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Blood, 15 July 2002, Vol. 100, No. 2, pp. 701-703
BRIEF REPORT
MUTZ-3, a human cell line model for the cytokine-induced
differentiation of dendritic cells from CD34+
precursors
Allan J. Masterson,
Claudia
C. Sombroek,
Tanja D. de
Gruijl,
Yvo M. F. Graus,
Hans J. J. van der Vliet,
Sinéad M. Lougheed,
Alfons J. M. van den Eertwegh,
Herbert M. Pinedo, and
Rik J. Scheper
From the Department of Medical Oncology, Division
Immunotherapy, and the Department of Pathology, VU University Medical
Center VUmc, Amsterdam, The Netherlands; and Numico Research BV,
Wageningen, The Netherlands.
Many human myeloid leukemia-derived cell lines possess the ability
to acquire a dendritic cell (DC) phenotype. However, cytokine responsiveness is generally poor, requiring direct manipulation of
intracellular signaling mechanisms for differentiation. In contrast, the CD34+ human acute myeloid leukemia cell line
MUTZ-3 responds to granulocyte macrophage- colony-stimulating
factor (GM-CSF), interleukin 4 (IL-4), and tumor necrosis factor alpha
(TNF ), cytokines known to be pivotal both in vivo and in vitro for
DC generation from monocytes and CD34+ stem cells. In all
respects, MUTZ-3 cells behave as the immortalized equivalent of
CD34+ DC precursors. Upon stimulation with specific
cytokine cocktails, they acquire a phenotype consistent with either
interstitial- or Langerhans-like DCs and upon maturation (mDC), express
CD83. MUTZ-3 DC display the full range of functional antigen processing and presentation pathways. These findings demonstrate the unique suitability of MUTZ-3 cells as an unlimited source of CD34+
DC progenitors for the study of cytokine-induced DC differentiation.
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