Coats, E Brazil, M Heron, and P K MacCallum Impairment of coagulation by commonly used resuscitation fluids in human volunteers. Emerg. Med. J., November 1, 2006; 23(11): 846 - 849. [Abstract] [Full Text] [PDF] T J Coats, E Brazil, and M Heron The effects of commonly used resuscitation fluids on whole blood coagulation. Emerg. Med. J., July 1, 2006; 23(7): 546 - 549. [Abstract] [Full Text] [PDF] N. J. Chao Umbilical Cord Blood: Biology and Transplantation Am. Assoc. Cancer Res. Educ. Book, April 1, 2006; 2006(1): 329 - 333. [Full Text] [PDF] T. Orfeo, S. Butenas, K. E. Brummel-Ziedins, and K. G. Mann The Tissue Factor Requirement in Blood Coagulation J. Biol. Chem., December 30, 2005; 280(52): 42887 - 42896. [Abstract] [Full Text] [PDF] V. B. Louvain-Quintard, E. P. Bianchini, C. Calmel-Tareau, M. Tagzirt, and B. F. Le Bonniec Thrombin-activable Factor X Re-establishes an Intrinsic Amplification in Tenase-deficient Plasmas J. Biol. Chem., December 16, 2005; 280(50): 41352 - 41359. [Abstract] [Full Text] [PDF] D. A. Lane, H. Philippou, and J. A. Huntington Directing thrombin Blood, October 15, 2005; 106(8): 2605 - 2612. [Abstract] [Full Text] [PDF] S. Butenas, B. A. Bouchard, K. E. Brummel-Ziedins, B. Parhami-Seren, and K. G. Mann Tissue factor activity in whole blood Blood, April 1, 2005; 105(7): 2764 - 2770. [Abstract] [Full Text] [PDF] J. Kawasaki, N. Katori, M. Kodaka, H. Miyao, and K. A. Tanaka Electron Microscopic Evaluations of Clot Morphology During Thrombelastography(R) Anesth. Analg., November 1, 2004; 99(5): 1440 - 1444. [Abstract] [Full Text] [PDF] K. A. Tanaka, N. Katori, F. Szlam, N. Sato, A. B. Kelly, and J. H. Levy Effects of tirofiban on haemostatic activation in vitro Br. J. Anaesth., August 1, 2004; 93(2): 263 - 269. [Abstract] [Full Text] [PDF] S. Butenas, T. Orfeo, M. T. Gissel, K. E. Brummel, and K. G. Mann The Significance of Circulating Factor IXa in Blood J. Biol. Chem., May 28, 2004; 279(22): 22875 - 22882. [Abstract] [Full Text] [PDF] T. Orfeo, N. Brufatto, M. E. Nesheim, H. Xu, S. Butenas, and K. G. Mann The Factor V Activation Paradox J. Biol. Chem., May 7, 2004; 279(19): 19580 - 19591. [Abstract] [Full Text] [PDF] N. J. Chao, S. G. Emerson, and K. I. Weinberg Stem Cell Transplantation (Cord Blood Transplants) Hematology, January 1, 2004; 2004(1): 354 - 371. [Abstract] [Full Text] [PDF] K. G. Mann Thrombin Formation Chest, September 1, 2003; 124 (2009): 4S - 10S. [Abstract] [Full Text] [PDF] M. Nesheim Thrombin and Fibrinolysis Chest, September 1, 2003; 124 (2009): 33S - 39S. [Abstract] [Full Text] [PDF] M. Kalafatis, D. O. Beck, and K. G. Mann Structural Requirements for Expression of Factor Va Activity J. Biol. Chem., August 29, 2003; 278(35): 33550 - 33561. [Abstract] [Full Text] [PDF] W. L. Chandler and T. Velan Estimating the rate of thrombin and fibrin generation in vivo during cardiopulmonary bypass Blood, June 1, 2003; 101(11): 4355 - 4362. [Abstract] [Full Text] [PDF] K. G. Mann, S. Butenas, and K. Brummel The Dynamics of Thrombin Formation Arterioscler Thromb Vasc Biol, January 1, 2003; 23(1): 17 - 25. [Abstract] [Full Text] [PDF] S. Butenas, K. E. Brummel, S. G. Paradis, and K. G. Mann Influence of Factor VIIa and Phospholipids on Coagulation in "Acquired" Hemophilia Arterioscler Thromb Vasc Biol, January 1, 2003; 23(1): 123 - 129. [Abstract] [Full Text] [PDF] V. Schroeder, H. P. Kohler, K. E. Brummel, and K. G. Mann Factor XIII activation by thrombin depends on FXIIIVal34Leu genotype Blood, January 1, 2003; 101(1): 371 - 371. [Full Text] [PDF] K. G. Mann and M. Kalafatis Factor V: a combination of Dr Jekyll and Mr Hyde Blood, January 1, 2003; 101(1): 20 - 30. [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020