SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Botella, L. M. Articles by Bernabéu, C. Search for Related Content PubMed PubMed Citation Articles by Botella, L. M. Articles by Bernabéu, C. Related Collections Hemostasis, Thrombosis, and Vascular Biology Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 December 2002, Vol. 100, No. 12, pp. 4001-4010 HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Transcriptional activation of endoglin and transforming growth factor- signaling components by cooperative interaction between Sp1 and KLF6: their potential role in the response to vascular injury Luisa M. Botella, Tilman Sánchez-Elsner, Francisco Sanz-Rodriguez, Soichi Kojima, Jun Shimada, Mercedes Guerrero-Esteo, Michael P. Cooreman, Vlad Ratziu, Carmen Langa, Calvin P. H. Vary, Jose R. Ramírez, Scott Friedman, and Carmelo Bernabéu From the Centro de Investigaciones Biológicas, Consejo Superior Investigaciones Cientificas (CSIC), Madrid, Spain; Laboratory of Molecular Cell Sciences, Riken, Wako, Saitama, Japan; Servicio Anatomía Patológica, Hospital Gómez Ulla, Madrid, Spain; Division of Liver Diseases, Department of Medicine, Mount Sinai School of Medicine, New York, NY; and Maine Medical Center Research Institute, Scarborough, ME. Endoglin is an endothelial membrane glycoprotein involved in cardiovascular morphogenesis and vascular remodeling. It associates with transforming growth factor- (TGF-) signaling receptors to bind TGF- family members, forming a functional receptor complex. Arterial injury leads to up-regulation of endoglin, but the underlying regulatory events are unknown. The transcription factor KLF6, an immediate-early response gene induced in endothelial cells during vascular injury, transactivates TGF-, TGF- signaling receptors, and TGF--stimulated genes. KLF6 and, subsequently, endoglin were colocalized to vascular endothelium (ie, expressed in the same cell type) following carotid balloon injury in rats. After endothelial denudation, KLF6 was induced and translocated to the nucleus; this was followed 6 hours later by increased endoglin expression. Transient overexpression of KLF6, but not Egr-1, stimulated endogenous endoglin mRNA and transactivated the endoglin promoter. This transactivation was dependent on a GC-rich tract required for basal activity of the endoglin promoter driven by the related GC box binding protein, Sp1. In cells lacking Sp1 and KLF6, transfected KLF6 and Sp1 cooperatively transactivated the endoglin promoter and those of collagen 1(I), urokinase-type plasminogen activator, TGF-1, and TGF- receptor type 1. Direct physical interaction between Sp1 and KLF6 was documented by coimmunoprecipitation, pull-down experiments, and the GAL4 one-hybrid system, mapping the KLF6 interaction to the C-terminal domain of Sp1. These data provide evidence that injury-induced KLF6 and preexisting Sp1 may cooperate in regulating the expression of endoglin and related members of the TGF- signaling complex in vascular repair. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: W. Qi, X. Chen, J. Holian, C. Y.R. Tan, D. J. Kelly, and C. A. Pollock Transcription Factors Kruppel-Like Factor 6 and Peroxisome Proliferator-Activated Receptor-{gamma} Mediate High Glucose-Induced Thioredoxin-Interacting Protein Am. J. Pathol., November 1, 2009; 175(5): 1858 - 1867. [Abstract] [Full Text] [PDF] B. Rockx, T. Baas, G. A. Zornetzer, B. Haagmans, T. Sheahan, M. Frieman, M. D. Dyer, T. H. Teal, S. Proll, J. van den Brand, et al. Early Upregulation of Acute Respiratory Distress Syndrome-Associated Cytokines Promotes Lethal Disease in an Aged-Mouse Model of Severe Acute Respiratory Syndrome Coronavirus Infection J. Virol., July 15, 2009; 83(14): 7062 - 7074. [Abstract] [Full Text] [PDF] S. M. Meadows, M. C. Salanga, and P. A. Krieg Kruppel-like factor 2 cooperates with the ETS family protein ERG to activate Flk1 expression during vascular development Development, April 1, 2009; 136(7): 1115 - 1125. [Abstract] [Full Text] [PDF] Y. N. Ohnishi, K. Sakumi, K. Yamazaki, Y. H. Ohnishi, T. Miura, Y. Tominaga, and Y. Nakabeppu Antagonistic Regulation of Cell-Matrix Adhesion by FosB and {Delta}FosB/{Delta}2{Delta}FosB Encoded by Alternatively Spliced Forms of fosB Transcripts Mol. Biol. Cell, November 1, 2008; 19(11): 4717 - 4729. [Abstract] [Full Text] [PDF] I. Bentov, G. Narla, H. Schayek, K. Akita, S. R. Plymate, D. LeRoith, S. L. Friedman, and H. Werner Insulin-Like Growth Factor-I Regulates Kruppel-Like Factor-6 Gene Expression in a p53-Dependent Manner Endocrinology, April 1, 2008; 149(4): 1890 - 1897. [Abstract] [Full Text] [PDF] S. L. Friedman Hepatic Stellate Cells: Protean, Multifunctional, and Enigmatic Cells of the Liver Physiol Rev, January 1, 2008; 88(1): 125 - 172. [Abstract] [Full Text] [PDF] K. Enya, H. Hayashi, T. Takii, N. Ohoka, S. Kanata, T. Okamoto, and K. Onozaki The interaction with Sp1 and reduction in the activity of histone deacetylase 1 are critical for the constitutive gene expression of IL-1{alpha} in human melanoma cells J. Leukoc. Biol., January 1, 2008; 83(1): 190 - 199. [Abstract] [Full Text] [PDF] G. B. Atkins and M. K. Jain Role of Kruppel-Like Transcription Factors in Endothelial Biology Circ. Res., June 22, 2007; 100(12): 1686 - 1695. [Abstract] [Full Text] [PDF] J. E. Pimanda, W.Y. I. Chan, I. J. Donaldson, M. Bowen, A. R. Green, and B. Gottgens Endoglin expression in the endothelium is regulated by Fli-1, Erg, and Elf-1 acting on the promoter and a -8-kb enhancer Blood, June 15, 2006; 107(12): 4737 - 4745. [Abstract] [Full Text] [PDF] F. Chiambaretta, H. Nakamura, F. De Graeve, H. Sakai, G. Marceau, Y. Maruyama, D. Rigal, B. Dastugue, J. Sugar, B. Y. J. T. Yue, et al. Kruppel-like Factor 6 (KLF6) Affects the Promoter Activity of the {alpha}1-Proteinase Inhibitor Gene Invest. Ophthalmol. Vis. Sci., February 1, 2006; 47(2): 582 - 590. [Abstract] [Full Text] [PDF] D. Li, S. Yea, G. Dolios, J. A. Martignetti, G. Narla, R. Wang, M. J. Walsh, and S. L. Friedman Regulation of Kruppel-like Factor 6 Tumor Suppressor Activity by Acetylation Cancer Res., October 15, 2005; 65(20): 9216 - 9225. [Abstract] [Full Text] [PDF] S. Sen-Banerjee, S. Mir, Z. Lin, A. Hamik, G. B. Atkins, H. Das, P. Banerjee, A. Kumar, and M. K. Jain Kruppel-Like Factor 2 as a Novel Mediator of Statin Effects in Endothelial Cells Circulation, August 2, 2005; 112(5): 720 - 726. [Abstract] [Full Text] [PDF] D. Li, S. Yea, S. Li, Z. Chen, G. Narla, M. Banck, J. Laborda, S. Tan, J. M. Friedman, S. L. Friedman, et al. Kruppel-like Factor-6 Promotes Preadipocyte Differentiation through Histone Deacetylase 3-dependent Repression of DLK1 J. Biol. Chem., July 22, 2005; 280(29): 26941 - 26952. [Abstract] [Full Text] [PDF] T. Onoe, H. Ohdan, D. Tokita, M. Shishida, Y. Tanaka, H. Hara, W. Zhou, K. Ishiyama, H. Mitsuta, K. Ide, et al. Liver Sinusoidal Endothelial Cells Tolerize T Cells across MHC Barriers in Mice J. Immunol., July 1, 2005; 175(1): 139 - 146. [Abstract] [Full Text] [PDF] T. Suzuki, K. Aizawa, T. Matsumura, and R. Nagai Vascular Implications of the Kruppel-Like Family of Transcription Factors Arterioscler Thromb Vasc Biol, June 1, 2005; 25(6): 1135 - 1141. [Abstract] [Full Text] [PDF] M. van de Bovenkamp, G. M. M. Groothuis, A. L. Draaisma, M. T. Merema, J. I. Bezuijen, M. J. van Gils, D. K. F. Meijer, S. L. Friedman, and P. Olinga Precision-Cut Liver Slices as a New Model to Study Toxicity-Induced Hepatic Stellate Cell Activation in a Physiologic Milieu Toxicol. Sci., May 1, 2005; 85(1): 632 - 638. [Abstract] [Full Text] [PDF] H. Nakamura, F. Chiambaretta, J. Sugar, V. Sapin, and B. Y. J. T. Yue Developmentally Regulated Expression of KLF6 in the Mouse Cornea and Lens Invest. Ophthalmol. Vis. Sci., December 1, 2004; 45(12): 4327 - 4332. [Abstract] [Full Text] [PDF] F. Sanz-Rodriguez, A. Fernandez-L., R. Zarrabeitia, A. Perez-Molino, J. R. Ramirez, E. Coto, C. Bernabeu, and L. M. Botella Mutation Analysis in Spanish Patients with Hereditary Hemorrhagic Telangiectasia: Deficient Endoglin Up-regulation in Activated Monocytes Clin. Chem., November 1, 2004; 50(11): 2003 - 2011. [Abstract] [Full Text] [PDF] M. Rubinstein, G. Idelman, S. R. Plymate, G. Narla, S. L. Friedman, and H. Werner Transcriptional Activation of the Insulin-Like Growth Factor I Receptor Gene by the Kruppel-Like Factor 6 (KLF6) Tumor Suppressor Protein: Potential Interactions between KLF6 and p53 Endocrinology, August 1, 2004; 145(8): 3769 - 3777. [Abstract] [Full Text] [PDF] J. A. Potian, H. Aviv, N. M. Ponzio, J. S. Harrison, and P. Rameshwar Veto-Like Activity of Mesenchymal Stem Cells: Functional Discrimination Between Cellular Responses to Alloantigens and Recall Antigens J. Immunol., October 1, 2003; 171(7): 3426 - 3434. [Abstract] [Full Text] [PDF] G. Narla, S. L. Friedman, and J. A. Martignetti Kruppel Cripples Prostate Cancer: KLF6 Progress and Prospects Am. J. Pathol., April 1, 2003; 162(4): 1047 - 1052. [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020