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Prepublished online as a Blood First Edition Paper on May 17, 2002; DOI 10.1182/blood-2001-11-0042. This Article Full Text Full Text (PDF) All Versions of this Article: 2001-11-0042v1 100/3/823    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sandrin, V. Articles by Cosset, F.-L. Search for Related Content PubMed PubMed Citation Articles by Sandrin, V. Articles by Cosset, F.-L. Related Collections Gene Therapy Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 August 2002, Vol. 100, No. 3, pp. 823-832 GENE THERAPY Lentiviral vectors pseudotyped with a modified RD114 envelope glycoprotein show increased stability in sera and augmented transduction of primary lymphocytes and CD34+ cells derived from human and nonhuman primates Virginie Sandrin, Bertrand Boson, Patrick Salmon, Wilfried Gay, Didier Nègre, Roger Le Grand, Didier Trono, and François-Loïc Cosset From the Vectorologie Rétrovirale & Thérapie Génique, U412 INSERM, IFR 74, Ecole Normale Supérieure de Lyon, Lyon, France; Department of Genetics and Microbiology, Faculty of Medicine, University of Geneva, Geneva, Switzerland; and CEA, Service de Neurologie, CRSSA, Fontenay aux Roses, France. Generating lentiviral vectors pseudotyped with different viral glycoproteins (GPs) may modulate the physicochemical properties of the vectors, their interaction with the host immune system, and their host range. We have investigated the capacity of a panel of GPs of both retroviral (amphotropic murine leukemia virus [MLV-A]; gibbon ape leukemia virus [GALV]; RD114, feline endogenous virus) and nonretroviral (fowl plague virus [FPV]; Ebola virus [EboV]; vesicular stomatitis virus [VSV]; lymphocytic choriomeningitis virus [LCMV]) origins to pseudotype lentiviral vectors derived from simian immunodeficiency virus (SIVmac251). SIV vectors were efficiently pseudotyped with the FPV hemagglutinin, VSV-G, LCMV, and MLV-A GPs. In contrast, the GALV and RD114 GPs conferred much lower infectivity to the vectors. Capitalizing on the conservation of some structural features in the transmembrane domains and cytoplasmic tails of the incorporation-competent MLV-A GP and in RD114 and GALV GPs, we generated chimeric GPs encoding the extracellular and transmembrane domains of GALV or RD114 GPs fused to the cytoplasmic tail (designated TR) of MLV-A GP. Importantly, SIV-derived vectors pseudotyped with these GALV/TR and RD114/TR GP chimeras had significantly higher titers than vectors coated with the parental GPs. Additionally, RD114/TR-pseudotyped vectors were efficiently concentrated and were resistant to inactivation induced by the complement of both human and macaque sera, indicating that modified RD114 GP-pseudotyped lentiviral vectors may be of particular interest for in vivo gene transfer applications. Furthermore, as compared to vectors pseudotyped with other retroviral GPs or with VSV-G, RD114/TR-pseudotyped vectors showed augmented transduction of human and macaque primary blood lymphocytes and CD34+ cells. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Dreux, B. Boson, S. Ricard-Blum, J. Molle, D. Lavillette, B. Bartosch, E.-I. Pecheur, and F.-L. Cosset The Exchangeable Apolipoprotein ApoC-I Promotes Membrane Fusion of Hepatitis C Virus J. Biol. Chem., November 2, 2007; 282(44): 32357 - 32369. [Abstract] [Full Text] [PDF] Y.-W. Pan, J. M. Scarlett, T. T. Luoh, and P. Kurre Prolonged Adherence of Human Immunodeficiency Virus-Derived Vector Particles to Hematopoietic Target Cells Leads to Secondary Transduction In Vitro and In Vivo J. Virol., January 15, 2007; 81(2): 639 - 649. [Abstract] [Full Text] [PDF] F. Helle, C. Wychowski, N. Vu-Dac, K. R. 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	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020