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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Goardon, N. Articles by Maouche-Chrétien, L. Search for Related Content PubMed PubMed Citation Articles by Goardon, N. Articles by Maouche-Chrétien, L. Related Collections Hematopoiesis and Stem Cells Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 July 2002, Vol. 100, No. 2, pp. 491-500 HEMATOPOIESIS Ectopic expression of TAL-1 protein in Ly-6E.1-htal-1 transgenic mice induces defects in B- and T-lymphoid differentiation Nicolas Goardon, Annette Schuh, Iman Hajar, Xiaoqian Ma, Hélène Jouault, Elaine Dzierzak, Paul-Henri Roméo, and Leïla Maouche-Chrétien From the Institut Cochin, Département d'Hématologie, INSERM, CNRS, Paris, France; the Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands; and the Département d'Hématologie, Hôpital Henri Mondor, Créteil, France. The tal-1 gene encodes a basic helix-loop-helix (bHLH) transcription factor required for primitive and definitive hematopoiesis. Additionally, ectopic activation of the tal-1 gene during T lymphopoiesis occurs in numerous cases of human T-cell acute lymphoblastic leukemia. With the use of transgenic mice, we show that, in adult hematopoiesis, constitutive expression of TAL-1 protein causes disorders in the hematopoietic lineages that normally switch off tal-1 gene expression during their differentiation process. Myelopoiesis was characterized by a moderate increase of myeloid precursors and by Sca-1 antigen persistence. Although no lymphoid leukemia was observed, T lymphopoiesis and B lymphopoiesis were severely impaired. Transgenic mice showed reduced thymic cellularity together with a decrease in double-positive cells and a concurrent increase in the single-positive population. B cells exhibited a differentiation defect characterized by a reduction of the B-cell compartment most likely because of a differentiation block upstream of the intermediate pro-B progenitor. B cells escaping this defect developed normally, but transgenic splenocytes presented a defect in immunoglobulin class switch recombination. Altogether, these results enlighten the fine-tuning of TAL-1 expression during adult hematopoiesis and indicate why TAL-1 expression has to be switched off in the lymphoid lineages. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: P. Brunet de la Grange, E. Zink, F. Armstrong, M.-C. Rouyez, and F. Pflumio Impairment of Granulo-Monocytic Development of Human Common Myeloid Progenitors but Not of Granulo-Monocytic Progenitors by Decreasing Stem Cell Leukemia/T-Cell Acute Leukemia 1 Expression Stem Cells, June 1, 2008; 26(6): 1658 - 1662. [Abstract] [Full Text] [PDF] J. R. Gothert, R. L. Brake, M. Smeets, U. Duhrsen, C. G. Begley, and D. J. Izon NOTCH1 pathway activation is an early hallmark of SCL T leukemogenesis Blood, November 15, 2007; 110(10): 3753 - 3762. [Abstract] [Full Text] [PDF] P. B. de la Grange, F. Armstrong, V. Duval, M.-C. Rouyez, N. Goardon, P.-H. Romeo, and F. Pflumio Low SCL/TAL1 expression reveals its major role in adult hematopoietic myeloid progenitors and stem cells Blood, November 1, 2006; 108(9): 2998 - 3004. [Abstract] [Full Text] [PDF] A. Palamarchuk, N. Zanesi, R. I. Aqeilan, A. Efanov, V. Maximov, U. Santanam, J. P. Hagan, C. M. Croce, and Y. Pekarsky Tal1 transgenic expression reveals absence of B lymphocytes. Cancer Res., June 15, 2006; 66(12): 6014 - 6017. [Abstract] [Full Text] [PDF] C. Capron, Y. Lecluse, A. L. Kaushik, A. Foudi, C. Lacout, D. Sekkai, I. Godin, O. Albagli, I. Poullion, F. Svinartchouk, et al. The SCL relative LYL-1 is required for fetal and adult hematopoietic stem cell function and B-cell differentiation Blood, June 15, 2006; 107(12): 4678 - 4686. [Abstract] [Full Text] [PDF] D. Reynaud, E. Ravet, M. Titeux, F. Mazurier, L. Renia, A. Dubart-Kupperschmitt, P.-H. Romeo, and F. Pflumio SCL/TAL1 expression level regulates human hematopoietic stem cell self-renewal and engraftment Blood, October 1, 2005; 106(7): 2318 - 2328. [Abstract] [Full Text] [PDF] M. Tremblay, S. Herblot, E. Lecuyer, and T. Hoang Regulation of pTalpha Gene Expression by a Dosage of E2A, HEB, and SCL J. Biol. Chem., April 4, 2003; 278(15): 12680 - 12687. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020