|
|
 Previous Article | Table of Contents | Next Article 
Blood, 1 September 2002, Vol. 100, No. 5, pp. 1852-1859
PHAGOCYTES
SHP-1 regulates Fc receptor-mediated phagocytosis and the
activation of RAC
Anita M. Kant,
Pradip De,
Xiaodong Peng,
Taolin Yi,
David J. Rawlings,
Jong Suk Kim, and
Donald L. Durden
From the Herman B. Wells Center for Pediatrics
Research, Department of Pediatrics, Biochemistry and Molecular Biology,
Indiana University School of Medicine, Indianapolis; the Childrens
Hospital Los Angeles Research Institute, University of Southern
California School of Medicine, Los Angeles; the Department of
Pediatrics, Division of Immunology/Rheumatology, University of
Washington School of Medicine, Seattle; and the Department of Cancer
Biology, Cleveland Clinic Foundation Research Institute, OH.
Fc receptor-mediated phagocytosis is a complex process
involving the activation of protein tyrosine kinases, events that are
potentially down-regulated by protein tyrosine phosphatases. We used
the J774A.1 macrophage cell line to examine the roles played by the
protein tyrosine phosphatase SHP-1 in the negative regulation of Fc
receptor-mediated phagocytosis. Stimulation with sensitized sheep red
blood cells (sRBCs) induced tyrosine phosphorylation of CBL and
association of CBL with CRKL. These events were completely or partially
abrogated by PP1 or the heterologous expression of dominant-negative
SYK, respectively. Heterologous expression of wild-type but not
catalytically inactive SHP-1 also completely abrogated the phagocytosis
of IgG-sensitized sRBCs. Most notably, overexpressed SHP-1 associates
with CBL and this association led to CBL dephosphorylation, loss of the
CBL-CRKL interaction, and the suppression of Rac activation. These data represent the first direct evidence that SHP-1 is involved in the
regulation of Fc receptor-mediated phagocytosis and suggest that
activating signals mediated by SRC family kinases SYK, CBL, phosphatidyl inositol-3 (PI-3) kinase, and Rac are directly opposed by
inhibitory signals through SHP-1.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
W. J. Janssen, K. A. McPhillips, M. G. Dickinson, D. J. Linderman, K. Morimoto, Y. Q. Xiao, K. M. Oldham, R. W. Vandivier, P. M. Henson, and S. J. Gardai
Surfactant Proteins A and D Suppress Alveolar Macrophage Phagocytosis via Interaction with SIRP{alpha}
Am. J. Respir. Crit. Care Med.,
July 15, 2008;
178(2):
158 - 167.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Rinna and H. J. Forman
SHP-1 Inhibition by 4-Hydroxynonenal Activates Jun N-Terminal Kinase and Glutamate Cysteine Ligase
Am. J. Respir. Cell Mol. Biol.,
July 1, 2008;
39(1):
97 - 104.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. Tsai and D. E. Discher
Inhibition of "self" engulfment through deactivation of myosin-II at the phagocytic synapse between human cells
J. Cell Biol.,
March 5, 2008;
180(5):
989 - 1003.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. B. Walter, B. W. Raden, R. Zeng, P. Hausermann, I. D. Bernstein, and J. A. Cooper
ITIM-dependent endocytosis of CD33-related Siglecs: role of intracellular domain, tyrosine phosphorylation, and the tyrosine phosphatases, Shp1 and Shp2
J. Leukoc. Biol.,
January 1, 2008;
83(1):
200 - 211.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Canetti, C. H. Serezani, R. G. Atrasz, E. S. White, D. M. Aronoff, and M. Peters-Golden
Activation of Phosphatase and Tensin Homolog on Chromosome 10 Mediates the Inhibition of Fc{gamma}R Phagocytosis by Prostaglandin E2 in Alveolar Macrophages
J. Immunol.,
December 15, 2007;
179(12):
8350 - 8356.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Ai, A. Maturu, W. Johnson, Y. Wang, C. B. Marsh, and S. Tridandapani
The inositol phosphatase SHIP-2 down-regulates Fc{gamma}R-mediated phagocytosis in murine macrophages independently of SHIP-1
Blood,
January 15, 2006;
107(2):
813 - 820.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Munugalavadla, J. Borneo, D. A. Ingram, and R. Kapur
p85{alpha} subunit of class IA PI-3 kinase is crucial for macrophage growth and migration
Blood,
July 1, 2005;
106(1):
103 - 109.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Swanson and A. D. Hoppe
The coordination of signaling during Fc receptor-mediated phagocytosis
J. Leukoc. Biol.,
December 1, 2004;
76(6):
1093 - 1103.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. P. Ganesan, H. Fang, C. B. Marsh, and S. Tridandapani
The Protein-tyrosine Phosphatase SHP-1 Associates with the Phosphorylated Immunoreceptor Tyrosine-based Activation Motif of Fc{gamma}RIIa to Modulate Signaling Events in Myeloid Cells
J. Biol. Chem.,
September 12, 2003;
278(37):
35710 - 35717.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Pengal, L. P. Ganesan, H. Fang, C. B. Marsh, C. L. Anderson, and S. Tridandapani
SHIP-2 Inositol Phosphatase Is Inducibly Expressed in Human Monocytes and Serves to Regulate Fc{gamma} Receptor-mediated Signaling
J. Biol. Chem.,
June 13, 2003;
278(25):
22657 - 22663.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z.-Y. Huang, S. Hunter, M.-K. Kim, Z. K. Indik, and A. D. Schreiber
The effect of phosphatases SHP-1 and SHIP-1 on signaling by the ITIM- and ITAM-containing Fc{gamma} receptors Fc{gamma}RIIB and Fc{gamma}RIIA
J. Leukoc. Biol.,
June 1, 2003;
73(6):
823 - 829.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Zhang, K. Shen, W. Lu, and P. A. Cole
The Role of C-terminal Tyrosine Phosphorylation in the Regulation of SHP-1 Explored via Expressed Protein Ligation
J. Biol. Chem.,
February 7, 2003;
278(7):
4668 - 4674.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
