|
|
 Previous Article | Table of Contents | Next Article 
Blood, 1 September 2002, Vol. 100, No. 5, pp. 1795-1801
NEOPLASIA
Protection of CLL B cells by a follicular dendritic cell line is
dependent on induction of Mcl-1
Irene M. Pedersen,
Shinichi Kitada,
Lorenzo M. Leoni,
Juan M. Zapata,
James G. Karras,
Nobuhiro Tsukada,
Thomas J. Kipps,
Yong Sung Choi,
Frank Bennett, and
John C. Reed
From The Burnham Institute, and University of
California San Diego, Dept Medicine, La Jolla, CA; Laboratory
of Cellular Immunology, Ochsner Foundation, New Orleans, LA; and Isis
Pharmaceuticals, Carlsbad, CA.
Chronic lymphocytic leukemia (CLL) B cells have defects in
apoptosis pathways and therefore accumulate in vivo. However, when removed from the patient and cultured in vitro, these malignant cells
rapidly undergo apoptosis. Recent studies suggest that leukemia cell
survival is influenced by interactions with nonleukemia cells in the
microenvironment of lymph nodes, marrow, and other tissues. To model
such cell-cell interactions in vitro, we cultured freshly isolated CLL
B cells with a follicular dendritic cell line, HK. CLL B cells
cocultured with HK cells were protected from apoptosis, either
spontaneous or induced by treatment with anticancer drugs. Protection
against spontaneous apoptosis could also be induced by coculturing the
CLL B cells with normal dendritic cells (DCs) or with a CD40-ligand
(CD154)-expressing fibroblast cell line. Examination of the expression
of several apoptosis-regulatory proteins revealed that coculture with
HK cells or DCs induced up-regulation of the antiapoptotic Bcl-2 family
protein Mcl-1 in CLL B cells, whereas CD40 ligation increased
expression of Bcl-XL. Cell-cell contact was required for
HK-induced protection, and introducing neutralizing antibodies against
various adhesion molecules showed that CD44 was involved in HK-mediated
survival, whereas CD40, intercellular adhesion molecule-1
(ICAM-1) and vascular cell adhesion molecule-1
(VCAM-1) were not. Anti-CD44 antibodies also blocked Mcl-1
induction by HK cells. Mcl-1 antisense oligonucleotides reduced
leukemia cell expression of Mcl-1, and significantly suppressed HK-induced protection against apoptosis, whereas control
oligonucleotides had no effect. Thus, HK cells protect CLL B cells
against apoptosis, at least in part through a CD44-dependent mechanism
involving up-regulation of Mcl-1, and this mechanism is distinct from
that achieved by CD40 ligation. Consequently, the particular
antiapoptotic proteins important for CLL survival may vary depending on
the microenvironment.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
T. Fukuda, L. Chen, T. Endo, L. Tang, D. Lu, J. E. Castro, G. F. Widhopf II, L. Z. Rassenti, M. J. Cantwell, C. E. Prussak, et al.
Antisera induced by infusions of autologous Ad-CD154-leukemia B cells identify ROR1 as an oncofetal antigen and receptor for Wnt5a
PNAS,
February 26, 2008;
105(8):
3047 - 3052.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. I. Pathan, P. Chu, K. Hariharan, C. Cheney, A. Molina, and J. Byrd
Mediation of apoptosis by and antitumor activity of lumiliximab in chronic lymphocytic leukemia cells and CD23+ lymphoma cell lines
Blood,
February 1, 2008;
111(3):
1594 - 1602.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. G. Longo, L. Laurenti, S. Gobessi, S. Sica, G. Leone, and D. G. Efremov
The Akt/Mcl-1 pathway plays a prominent role in mediating antiapoptotic signals downstream of the B-cell receptor in chronic lymphocytic leukemia B cells
Blood,
January 15, 2008;
111(2):
846 - 855.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Burkle, M. Niedermeier, A. Schmitt-Graff, W. G. Wierda, M. J. Keating, and J. A. Burger
Overexpression of the CXCR5 chemokine receptor, and its ligand, CXCL13 in B-cell chronic lymphocytic leukemia
Blood,
November 1, 2007;
110(9):
3316 - 3325.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. P. Kater, M. H. J. van Oers, and T. J. Kipps
Cellular immune therapy for chronic lymphocytic leukemia
Blood,
October 15, 2007;
110(8):
2811 - 2818.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Willimott, M. Baou, S. Huf, S. Deaglio, and S. D. Wagner
Regulation of CD38 in proliferating chronic lymphocytic leukemia cells stimulated with CD154 and interleukin-4
Haematologica,
October 1, 2007;
92(10):
1359 - 1366.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. G. Mueller, C. Boix, W.-H. Kwan, C. Daussy, E. Fournier, W. H. Fridman, and T. J. Molina
Critical role of monocytes to support normal B cell and diffuse large B cell lymphoma survival and proliferation
J. Leukoc. Biol.,
September 1, 2007;
82(3):
567 - 575.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Smit, D. Y.H. Hallaert, R. Spijker, B. de Goeij, A. Jaspers, A. P. Kater, M. H.J. van Oers, C. J.M. van Noesel, and E. Eldering
Differential Noxa/Mcl-1 balance in peripheral versus lymph node chronic lymphocytic leukemia cells correlates with survival capacity
Blood,
February 15, 2007;
109(4):
1660 - 1668.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ruiz, Y. Krupnik, M. Keating, J. Chandra, M. Palladino, and D. McConkey
The proteasome inhibitor NPI-0052 is a more effective inducer of apoptosis than bortezomib in lymphocytes from patients with chronic lymphocytic leukemia.
Mol. Cancer Ther.,
July 1, 2006;
5(7):
1836 - 1843.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Klener, M. Szynal, Y. Cleuter, M. Merimi, H. Duvillier, F. Lallemand, C. Bagnis, P. Griebel, C. Sotiriou, A. Burny, et al.
Insights into Gene Expression Changes Impacting B-Cell Transformation: Cross-Species Microarray Analysis of Bovine Leukemia Virus Tax-Responsive Genes in Ovine B Cells
J. Virol.,
February 15, 2006;
80(4):
1922 - 1938.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Carew, S. T. Nawrocki, Y. V. Krupnik, K. Dunner Jr, D. J. McConkey, M. J. Keating, and P. Huang
Targeting endoplasmic reticulum protein transport: a novel strategy to kill malignant B cells and overcome fludarabine resistance in CLL
Blood,
January 1, 2006;
107(1):
222 - 231.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Burkle and J. A. Burger
Overexpression of the CXCR5 Chemokine Receptor, and Its Ligand, B Cell-Activating Chemokine-1 (BCA-1/CXCL13) in B Cell Chronic Lymphocytic Leukemia.
Blood (ASH Annual Meeting Abstracts),
November 16, 2005;
106(11):
2948 - 2948.
[Abstract]
|
|
|
|
|
|
|
K. Balakrishnan, W. G. Wierda, M. J. Keating, and V. Gandhi
Mechanisms of Cell Death of Chronic Lymphocytic Leukemia Lymphocytes by RNA-Directed Agent, 8-NH2-Adenosine
Clin. Cancer Res.,
September 15, 2005;
11(18):
6745 - 6752.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Nishio, T. Endo, N. Tsukada, J. Ohata, S. Kitada, J. C. Reed, N. J. Zvaifler, and T. J. Kipps
Nurselike cells express BAFF and APRIL, which can promote survival of chronic lymphocytic leukemia cells via a paracrine pathway distinct from that of SDF-1{alpha}
Blood,
August 1, 2005;
106(3):
1012 - 1020.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. E. Battle, J. Arbiser, and D. A. Frank
The natural product honokiol induces caspase-dependent apoptosis in B-cell chronic lymphocytic leukemia (B-CLL) cells
Blood,
July 15, 2005;
106(2):
690 - 697.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Petlickovski, L. Laurenti, X. Li, S. Marietti, P. Chiusolo, S. Sica, G. Leone, and D. G. Efremov
Sustained signaling through the B-cell receptor induces Mcl-1 and promotes survival of chronic lymphocytic leukemia B cells
Blood,
June 15, 2005;
105(12):
4820 - 4827.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Dicker, A. P. Kater, T. Fukuda, and T. J. Kipps
Fas-ligand (CD178) and TRAIL synergistically induce apoptosis of CD40-activated chronic lymphocytic leukemia B cells
Blood,
April 15, 2005;
105(8):
3193 - 3198.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Hu, N. Haney, D. Kropp, A. F. Kabore, J. B. Johnston, and S. B. Gibson
Lysophosphatidic Acid (LPA) Protects Primary Chronic Lymphocytic Leukemia Cells from Apoptosis through LPA Receptor Activation of the Anti-apoptotic Protein AKT/PKB
J. Biol. Chem.,
March 11, 2005;
280(10):
9498 - 9508.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Chiorazzi, K. R. Rai, and M. Ferrarini
Chronic Lymphocytic Leukemia
N. Engl. J. Med.,
February 24, 2005;
352(8):
804 - 815.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. K. Lee, N. D. Bone, A. K. Strege, T. D. Shanafelt, D. F. Jelinek, and N. E. Kay
VEGF receptor phosphorylation status and apoptosis is modulated by a green tea component, epigallocatechin-3-gallate (EGCG), in B-cell chronic lymphocytic leukemia
Blood,
August 1, 2004;
104(3):
788 - 794.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. K. Stevenson and F. Caligaris-Cappio
Chronic lymphocytic leukemia: revelations from the B-cell receptor
Blood,
June 15, 2004;
103(12):
4389 - 4395.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kitada and J. C. Reed
MCL-1 Promoter Insertions Dial-Up Aggressiveness of Chronic Leukemia
J Natl Cancer Inst,
May 5, 2004;
96(9):
642 - 643.
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. W. C. J. van de Donk, D. Schotte, M. M. J. Kamphuis, A. M. W. van Marion, B. van Kessel, A. C. Bloem, and H. M. Lokhorst
Protein Geranylgeranylation Is Critical for the Regulation of Survival and Proliferation of Lymphoma Tumor Cells
Clin. Cancer Res.,
November 15, 2003;
9(15):
5735 - 5748.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. Frankel, D. R. Fleming, P. D. Hall, B. L. Powell, J. H. Black, C. Leftwich, and R. Gartenhaus
A Phase II Study of DT Fusion Protein Denileukin Diftitox in Patients with Fludarabine-refractory Chronic Lymphocytic Leukemia
Clin. Cancer Res.,
September 1, 2003;
9(10):
3555 - 3561.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
