SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on August 15, 2002; DOI 10.1182/blood-2001-11-0097. This Article Full Text Full Text (PDF) All Versions of this Article: 2001-11-0097v1 100/13/4512    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by MacDonald, K. P. A. Articles by Hart, D. N. J. Search for Related Content PubMed PubMed Citation Articles by MacDonald, K. P. A. Articles by Hart, D. N. J. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 December 2002, Vol. 100, No. 13, pp. 4512-4520 IMMUNOBIOLOGY Characterization of human blood dendritic cell subsets Kelli P. A. MacDonald, David J. Munster, Georgina J. Clark, Andrzej Dzionek, Juergen Schmitz, and Derek N. J. Hart From the Dendritic Cell Laboratory, Mater Medical Research Institute, Mater Misericordiae Hospitals, South Brisbane, Australia; Miltenyi Biotec GmbH, Bergisch Gladbach, Germany. Dendritic cells (DCs) are key antigen-presenting cells for stimulating immune responses and they are now being investigated in clinical settings. Although defined as lineage-negative (Lin) HLA-DR+ cells, significant heterogeneity in these preparations is apparent, particularly in regard to the inclusion or exclusion of CD14+, CD16+, and CD2+ cells. This study used flow cytometry and a panel of monoclonal antibodies (mAbs), including reagents from the 7th Leukocyte Differentiation Antigen Workshop, to define the cellular composition of 2 standardized peripheral blood mononuclear cell (PBMCs)-derived Lin HLA-DR+ preparations. Lin cells were prepared from PBMCs by depletion with CD3, CD14, CD19, CD11b, and either CD16 or CD56 mAbs. Analysis of the CD16-replete preparations divided the Lin HLA-DR+ population into 5 nonoverlapping subsets (mean ± 1 SD): CD123 (mean = 18.3% ± 9.7%), CD1b/c (18.6% ± 7.6%), CD16 (49.6% ± 8.5%), BDCA-3 (2.7% ± 1.4%), and CD34 (5.0% ± 2.4%). The 5 subsets had distinct phenotypes when compared with each other, monocytes, and monocyte-derived DCs (MoDCs). The CD85 family, C-type lectins, costimulatory molecules, and differentiation/activation molecules were also expressed differentially on the 5 Lin HLA-DR+ subsets, monocytes, and MoDCs. The poor viability of CD123+ DCs in vitro was confirmed, but the CD16+ CD11c+ DC subset also survived poorly. Finally, the individual subsets used as stimulators in allogeneic mixed leukocyte reactions were ranked by their allostimulatory capacity as CD1b/c > CD16 > BDCA-3 > CD123 > CD34. These data provide an opportunity to standardize the DC populations used for future molecular, functional and possibly even therapeutic studies. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J A Mengshol, L Golden-Mason, N Castelblanco, K A Im, S M Dillon, C C Wilson, H R Rosen, and for the Virahep-C Study Group Impaired plasmacytoid dendritic cell maturation and differential chemotaxis in chronic hepatitis C virus: associations with antiviral treatment outcomes Gut, July 1, 2009; 58(7): 964 - 973. [Abstract] [Full Text] [PDF] J.-O Jin, H.-Y. Park, Q. Xu, J.-I. Park, T. Zvyagintseva, V. A. Stonik, and J.-Y. Kwak Ligand of scavenger receptor class A indirectly induces maturation of human blood dendritic cells via production of tumor necrosis factor-{alpha} Blood, June 4, 2009; 113(23): 5839 - 5847. [Abstract] [Full Text] [PDF] J. Wilson, H. Cullup, R. Lourie, Y. Sheng, A. Palkova, K. J. Radford, A. M. Dickinson, A. M. Rice, D. N.J. Hart, and D. J. Munster Antibody to the dendritic cell surface activation antigen CD83 prevents acute graft-versus-host disease J. Exp. Med., February 16, 2009; 206(2): 387 - 398. [Abstract] [Full Text] [PDF] Z. M. Bamboat, J. A. Stableford, G. Plitas, B. M. Burt, H. M. Nguyen, A. P. Welles, M. Gonen, J. W. Young, and R. P. DeMatteo Human Liver Dendritic Cells Promote T Cell Hyporesponsiveness J. Immunol., February 15, 2009; 182(4): 1901 - 1911. [Abstract] [Full Text] [PDF] C. E. Angel, C.-J. J. Chen, O. C. Horlacher, S. Winkler, T. John, J. Browning, D. MacGregor, J. Cebon, and P. R. Dunbar Distinctive localization of antigen-presenting cells in human lymph nodes Blood, February 5, 2009; 113(6): 1257 - 1267. [Abstract] [Full Text] [PDF] C. Gurer, T. Strowig, F. Brilot, M. Pack, C. Trumpfheller, F. Arrey, C. G. Park, R. M. Steinman, and C. Munz Targeting the nuclear antigen 1 of Epstein-Barr virus to the human endocytic receptor DEC-205 stimulates protective T-cell responses Blood, August 15, 2008; 112(4): 1231 - 1239. [Abstract] [Full Text] [PDF] X. Ju, M. Zenke, D. N. J. Hart, and G. J. Clark CD300a/c regulate type I interferon and TNF-{alpha} secretion by human plasmacytoid dendritic cells stimulated with TLR7 and TLR9 ligands Blood, August 15, 2008; 112(4): 1184 - 1194. [Abstract] [Full Text] [PDF] C. Huysamen, J. A. Willment, K. M. Dennehy, and G. D. Brown CLEC9A Is a Novel Activation C-type Lectin-like Receptor Expressed on BDCA3+ Dendritic Cells and a Subset of Monocytes J. Biol. Chem., June 13, 2008; 283(24): 16693 - 16701. [Abstract] [Full Text] [PDF] E Smolewska, J Stanczyk, H Brozik, M Biernacka-Zielinska, B Cebula, T Robak, and P Smolewski Distribution and clinical significance of blood dendritic cells in children with juvenile idiopathic arthritis Ann Rheum Dis, June 1, 2008; 67(6): 762 - 768. [Abstract] [Full Text] [PDF] G. de la Rosa, D. Yang, P. Tewary, A. Varadhachary, and J. J. Oppenheim Lactoferrin Acts as an Alarmin to Promote the Recruitment and Activation of APCs and Antigen-Specific Immune Responses J. Immunol., May 15, 2008; 180(10): 6868 - 6876. [Abstract] [Full Text] [PDF] M. C. Lebre, S. L. Jongbloed, S. W. Tas, T. J.M. Smeets, I. B. McInnes, and P. P. Tak Rheumatoid Arthritis Synovium Contains Two Subsets of CD83-DC-LAMP- Dendritic Cells with Distinct Cytokine Profiles Am. J. Pathol., April 1, 2008; 172(4): 940 - 950. [Abstract] [Full Text] [PDF] M. I. Verstege, F. J.W. ten Kate, S. M. Reinartz, C. M. van Drunen, F. J.M. Slors, W. A. Bemelman, F. A. Vyth-Dreese, and A. A. te Velde Dendritic Cell Populations in Colon and Mesenteric Lymph Nodes of Patients With Crohn's Disease J. Histochem. Cytochem., March 1, 2008; 56(3): 233 - 241. [Abstract] [Full Text] [PDF] V. Veckman and I. Julkunen Streptococcus pyogenes activates human plasmacytoid and myeloid dendritic cells J. Leukoc. Biol., February 1, 2008; 83(2): 296 - 304. [Abstract] [Full Text] [PDF] C. Talarn, A. Urbano-Ispizua, R. Martino, J. A. Perez-Simon, M. Batlle, C. Herrera, M. Granell, A. Gaya, M. Torrebadell, F. Fernandez-Aviles, et al. Kinetics of recovery of dendritic cell subsets after reduced-intensity conditioning allogeneic stem cell transplantation and clinical outcome Haematologica, December 1, 2007; 92(12): 1655 - 1663. [Abstract] [Full Text] [PDF] W. J. Mayer, U. M. Irschick, P. Moser, M. Wurm, H. P. Huemer, N. Romani, and E. U. Irschick Characterization of Antigen-Presenting Cells in Fresh and Cultured Human Corneas Using Novel Dendritic Cell Markers Invest. Ophthalmol. Vis. Sci., October 1, 2007; 48(10): 4459 - 4467. [Abstract] [Full Text] [PDF] C. Brignone, C. Grygar, M. Marcu, K. Schakel, and F. Triebel A Soluble Form of Lymphocyte Activation Gene-3 (IMP321) Induces Activation of a Large Range of Human Effector Cytotoxic Cells J. Immunol., September 15, 2007; 179(6): 4202 - 4211. [Abstract] [Full Text] [PDF] A.-C. Lundell, K. Andersson, E. Josefsson, A. Steinkasserer, and A. Rudin Soluble CD14 and CD83 from Human Neonatal Antigen-Presenting Cells Are Inducible by Commensal Bacteria and Suppress Allergen-Induced Human Neonatal Th2 Differentiation Infect. Immun., August 1, 2007; 75(8): 4097 - 4104. [Abstract] [Full Text] [PDF] P. Jain, J. Ahuja, Z. K. Khan, S. Shimizu, O. Meucci, S. R. Jennings, and B. Wigdahl Modulation of dendritic cell maturation and function by the Tax protein of human T cell leukemia virus type 1 J. Leukoc. Biol., July 1, 2007; 82(1): 44 - 56. [Abstract] [Full Text] [PDF] D. Piccioli, S. Tavarini, E. Borgogni, V. Steri, S. Nuti, C. Sammicheli, M. Bardelli, D. Montagna, F. Locatelli, and A. Wack Functional specialization of human circulating CD16 and CD1c myeloid dendritic-cell subsets Blood, June 15, 2007; 109(12): 5371 - 5379. [Abstract] [Full Text] [PDF] K. M. Dhodapkar, D. Banerjee, J. Connolly, A. Kukreja, E. Matayeva, M. C. Veri, J. V. Ravetch, R. M. Steinman, and M. V. Dhodapkar Selective blockade of the inhibitory Fc{gamma} receptor (Fc{gamma}RIIB) in human dendritic cells and monocytes induces a type I interferon response program J. Exp. Med., June 11, 2007; 204(6): 1359 - 1369. [Abstract] [Full Text] [PDF] K. N. Brown, A. Trichel, and S. M. Barratt-Boyes Parallel Loss of Myeloid and Plasmacytoid Dendritic Cells from Blood and Lymphoid Tissue in Simian AIDS J. Immunol., June 1, 2007; 178(11): 6958 - 6967. [Abstract] [Full Text] [PDF] P. U. Cameron, A. J. Handley, D. C. Baylis, A. E. Solomon, N. Bernard, D. F. J. Purcell, and S. R. Lewin Preferential Infection of Dendritic Cells during Human Immunodeficiency Virus Type 1 Infection of Blood Leukocytes J. Virol., March 1, 2007; 81(5): 2297 - 2306. [Abstract] [Full Text] [PDF] L. P. Breitling, R. Fendel, B. Mordmueller, A. A. Adegnika, P. G. Kremsner, and A. J. F. Luty Cord Blood Dendritic Cell Subsets in African Newborns Exposed to Plasmodium falciparum In Utero. Infect. Immun., October 1, 2006; 74(10): 5725 - 5729. [Abstract] [Full Text] [PDF] F. Groot, T. M. M. van Capel, M. L. Kapsenberg, B. Berkhout, and E. C. de Jong Opposing roles of blood myeloid and plasmacytoid dendritic cells in HIV-1 infection of T cells: transmission facilitation versus replication inhibition Blood, September 15, 2006; 108(6): 1957 - 1964. [Abstract] [Full Text] [PDF] I. K. Demedts, K. R. Bracke, T. Maes, G. F. Joos, and G. G. Brusselle Different Roles for Human Lung Dendritic Cell Subsets in Pulmonary Immune Defense Mechanisms Am. J. Respir. Cell Mol. Biol., September 1, 2006; 35(3): 387 - 393. [Abstract] [Full Text] [PDF] C.W. Cutler and R. Jotwani Dendritic Cells at the Oral Mucosal Interface Journal of Dental Research, August 1, 2006; 85(8): 678 - 689. [Abstract] [Full Text] [PDF] J. Abbasian, D. Mahmud, N. Mahmud, S. Chunduri, H. Araki, P. Reddy, R. Hoffman, M. Arpinati, J. L. M. Ferrara, and D. Rondelli Allogeneic T cells induce rapid CD34+ cell differentiation into CD11c+CD86+ cells with direct and indirect antigen-presenting function Blood, July 1, 2006; 108(1): 203 - 208. [Abstract] [Full Text] [PDF] T. M. C. Hornell, T. Burster, F. L. Jahnsen, A. Pashine, M. T. Ochoa, J. J. Harding, C. Macaubas, A. W. Lee, R. L. Modlin, and E. D. Mellins Human Dendritic Cell Expression of HLA-DO Is Subset Specific and Regulated by Maturation J. Immunol., March 15, 2006; 176(6): 3536 - 3547. [Abstract] [Full Text] [PDF] A. Granelli-Piperno, I. Shimeliovich, M. Pack, C. Trumpfheller, and R. M. Steinman HIV-1 Selectively Infects a Subset of Nonmaturing BDCA1-Positive Dendritic Cells in Human Blood J. Immunol., January 15, 2006; 176(2): 991 - 998. [Abstract] [Full Text] [PDF] M. A. Lowes, F. Chamian, M. V. Abello, J. Fuentes-Duculan, S.-L. Lin, R. Nussbaum, I. Novitskaya, H. Carbonaro, I. Cardinale, T. Kikuchi, et al. Increase in TNF-{alpha} and inducible nitric oxide synthase-expressing dendritic cells in psoriasis and reduction with efalizumab (anti-CD11a) PNAS, December 27, 2005; 102(52): 19057 - 19062. [Abstract] [Full Text] [PDF] S. Yamagami, S. Yokoo, T. Usui, H. Yamagami, S. Amano, and N. Ebihara Distinct Populations of Dendritic Cells in the Normal Human Donor Corneal Epithelium Invest. Ophthalmol. Vis. Sci., December 1, 2005; 46(12): 4489 - 4494. [Abstract] [Full Text] [PDF] M. Lindstedt, K. Lundberg, and C. A. K. Borrebaeck Gene Family Clustering Identifies Functionally Associated Subsets of Human In Vivo Blood and Tonsillar Dendritic Cells J. Immunol., October 15, 2005; 175(8): 4839 - 4846. [Abstract] [Full Text] [PDF] B. Morandi, R. Costa, M. Falco, S. Parolini, A. De Maria, G. Ratto, M. C. Mingari, G. Melioli, A. Moretta, and G. Ferlazzo Distinctive Lack of CD48 Expression in Subsets of Human Dendritic Cells Tunes NK Cell Activation J. Immunol., September 15, 2005; 175(6): 3690 - 3697. [Abstract] [Full Text] [PDF] D. McIlroy, S. Tanguy-Royer, N. Le Meur, I. Guisle, P.-J. Royer, J. Leger, K. Meflah, and M. Gregoire Profiling dendritic cell maturation with dedicated microarrays J. Leukoc. Biol., September 1, 2005; 78(3): 794 - 803. [Abstract] [Full Text] [PDF] J. Dalgaard, K. J. Beckstrom, F. L. Jahnsen, and J. E. Brinchmann Differential capability for phagocytosis of apoptotic and necrotic leukemia cells by human peripheral blood dendritic cell subsets J. Leukoc. Biol., May 1, 2005; 77(5): 689 - 698. [Abstract] [Full Text] [PDF] M. Schmitz, S. Zhao, Y. Deuse, K. Schakel, R. Wehner, H. Wohner, K. Holig, F. Wienforth, A. Kiessling, M. Bornhauser, et al. Tumoricidal Potential of Native Blood Dendritic Cells: Direct Tumor Cell Killing and Activation of NK Cell-Mediated Cytotoxicity J. Immunol., April 1, 2005; 174(7): 4127 - 4134. [Abstract] [Full Text] [PDF] S. Vuckovic, D. Khalil, N. Angel, F. Jahnsen, I. Hamilton, A. Boyce, B. Hock, and D. N. J. Hart The CMRF58 antibody recognizes a subset of CD123hi dendritic cells in allergen-challenged mucosa J. Leukoc. Biol., March 1, 2005; 77(3): 344 - 351. [Abstract] [Full Text] [PDF] H. Hashizume, T. Horibe, H. Yagi, N. Seo, and M. Takigawa Compartmental Imbalance and Aberrant Immune Function of Blood CD123+ (Plasmacytoid) and CD11c+ (Myeloid) Dendritic Cells in Atopic Dermatitis J. Immunol., February 15, 2005; 174(4): 2396 - 2403. [Abstract] [Full Text] [PDF] C. M. Smyth, G. Logan, R. Boadle, P. B. Rowe, J. A. Smythe, and I. E. Alexander Differential subcellular localization of CD86 in human PBMC-derived macrophages and DCs, and ultrastructural characterization by immuno-electron microscopy Int. Immunol., February 1, 2005; 17(2): 123 - 132. [Abstract] [Full Text] [PDF] B. A. Zabel, A. M. Silverio, and E. C. Butcher Chemokine-Like Receptor 1 Expression and Chemerin-Directed Chemotaxis Distinguish Plasmacytoid from Myeloid Dendritic Cells in Human Blood J. Immunol., January 1, 2005; 174(1): 244 - 251. [Abstract] [Full Text] [PDF] F. F. Fagnoni, B. Oliviero, G. Giorgiani, P. De Stefano, A. Deho, C. Zibera, N. Gibelli, R. Maccario, G. Da Prada, M. Zecca, et al. Reconstitution dynamics of plasmacytoid and myeloid dendritic cell precursors after allogeneic myeloablative hematopoietic stem cell transplantation Blood, July 1, 2004; 104(1): 281 - 289. [Abstract] [Full Text] [PDF] V. Reddy, J. A. Iturraspe, A. C. Tzolas, H.-U. Meier-Kriesche, J. Schold, and J. R. Wingard Low dendritic cell count after allogeneic hematopoietic stem cell transplantation predicts relapse, death, and acute graft-versus-host disease Blood, June 1, 2004; 103(11): 4330 - 4335. [Abstract] [Full Text] [PDF] H. Fujiwara, F. El Ouriaghli, M. Grube, D. A. Price, K. Rezvani, E. Gostick, G. Sconocchia, J. Melenhorst, N. Hensel, D. C. Douek, et al. Identification and in vitro expansion of CD4+ and CD8+ T cells specific for human neutrophil elastase Blood, April 15, 2004; 103(8): 3076 - 3083. [Abstract] [Full Text] [PDF] D. J. Munster, K. P. A. MacDonald, M. Kato, and D. J. N. Hart Human T lymphoblasts and activated dendritic cells in the allogeneic mixed leukocyte reaction are susceptible to NK cell-mediated anti-CD83-dependent cytotoxicity Int. Immunol., January 1, 2004; 16(1): 33 - 42. [Abstract] [Full Text] [PDF] S. Turville, J. Wilkinson, P. Cameron, J. Dable, and A. L. Cunningham The role of dendritic cell C-type lectin receptors in HIV pathogenesis J. Leukoc. Biol., November 1, 2003; 74(5): 710 - 718. [Abstract] [Full Text] [PDF] N. Teleshova, I. Frank, and M. Pope Immunodeficiency virus exploitation of dendritic cells in the early steps of infection J. Leukoc. Biol., November 1, 2003; 74(5): 683 - 690. [Abstract] [Full Text] [PDF] E. Orsini, A. Guarini, S. Chiaretti, F. R. Mauro, and R. Foa The Circulating Dendritic Cell Compartment in Patients with Chronic Lymphocytic Leukemia Is Severely Defective and Unable to Stimulate an Effective T-Cell Response Cancer Res., August 1, 2003; 63(15): 4497 - 4506. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020