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Prepublished online as a Blood First Edition Paper on July 25, 2002; DOI 10.1182/blood-2002-01-0303.
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Blood, 15 November 2002, Vol. 100, No. 10, pp. 3597-3603
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Differential movements of VE-cadherin and PECAM-1 during
transmigration of polymorphonuclear leukocytes through human
umbilical vein endothelium
Wen-Hong Su,
Hsiun-ing Chen, and
Chauying J. Jen
From the Department of Physiology and Institute of
Basic Medical Sciences, College of Medicine, National Cheng-Kung
University, Tainan, Taiwan, Republic of China.
Most existing evidence regarding junction protein movements during
transendothelial migration of leukocytes comes from taking postfixation
snap shots of the transendothelial migration process that happens on a
cultured endothelial monolayer. In this study, we used junction
protein-specific antibodies that did not interfere with the
transendothelial migration to examine the real-time movements of
vascular endothelial-cadherin (VE-cadherin) and platelet/endothelial cell adhesion molecule-1 (PECAM-1) during transmigration of
polymorphonuclear leukocytes (PMNs) either through a cultured
endothelial monolayer or through the endothelium of dissected human
umbilical vein tissue. In either experimental model system, both
junction proteins showed relative movements, not transient
disappearance, at the PMN transmigration sites. VE-cadherin moved away
to different ends of the transmigration site, whereas PECAM-1 opened to
surround the periphery of a transmigrating PMN. Junction proteins
usually moved back to their original positions when the PMN
transmigration process was completed in less than 2 minutes. The
relative positions of some junction proteins might rearrange to form a
new interendothelial contour after PMNs had transmigrated through
multicellular corners. Although transmigrated PMNs maintained good
mobility, they only moved laterally underneath the vascular endothelium
instead of deeply into the vascular tissue. In conclusion, our results
obtained from using either cultured cells or vascular tissues showed
that VE-cadherin-containing adherent junctions were relocated aside,
not opened or disrupted, whereas PECAM-1-containing junctions were
opened during PMN transendothelial migration.
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