|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 August 2002, Vol. 100, No. 4, pp. 1502-1504
BRIEF REPORT
Native human blood dendritic cells as potent effectors in
antibody-dependent cellular cytotoxicity
Marc Schmitz,
Senming Zhao,
Knut Schäkel,
Martin Bornhäuser,
Detlef Ockert, and
Ernst Peter Rieber
From the Institute of Immunology, Department of
Medicine I, and Department of Surgery, Medical Faculty, Technical
University of Dresden, Germany.
Functional studies on native human dendritic cells (DCs) are
hampered by technical difficulties in preparing fresh DCs. Recently, with the help of the monoclonal antibody M-DC8, we succeeded in isolating a major subpopulation of human blood DCs by a one-step immunomagnetic separation procedure. These cells strongly express Fc RIII (CD16) and FcRII (CD32) and are quite efficient in the antigen-specific activation of naive T cells. Because some Fc receptor-bearing cell types are known as effector cells in
antibody-dependent cellular cytotoxicity (ADCC), we investigated
whether M-DC8+ DCs are capable of effectuating ADCC. In
this report we show that freshly prepared M-DC8+ DCs
efficiently mediate tumor-directed ADCC and that both types of Fc
receptors as well as tumor necrosis factor  essentially contribute
to the cytotoxic activity. The results provide evidence that, in
addition to their pivotal role in primary T-cell activation, a subset
of blood DCs displays efficient cytotoxicity in ADCC.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
Z. M. Bamboat, J. A. Stableford, G. Plitas, B. M. Burt, H. M. Nguyen, A. P. Welles, M. Gonen, J. W. Young, and R. P. DeMatteo
Human Liver Dendritic Cells Promote T Cell Hyporesponsiveness
J. Immunol.,
February 15, 2009;
182(4):
1901 - 1911.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Piccioli, S. Tavarini, E. Borgogni, V. Steri, S. Nuti, C. Sammicheli, M. Bardelli, D. Montagna, F. Locatelli, and A. Wack
Functional specialization of human circulating CD16 and CD1c myeloid dendritic-cell subsets
Blood,
June 15, 2007;
109(12):
5371 - 5379.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Holl, M. Peressin, S. Schmidt, T. Decoville, S. Zolla-Pazner, A.-M. Aubertin, and C. Moog
Efficient inhibition of HIV-1 replication in human immature monocyte-derived dendritic cells by purified anti-HIV-1 IgG without induction of maturation
Blood,
June 1, 2006;
107(11):
4466 - 4474.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Bayry, S. Lacroix-Desmazes, M. D. Kazatchkine, O. Hermine, D. F. Tough, and S. V. Kaveri
Modulation of Dendritic Cell Maturation and Function by B Lymphocytes
J. Immunol.,
July 1, 2005;
175(1):
15 - 20.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Schmitz, S. Zhao, Y. Deuse, K. Schakel, R. Wehner, H. Wohner, K. Holig, F. Wienforth, A. Kiessling, M. Bornhauser, et al.
Tumoricidal Potential of Native Blood Dendritic Cells: Direct Tumor Cell Killing and Activation of NK Cell-Mediated Cytotoxicity
J. Immunol.,
April 1, 2005;
174(7):
4127 - 4134.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Schmitz, S. Zhao, Y. Deuse, K. Schaekel, M. Bornhaeuser, and P. Rieber
Tumoricidal Potential of Native Human Blood Dendritic Cells: Direct Tumor Cell Killing and Activation of NK Cell-Mediated Cytotoxicity.
Blood (ASH Annual Meeting Abstracts),
November 16, 2004;
104(11):
449 - 449.
[Abstract]
|
|
|
|
|
|
|
F. F. Fagnoni, B. Oliviero, G. Giorgiani, P. De Stefano, A. Deho, C. Zibera, N. Gibelli, R. Maccario, G. Da Prada, M. Zecca, et al.
Reconstitution dynamics of plasmacytoid and myeloid dendritic cell precursors after allogeneic myeloablative hematopoietic stem cell transplantation
Blood,
July 1, 2004;
104(1):
281 - 289.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
