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Prepublished online as a Blood First Edition Paper on August 15, 2002; DOI 10.1182/blood-2001-11-0097.
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Blood, 15 December 2002, Vol. 100, No. 13, pp. 4512-4520
IMMUNOBIOLOGY
Characterization of human blood dendritic cell subsets
Kelli P. A. MacDonald,
David J. Munster,
Georgina J. Clark,
Andrzej Dzionek,
Juergen Schmitz, and
Derek N. J. Hart
From the Dendritic Cell Laboratory, Mater Medical
Research Institute, Mater Misericordiae Hospitals, South Brisbane,
Australia; Miltenyi Biotec GmbH, Bergisch Gladbach,
Germany.
Dendritic cells (DCs) are key antigen-presenting cells for
stimulating immune responses and they are now being investigated in
clinical settings. Although defined as lineage-negative
(Lin ) HLA-DR+ cells, significant
heterogeneity in these preparations is apparent, particularly in regard
to the inclusion or exclusion of CD14+, CD16+,
and CD2+ cells. This study used flow cytometry and a panel
of monoclonal antibodies (mAbs), including reagents from the 7th
Leukocyte Differentiation Antigen Workshop, to define the cellular
composition of 2 standardized peripheral blood mononuclear cell
(PBMCs)-derived Lin HLA-DR+
preparations. Lin cells were prepared from PBMCs by
depletion with CD3, CD14, CD19, CD11b, and either CD16 or CD56 mAbs.
Analysis of the CD16-replete preparations divided the
Lin HLA-DR+ population into 5 nonoverlapping
subsets (mean ± 1 SD): CD123 (mean = 18.3% ± 9.7%), CD1b/c
(18.6% ± 7.6%), CD16 (49.6% ± 8.5%), BDCA-3 (2.7% ±
1.4%), and CD34 (5.0% ± 2.4%). The 5 subsets had distinct
phenotypes when compared with each other, monocytes, and
monocyte-derived DCs (MoDCs). The CD85 family, C-type lectins, costimulatory molecules, and differentiation/activation molecules were
also expressed differentially on the 5 Lin
HLA-DR+ subsets, monocytes, and MoDCs. The poor viability
of CD123+ DCs in vitro was confirmed, but the
CD16+ CD11c+ DC subset also survived poorly.
Finally, the individual subsets used as stimulators in allogeneic mixed
leukocyte reactions were ranked by their allostimulatory capacity as
CD1b/c > CD16 > BDCA-3 > CD123 > CD34. These data provide
an opportunity to standardize the DC populations used for future
molecular, functional and possibly even therapeutic studies.
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|
|
|
|
|
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|
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|
|
|
|
|
|
|
S. Yamagami, S. Yokoo, T. Usui, H. Yamagami, S. Amano, and N. Ebihara
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|
|
|
|
|
|
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M. Lindstedt, K. Lundberg, and C. A. K. Borrebaeck
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175(8):
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|
|
|
|
|
|
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B. Morandi, R. Costa, M. Falco, S. Parolini, A. De Maria, G. Ratto, M. C. Mingari, G. Melioli, A. Moretta, and G. Ferlazzo
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175(6):
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[Full Text]
[PDF]
|
|
|
|
|
|
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D. McIlroy, S. Tanguy-Royer, N. Le Meur, I. Guisle, P.-J. Royer, J. Leger, K. Meflah, and M. Gregoire
Profiling dendritic cell maturation with dedicated microarrays
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78(3):
794 - 803.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Dalgaard, K. J. Beckstrom, F. L. Jahnsen, and J. E. Brinchmann
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77(5):
689 - 698.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Schmitz, S. Zhao, Y. Deuse, K. Schakel, R. Wehner, H. Wohner, K. Holig, F. Wienforth, A. Kiessling, M. Bornhauser, et al.
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174(7):
4127 - 4134.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Vuckovic, D. Khalil, N. Angel, F. Jahnsen, I. Hamilton, A. Boyce, B. Hock, and D. N. J. Hart
The CMRF58 antibody recognizes a subset of CD123hi dendritic cells in allergen-challenged mucosa
J. Leukoc. Biol.,
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77(3):
344 - 351.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Hashizume, T. Horibe, H. Yagi, N. Seo, and M. Takigawa
Compartmental Imbalance and Aberrant Immune Function of Blood CD123+ (Plasmacytoid) and CD11c+ (Myeloid) Dendritic Cells in Atopic Dermatitis
J. Immunol.,
February 15, 2005;
174(4):
2396 - 2403.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. Smyth, G. Logan, R. Boadle, P. B. Rowe, J. A. Smythe, and I. E. Alexander
Differential subcellular localization of CD86 in human PBMC-derived macrophages and DCs, and ultrastructural characterization by immuno-electron microscopy
Int. Immunol.,
February 1, 2005;
17(2):
123 - 132.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Zabel, A. M. Silverio, and E. C. Butcher
Chemokine-Like Receptor 1 Expression and Chemerin-Directed Chemotaxis Distinguish Plasmacytoid from Myeloid Dendritic Cells in Human Blood
J. Immunol.,
January 1, 2005;
174(1):
244 - 251.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. F. Fagnoni, B. Oliviero, G. Giorgiani, P. De Stefano, A. Deho, C. Zibera, N. Gibelli, R. Maccario, G. Da Prada, M. Zecca, et al.
Reconstitution dynamics of plasmacytoid and myeloid dendritic cell precursors after allogeneic myeloablative hematopoietic stem cell transplantation
Blood,
July 1, 2004;
104(1):
281 - 289.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Reddy, J. A. Iturraspe, A. C. Tzolas, H.-U. Meier-Kriesche, J. Schold, and J. R. Wingard
Low dendritic cell count after allogeneic hematopoietic stem cell transplantation predicts relapse, death, and acute graft-versus-host disease
Blood,
June 1, 2004;
103(11):
4330 - 4335.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Fujiwara, F. El Ouriaghli, M. Grube, D. A. Price, K. Rezvani, E. Gostick, G. Sconocchia, J. Melenhorst, N. Hensel, D. C. Douek, et al.
Identification and in vitro expansion of CD4+ and CD8+ T cells specific for human neutrophil elastase
Blood,
April 15, 2004;
103(8):
3076 - 3083.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Munster, K. P. A. MacDonald, M. Kato, and D. J. N. Hart
Human T lymphoblasts and activated dendritic cells in the allogeneic mixed leukocyte reaction are susceptible to NK cell-mediated anti-CD83-dependent cytotoxicity
Int. Immunol.,
January 1, 2004;
16(1):
33 - 42.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Turville, J. Wilkinson, P. Cameron, J. Dable, and A. L. Cunningham
The role of dendritic cell C-type lectin receptors in HIV pathogenesis
J. Leukoc. Biol.,
November 1, 2003;
74(5):
710 - 718.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Teleshova, I. Frank, and M. Pope
Immunodeficiency virus exploitation of dendritic cells in the early steps of infection
J. Leukoc. Biol.,
November 1, 2003;
74(5):
683 - 690.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Orsini, A. Guarini, S. Chiaretti, F. R. Mauro, and R. Foa
The Circulating Dendritic Cell Compartment in Patients with Chronic Lymphocytic Leukemia Is Severely Defective and Unable to Stimulate an Effective T-Cell Response
Cancer Res.,
August 1, 2003;
63(15):
4497 - 4506.
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