|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 August 2002, Vol. 100, No. 4, pp. 1123-1132
PLENARY PAPER
Development of myelofibrosis in mice genetically impaired for
GATA-1 expression (GATA-1low mice)
Alessandro Maria Vannucchi,
Lucia Bianchi,
Cristina Cellai,
Francesco Paoletti,
Rosa Alba Rana,
Rodolfo Lorenzini,
Giovanni Migliaccio, and
Anna Rita Migliaccio
From the Departments of Ematologia and
Oncologia-Patologia Sperimentale, University of Florence; Azienda
Ospedaliera Careggi, Florence; Department of Biomorfologia, University
G. D'Annunzio, Chieti; and Servizio Qualità Sicurezza Animale,
Biologia Cellulare, and Biochimica Clinica, Istituto Superiore
Sanità, Rome, Italy.
The phenotype induced by the GATA-1low (neo HS)
mutation is here further characterized by analyzing the hemopoietic
system during the aging (up to 20 months) of a GATA-1low
colony (135 mutants and 40 normal littermates). Mutants expressed normal hematocrit values (Hct = 45.9 ± 4.0) until 12 months but became anemic from 15 months on (Hct = 30.9 ± 3.9;
P < .05). Anemia was associated with several markers of
myelofibrosis such as the presence of tear-drop poikilocytes and
progenitor cells in the blood, collagen fibers in the marrow and in the
spleen, and hemopoietic foci in the liver. Semiquantitative reverse
transcription-polymerase chain reaction showed that growth factor
genes implicated in the development of myelofibrosis (such as
osteocalcin, transforming growth factor- 1, platelet-derived growth
factor, and vascular endothelial growth factor) were all expressed in
the marrow from the mutants at higher levels than in corresponding
normal tissues. The GATA-1low mutants experienced a slow
progression of the disease because the final exitus was not observed
until at least 15 months with a probability of survival more favorable
than that of W/Wv mice concurrently kept in the animal
facility (P < .001, by Kaplan-Meier analysis). In
conclusion, impaired GATA-1 expression may contribute to the
development of myelofibrosis, and the GATA-1low mutants may
represent a suitable animal model for the human disease that may shed
light on its pathogenesis.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
Related Letter in Blood Online:
-
Lack of alteration in GATA-1 expression in CD34+ hematopoietic progenitors from patients with idiopathic myelofibrosis
- Marie-Claire Martyré, Virginie Steunou, Marie-Caroline LeBousse-Kerdilès, Juana Wietzerbin, Alessandro Maria Vannucchi, and Anna Rita Migliaccio
Blood 2003 101: 5087-5089.
[Full Text]
[PDF]
This article has been cited by other articles:
|
|
|
|
 
R. L. Levine and D. G. Gilliland
Myeloproliferative disorders
Blood,
September 15, 2008;
112(6):
2190 - 2198.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Lorenzo, M. Horowitz, and Y. Choi
Osteoimmunology: Interactions of the Bone and Immune System
Endocr. Rev.,
June 1, 2008;
29(4):
403 - 440.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Bock, J. Hoftmann, K. Theophile, K. Hussein, B. Wiese, J. Schlue, and H. Kreipe
Bone Morphogenetic Proteins Are Overexpressed in the Bone Marrow of Primary Myelofibrosis and Are Apparently Induced by Fibrogenic Cytokines
Am. J. Pathol.,
April 1, 2008;
172(4):
951 - 960.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Garimella, M. A. Kacena, S. E. Tague, J. Wang, M. C. Horowitz, and H. C. Anderson
Expression of Bone Morphogenetic Proteins and Their Receptors in the Bone Marrow Megakaryocytes of GATA-1low Mice: A Possible Role in Osteosclerosis
J. Histochem. Cytochem.,
July 1, 2007;
55(7):
745 - 752.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Wagner-Ballon, D. F. Pisani, T. Gastinne, M. Tulliez, R. Chaligne, C. Lacout, F. Aurade, J.-L. Villeval, P. Gonin, W. Vainchenker, et al.
Proteasome inhibitor bortezomib impairs both myelofibrosis and osteosclerosis induced by high thrombopoietin levels in mice
Blood,
July 1, 2007;
110(1):
345 - 353.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Tefferi
Primary myelofibrosis and its paraneoplastic stromal effects
Haematologica,
May 1, 2007;
92(5):
577 - 579.
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Zetterberg, A. M. Vannucchi, A. R. Migliaccio, W. Vainchenker, M. Tulliez, R. Dickie, H. Hasselbalch, R. Rogers, and J. Palmblad
Pericyte coverage of abnormal blood vessels in myelofibrotic bone marrows
Haematologica,
May 1, 2007;
92(5):
597 - 604.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Crispino
On the origins of megakaryocytes
Blood,
February 15, 2007;
109(4):
1340 - 1341.
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Ghinassi, M. Sanchez, F. Martelli, G. Amabile, A. M. Vannucchi, G. Migliaccio, S. H. Orkin, and A. R. Migliaccio
The hypomorphic Gata1low mutation alters the proliferation/differentiation potential of the common megakaryocytic-erythroid progenitor
Blood,
February 15, 2007;
109(4):
1460 - 1471.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Guglielmelli, R. Zini, C. Bogani, S. Salati, A. Pancrazzi, E. Bianchi, F. Mannelli, S. Ferrari, M.-C. Le Bousse-Kerdiles, A. Bosi, et al.
Molecular Profiling of CD34+ Cells in Idiopathic Myelofibrosis Identifies a Set of Disease-Associated Genes and Reveals the Clinical Significance of Wilms' Tumor Gene 1 (WT1)
Stem Cells,
January 1, 2007;
25(1):
165 - 173.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Lacout, D. F. Pisani, M. Tulliez, F. M. Gachelin, W. Vainchenker, and J.-L. Villeval
JAK2V617F expression in murine hematopoietic cells leads to MPD mimicking human PV with secondary myelofibrosis
Blood,
September 1, 2006;
108(5):
1652 - 1660.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Tefferi, G. Barosi, R. A. Mesa, F. Cervantes, H. J. Deeg, J. T. Reilly, S. Verstovsek, B. Dupriez, R. T. Silver, O. Odenike, et al.
International Working Group (IWG) consensus criteria for treatment response in myelofibrosis with myeloid metaplasia, for the IWG for Myelofibrosis Research and Treatment (IWG-MRT)
Blood,
September 1, 2006;
108(5):
1497 - 1503.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Wagner-Ballon, H. Chagraoui, E. Prina, M. Tulliez, G. Milon, H. Raslova, J.-L. Villeval, W. Vainchenker, and S. Giraudier
Monocyte/Macrophage Dysfunctions Do Not Impair the Promotion of Myelofibrosis by High Levels of Thrombopoietin.
J. Immunol.,
June 1, 2006;
176(11):
6425 - 6433.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. P. Steensma, A. Pardanani, W. S. Stevenson, R. Hoyt, H. Kiu, A. P. Grigg, J. Szer, S. Juneja, D. J. Hilton, W. S. Alexander, et al.
More on Myb in myelofibrosis: molecular analyses of MYB and EP300 in 55 patients with myeloproliferative disorders
Blood,
February 15, 2006;
107(4):
1733 - 1735.
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. L. Stachura, S. T. Chou, and M. J. Weiss
Early block to erythromegakaryocytic development conferred by loss of transcription factor GATA-1
Blood,
January 1, 2006;
107(1):
87 - 97.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Martelli, B. Ghinassi, B. Panetta, E. Alfani, V. Gatta, A. Pancrazzi, C. Bogani, A. M. Vannucchi, F. Paoletti, G. Migliaccio, et al.
Variegation of the phenotype induced by the Gata1low mutation in mice of different genetic backgrounds
Blood,
December 15, 2005;
106(13):
4102 - 4113.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Tefferi
Pathogenesis of Myelofibrosis With Myeloid Metaplasia
J. Clin. Oncol.,
November 20, 2005;
23(33):
8520 - 8530.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Vannucchi, A. Pancrazzi, P. Guglielmelli, S. Di Lollo, C. Bogani, G. Baroni, L. Bianchi, A. R. Migliaccio, A. Bosi, and F. Paoletti
Abnormalities of GATA-1 in Megakaryocytes from Patients with Idiopathic Myelofibrosis
Am. J. Pathol.,
September 1, 2005;
167(3):
849 - 858.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Munugalavadla, L. C. Dore, B. L. Tan, L. Hong, M. Vishnu, M. J. Weiss, and R. Kapur
Repression of c-Kit and Its Downstream Substrates by GATA-1 Inhibits Cell Proliferation during Erythroid Maturation
Mol. Cell. Biol.,
August 1, 2005;
25(15):
6747 - 6759.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. C. Jones, A. Tefferi, P. T. Vuong, J. C. Desmond, W.-K. Hofmann, and H. P. Koeffler
Detection of Aberrant Gene Expression in CD34+ Hematopoietic Stem Cells from Patients with Agnogenic Myeloid Metaplasia Using Oligonucleotide Microarrays
Stem Cells,
May 1, 2005;
23(5):
631 - 637.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Vannucchi, L. Bianchi, F. Paoletti, A. Pancrazzi, E. Torre, M. Nishikawa, M. Zingariello, A. Di Baldassarre, R. A. Rana, R. Lorenzini, et al.
A pathobiologic pathway linking thrombopoietin, GATA-1, and TGF-{beta}1 in the development of myelofibrosis
Blood,
May 1, 2005;
105(9):
3493 - 3501.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Komura, C. Tonetti, V. Penard-Lacronique, H. Chagraoui, C. Lacout, J. P. LeCouedic, P. Rameau, N. Debili, W. Vainchenker, and S. Giraudier
Role for the Nuclear Factor {kappa}B Pathway in Transforming Growth Factor-{beta}1 Production in Idiopathic Myelofibrosis: Possible Relationship with FK506 Binding Protein 51 Overexpression
Cancer Res.,
April 15, 2005;
65(8):
3281 - 3289.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Toki, F. Katsuoka, R. Kanezaki, G. Xu, H. Kurotaki, J. Sun, T. Kamio, S. Watanabe, S. Tandai, K. Terui, et al.
Transgenic expression of BACH1 transcription factor results in megakaryocytic impairment
Blood,
April 15, 2005;
105(8):
3100 - 3108.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Centurione, A. Di Baldassarre, M. Zingariello, D. Bosco, V. Gatta, R. A. Rana, V. Langella, A. Di Virgilio, A. M. Vannucchi, and A. R. Migliaccio
Increased and pathologic emperipolesis of neutrophils within megakaryocytes associated with marrow fibrosis in GATA-1low mice
Blood,
December 1, 2004;
104(12):
3573 - 3580.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. R. Migliaccio, F. Martelli, B. Panetta, E. Alfani, G. Migliaccio, V. Gatta, A. Di Baldassarre, A. Pancrazi, C. Bogani, F. Paoletti, et al.
Effects of the Genetic Background on the Myelofibrosis Induced by the GATA-1Low Mutation.
Blood (ASH Annual Meeting Abstracts),
November 16, 2004;
104(11):
2433 - 2433.
[Abstract]
|
|
|
|
|
|
|
P. Francia, C. delli Gatti, M. Bachschmid, I. Martin-Padura, C. Savoia, E. Migliaccio, P. G. Pelicci, M. Schiavoni, T. F. Luscher, M. Volpe, et al.
Deletion of p66shc Gene Protects Against Age-Related Endothelial Dysfunction
Circulation,
November 2, 2004;
110(18):
2889 - 2895.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Gurbuxani, P. Vyas, and J. D. Crispino
Recent insights into the mechanisms of myeloid leukemogenesis in Down syndrome
Blood,
January 15, 2004;
103(2):
399 - 406.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. O'Brien, A. Tefferi, and P. Valent
Chronic Myelogenous Leukemia and Myeloproliferative Disease
Hematology,
January 1, 2004;
2004(1):
146 - 162.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-C. Martyre, V. Steunou, M.-C. LeBousse-Kerdiles, J. Wietzerbin, A. M. Vannucchi, and A. R. Migliaccio
Lack of alteration in GATA-1 expression in CD34+ hematopoietic progenitors from patients with idiopathic myelofibrosis
Blood,
June 15, 2003;
101(12):
5087 - 5089.
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Tefferi
The Forgotten Myeloproliferative Disorder: Myeloid Metaplasia
Oncologist,
June 1, 2003;
8(3):
225 - 231.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Chagraoui, M. Tulliez, T. Smayra, E. Komura, S. Giraudier, T. Yun, N. Lassau, W. Vainchenker, and F. Wendling
Stimulation of osteoprotegerin production is responsible for osteosclerosis in mice overexpressing TPO
Blood,
April 15, 2003;
101(8):
2983 - 2989.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. R. Migliaccio, R. A. Rana, M. Sanchez, R. Lorenzini, L. Centurione, L. Bianchi, A. M. Vannucchi, G. Migliaccio, and S. H. Orkin
GATA-1 as a Regulator of Mast Cell Differentiation Revealed by the Phenotype of the GATA-1low Mouse Mutant
J. Exp. Med.,
February 3, 2003;
197(3):
281 - 296.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Spivak, G. Barosi, G. Tognoni, T. Barbui, G. Finazzi, R. Marchioli, and M. Marchetti
Chronic Myeloproliferative Disorders
Hematology,
January 1, 2003;
2003(1):
200 - 224.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
