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Prepublished online as a Blood First Edition Paper on July 5, 2002; DOI 10.1182/blood-2002-03-0937.
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Blood, 15 November 2002, Vol. 100, No. 10, pp. 3828-3831
BRIEF REPORT
Immortalization of yolk sac-derived precursor
cells
Wen-Mei Yu,
Teresa S. Hawley,
Robert G. Hawley, and
Cheng-Kui Qu
From the Department of Hematopoiesis and the Flow
Cytometry Facility, Jerome H. Holland Laboratory for the Biomedical
Sciences, American Red Cross, Rockville, MD, and Department of Anatomy
and Cell Biology, The George Washington University Medical Center,
Washington, DC.
Hematopoiesis initiates in the extraembryonic yolk sac. To
isolate various types of precursor cells from this blood cell-forming tissue, yolk sac cells were immortalized by retroviral-mediated expression of the HOX11 homeobox-containing gene. Among the
cell lines derived, some were able to spontaneously generate adherent stromal-like cells capable of taking up acetylated low-density lipoprotein, and they could be induced to form tubelike structures when
cultured on Matrigel. Although these cell lines were negative for
hematopoietic cell surface markers, they gave rise to hematopoietic colonies containing cells belonging to the monocytic,
megakaryocytic, and definitive erythroid lineageswhen plated in
methylcellulose medium supplemented with hematopoietic growth factors.
Low amounts of Flk-1 mRNA could be detected in these cells, and they
showed significant responsiveness to vascular endothelial growth
factor, stem cell factor, basic fibroblast growth factor, and
interleukin 6. They also expressed the transcription factors SCL,
GATA2, GATA1, PU.1, and c-myb. These yolk
sac-derived cell lines may represent a transitional stage of early
hematopoietic development.
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