SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Canonne-Hergaux, F. Articles by Gros, P. Search for Related Content PubMed PubMed Citation Articles by Canonne-Hergaux, F. Articles by Gros, P. Related Collections Phagocytes Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 July 2002, Vol. 100, No. 1, pp. 268-275 PHAGOCYTES Expression and subcellular localization of NRAMP1 in human neutrophil granules François Canonne-Hergaux, Jero Calafat, Etienne Richer, Mathieu Cellier, Sergio Grinstein, Neils Borregaard, and Philippe Gros From the Department of Biochemistry, Center for the Study of Host Resistance, McGill Cancer Center, McGill University, Montreal, QC, Canada; the Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, Netherlands; INRS-Institut Armand-Frappier, Laval, QC, Canada; the Division of Cell Biology, The Hospital for Sick Children, Toronto, Canada; and the Department of Hematology, University of Copenhagen, Copenhagen, Denmark. Mutations at the Nramp1 gene cause susceptibility to infections with intracellular pathogens. In human blood, polymorphonuclear (PMN) leukocytes are the most abundant site of NRAMP1 messenger RNA (mRNA) expression, suggesting that NRAMP1 plays an important role in the activity of these cells. By Northern blot analysis, NRAMP1 mRNA was only detected in most mature neutrophils from bone marrow (band and segmented cells). A high-affinity polyclonal rabbit antihuman NRAMP1 antibody directed against the amino terminus of the protein was produced and used to study cellular and subcellular localization of the protein in primary human neutrophils. Subcellular fractionation of granule populations together with immunoblotting studies with granule-specific markers indicate that NRAMP1 expression is primarily in tertiary granules. These granules are positive for the matrix enzyme gelatinase and the membrane subunit of the vacuolar H+/ATPase and can be recruited for exocytosis by treatment of neutrophils with phorbol myristate acetate. Immunogold studies by cryoelectron microscopy with primary neutrophils confirm that a majority (75%) of NRAMP1-positive granules are also positive for gelatinase, but they also suggest further heterogeneity in this granule population. Presence of NRAMP1 in tertiary granules is in agreement with the late-stage appearance of NRAMP1 mRNA during neutrophil maturation in bone marrow. Finally, immunofluorescence studies of Candida albicans-containing phagosomes formed in neutrophils indicate that NRAMP1 is recruited from tertiary granules to the phagosomal membrane on phagocytosis, supporting a role for NRAMP1 in the antimicrobial defenses of human neutrophils. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H.-R. Jiang, D. S. Gilchrist, J.-F. Popoff, S. E. Jamieson, M. Truscott, J. K. White, and J. M. Blackwell Influence of Slc11a1 (formerly Nramp1) on DSS-induced colitis in mice J. Leukoc. Biol., April 1, 2009; 85(4): 703 - 710. [Abstract] [Full Text] [PDF] M. A. Gomez, S. Li, M. L. Tremblay, and M. Olivier NRAMP-1 Expression Modulates Protein-tyrosine Phosphatase Activity in Macrophages: IMPACT ON HOST CELL SIGNALING AND FUNCTIONS J. Biol. Chem., December 14, 2007; 282(50): 36190 - 36198. [Abstract] [Full Text] [PDF] M. E. Techau, J. Valdez-Taubas, J.-F. Popoff, R. Francis, M. Seaman, and J. M. Blackwell Evolution of Differences in Transport Function in Slc11a Family Members J. Biol. Chem., December 7, 2007; 282(49): 35646 - 35656. [Abstract] [Full Text] [PDF] H. K. Bayele, C. Peyssonnaux, A. Giatromanolaki, W. W. Arrais-Silva, H. S. Mohamed, H. Collins, S. Giorgio, M. Koukourakis, R. S. Johnson, J. M. Blackwell, et al. HIF-1 regulates heritable variation and allele expression phenotypes of the macrophage immune response gene SLC11A1 from a Z-DNA forming microsatellite Blood, October 15, 2007; 110(8): 3039 - 3048. [Abstract] [Full Text] [PDF] C. B. Stober, S. Brode, J. K. White, J.-F. Popoff, and J. M. Blackwell Slc11a1, Formerly Nramp1, Is Expressed in Dendritic Cells and Influences Major Histocompatibility Complex Class II Expression and Antigen-Presenting Cell Function Infect. Immun., October 1, 2007; 75(10): 5059 - 5067. [Abstract] [Full Text] [PDF] K. Turcotte, S. Gauthier, D. Malo, M. Tam, M. M. Stevenson, and P. Gros Icsbp1/IRF-8 Is Required for Innate and Adaptive Immune Responses against Intracellular Pathogens J. Immunol., August 15, 2007; 179(4): 2467 - 2476. [Abstract] [Full Text] [PDF] H. J. Knowles, D. R. Mole, P. J. Ratcliffe, and A. L. Harris Normoxic Stabilization of Hypoxia-Inducible Factor-1{alpha} by Modulation of the Labile Iron Pool in Differentiating U937 Macrophages: Effect of Natural Resistance-Associated Macrophage Protein 1. Cancer Res., March 1, 2006; 66(5): 2600 - 2607. [Abstract] [Full Text] [PDF] A. Fortier, G. Min-Oo, J. Forbes, S. Lam-Yuk-Tseung, and P. Gros Single gene effects in mouse models of host: pathogen interactions J. Leukoc. Biol., June 1, 2005; 77(6): 868 - 877. [Abstract] [Full Text] [PDF] P. Massullo, L. J. Druhan, B. A. Bunnell, M. G. Hunter, J. M. Robinson, C. B. Marsh, and B. R. Avalos Aberrant subcellular targeting of the G185R neutrophil elastase mutant associated with severe congenital neutropenia induces premature apoptosis of differentiating promyelocytes Blood, May 1, 2005; 105(9): 3397 - 3404. [Abstract] [Full Text] [PDF] N. Borregaard, K. Theilgaard-Monch, J. B. Cowland, M. Stahle, and O. E. Sorensen Neutrophils and keratinocytes in innate immunity--cooperative actions to provide antimicrobial defense at the right time and place J. Leukoc. Biol., April 1, 2005; 77(4): 439 - 443. [Abstract] [Full Text] [PDF] K. Turcotte, S. Gauthier, L.-M. Mitsos, C. Shustik, N. G. Copeland, N. A. Jenkins, J.-C. Fournet, P. Jolicoeur, and P. Gros Genetic control of myeloproliferation in BXH-2 mice Blood, March 15, 2004; 103(6): 2343 - 2350. [Abstract] [Full Text] [PDF] J. R. Forbes and P. Gros Iron, manganese, and cobalt transport by Nramp1 (Slc11a1) and Nramp2 (Slc11a2) expressed at the plasma membrane Blood, September 1, 2003; 102(5): 1884 - 1892. [Abstract] [Full Text] [PDF] N. Jabado, P. Cuellar-Mata, S. Grinstein, and P. Gros Iron chelators modulate the fusogenic properties of Salmonella-containing phagosomes PNAS, May 13, 2003; 100(10): 6127 - 6132. [Abstract] [Full Text] [PDF] L. A. Guilloteau, J. Dornand, A. Gross, M. Olivier, F. Cortade, Y. L. Vern, and D. Kerboeuf Nramp1 Is Not a Major Determinant in the Control of Brucella melitensis Infection in Mice Infect. Immun., February 1, 2003; 71(2): 621 - 628. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020