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Prepublished online as a Blood First Edition Paper on April 30, 2002; DOI 10.1182/blood-2002-02-0408. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-02-0408v1 100/3/961    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chiriva-Internati, M. Articles by Lim, S. H. Search for Related Content PubMed PubMed Citation Articles by Chiriva-Internati, M. Articles by Lim, S. H. Related Collections Neoplasia Immunotherapy Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 August 2002, Vol. 100, No. 3, pp. 961-965 NEOPLASIA Sperm protein 17 (Sp17) is a suitable target for immunotherapy of multiple myeloma Maurizio Chiriva-Internati, Zhiqing Wang, Emanuela Salati, Klaus Bumm, Bart Barlogie, and Seah H. Lim From the Division of Hematology and Oncology, Texas Tech University Health Sciences Center, Amarillo; Blood Stem Cell Transplantation Program, Don and Sybil Harrington Cancer Center, Amarillo, TX, and Myeloma and Transplantation Research Center, University of Arkansas for Medical Sciences, Little Rock, AR. Sperm protein 17 (Sp17) is a protein recently identified as a novel cancer-testis (CT) antigen in multiple myeloma (MM). Because this tumor antigen demonstrates a very restricted normal tissue expression, Sp17 may be an excellent target for tumor vaccine of MM. In this study, we determined the ability to generate Sp17-specific HLA class I-restricted cytotoxic T lymphocytes (CTLs) from the peripheral blood of 4 patients with MM, 3 consecutive Sp17+ patients, and 1 Sp17 patient. Dendritic cells were generated from monocytes of 4 patients with MM and used to present a recombinant Sp17 protein to autologous T cells. Following 4 rounds of antigen stimulation, the CTLs were tested for their ability to kill autologous targets in an Sp17-dependent and HLA-class I- restricted manner in standard cytotoxicity assays. Despite previous chemotherapy and the immunosuppression so often associated with MM, CTL generation was successful in all 4 patients, irrespective of the Sp17 status of their tumors. Most importantly, the CTLs were able to lyse autologous tumor cells that expressed Sp17. Tumor cell lysis in all cases appeared to be mainly mediated by perforin and could be blocked by concanamycin A. We conclude that Sp17 is a suitable target for immunotherapy of MM. Our findings provide the basis for a clinical study aimed at inducing a cellular immune response directed at Sp17+ MM. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: W. J. Chng, S. Kumar, S. VanWier, G. Ahmann, T. Price-Troska, K. Henderson, T.-H. Chung, S. Kim, G. Mulligan, B. Bryant, et al. Molecular Dissection of Hyperdiploid Multiple Myeloma by Gene Expression Profiling Cancer Res., April 1, 2007; 67(7): 2982 - 2989. [Abstract] [Full Text] [PDF] M. Condomines, D. Hose, P. Raynaud, M. Hundemer, J. De Vos, M. Baudard, T. Moehler, V. Pantesco, M. Moos, J.-F. Schved, et al. 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Cancer Res., November 1, 2004; 10(21): 7402 - 7412. [Abstract] [Full Text] [PDF] F. Grizzi, M. Chiriva-Internati, B. Franceschini, K. Bumm, P. Colombo, M. Ciccarelli, E. Donetti, N. Gagliano, P. L. Hermonat, R. K. Bright, et al. Sperm Protein 17 Is Expressed in Human Somatic Ciliated Epithelia J. Histochem. Cytochem., April 1, 2004; 52(4): 549 - 554. [Abstract] [Full Text] [PDF] M. Chiriva-Internati, F. Grizzi, B. Franceschini, P. L. Hermonat, R. K. Bright, K. Bumm, N. Dioguardi, and W. M. Kast Is sperm protein 17 a useful target for tumor immunotherapy? Blood, September 15, 2003; 102(6): 2308 - 2309. [Full Text] [PDF] B. Zhu, Z. Chen, X. Cheng, Z. Lin, J. Guo, Z. Jia, L. Zou, Z. Wang, Y. Hu, D. Wang, et al. Identification of HLA-A*0201-restricted Cytotoxic T Lymphocyte Epitope from TRAG-3 Antigen Clin. Cancer Res., May 1, 2003; 9(5): 1850 - 1857. [Abstract] [Full Text] [PDF] Z. Wang, Y. Zhang, H. Liu, E. Salati, M. Chiriva-Internati, and S. H. 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