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Prepublished online as a Blood First Edition Paper on May 24, 2002; DOI 10.1182/blood-2002-04-1017. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-04-1017v1 100/6/2203    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kirveskari, J. Articles by Renkonen, R. Search for Related Content PubMed PubMed Citation Articles by Kirveskari, J. Articles by Renkonen, R. Related Collections Phagocytes Cell Adhesion and Motility Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 September 2002, Vol. 100, No. 6, pp. 2203-2207 PHAGOCYTES Hydrocortisone reduced in vivo, inflammation-induced slow rolling of leukocytes and their extravasation into human conjunctiva Juha Kirveskari, Maaret Helintö, Jukka A. O. Moilanen, Timo Paavonen, Timo M. T. Tervo, and Risto Renkonen From the Rational Drug Design Program, Biomedicum Helsinki; the Department of Bacteriology and Immunology and the Department of Pathology, Haartman Institute, University of Helsinki; Helsinki University Central Hospital (HUCS) Laboratory Diagnostics; and the Department of Ophthalmology, Helsinki University Central Hospital, Helsinki, Finland. Hydrocortisone reduces the number of inflammatory leukocytes within tissues, but thus far the site of action on the multistep adhesion cascade leading to leukocyte extravasation has not been identified. We have recently developed a noninvasive in vivo reflected-light confocal microscopy technique to study this at sites of inflammation in human patients. In the present study, we evaluated the effect of preoperative intravenous hydrocortisone treatment on leukocyte trafficking after conjunctival inflammation induced by cataract surgery in human subjects in vivo. The surgery generated leukocyte rolling along the endothelial lining of conjunctival vessels. While preoperative hydrocortisone did not reduce the number of rolling cells, it significantly raised the velocity of individual rolling leukocytes and concomitantly reduced leukocyte emigration into conjunctival tissue. Immunohistology of conjunctival biopsies excised from the individuals studied provided circumstantial evidence that endothelial P-selectin might play a role in the surgery-induced up-regulation of the leukocyte rolling. Furthermore, hydrocortisone reduced surgery-induced P-selectin induction, suggesting a role for this selectin in the regulation of local leukocyte traffic into sites of inflammation in human conjunctiva. Taken together, these results suggest that control of the rolling velocity might be an effective way to adjust leukocyte traffic in vivo in human subjects. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. K. Toppila-Salmi, J. P. Myller, T. V. M. Torkkeli, J. V. Muhonen, J. A. Renkonen, M. E. Rautiainen, and R. L. O. Renkonen Endothelial L-Selectin Ligands in Sinus Mucosa during Chronic Maxillary Rhinosinusitis Am. J. Respir. Crit. Care Med., June 15, 2005; 171(12): 1350 - 1357. [Abstract] [Full Text] [PDF] M. Helinto, R. Renkonen, T. Tervo, M. Vesaluoma, H. Saaren-Seppala, T. Haahtela, and J. Kirveskari Direct In Vivo Monitoring of Acute Allergic Reactions in Human Conjunctiva J. Immunol., March 1, 2004; 172(5): 3235 - 3242. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020