|
|
Prepublished online as a Blood First Edition Paper on May 31, 2002; DOI 10.1182/blood-2002-01-0047.
 Previous Article | Table of Contents | Next Article 
Blood, 1 October 2002, Vol. 100, No. 7, pp. 2421-2429
HEMATOPOIESIS
Tumor necrosis factor-related apoptosis-inducing ligand induces
monocytic maturation of leukemic and normal myeloid precursors through
a caspase-dependent pathway
Paola Secchiero,
Arianna Gonelli,
Prisco Mirandola,
Elisabetta Melloni,
Loris Zamai,
Claudio Celeghini,
Daniela Milani, and
Giorgio Zauli
From the Department of Morphology and Embryology, Human
Anatomy Section, University of Ferrara; Institute of Morphological
Sciences, University of Urbino; and the Department of Human Normal
Morphology, University of Trieste, Italy.
Treatment of the human HL-60 cell line with tumor necrosis factor
(TNF)-related apoptosis-inducing ligand (TRAIL) resulted in rapid
(6-24 hours) cytotoxicity associated with progressive maturation of the
surviving cells along the monocytic lineage. The occurrence of
monocytic maturation was demonstrated by a significant increase of both
CD14 and CD11b surface expression, the acquisition of morphologic
features typical of mature monocytes, and phagocytic capacity in
TRAIL-treated cultures. By using selective pharmacologic inhibitors, it
was possible to demonstrate that activation of the caspase cascade
played a crucial role in mediating TRAIL cytotoxicity and monocytic
maturation of HL-60 cells. Moreover, experiments performed using
agonistic polyclonal antibodies, which mimic the interactions between
TRAIL and each TRAIL receptor, indicated that TRAIL-R1 was responsible
for mediating the TRAIL-induced maturation. Importantly, the
maturational effects of TRAIL were observed also in primary normal
CD34+ cells, seeded in serum-free liquid cultures for 4 to
8 days in the presence of SCF + GM CSF. After treatment with
TRAIL for 3 additional days, a significant increase in CD14 and CD11b
expression, coupled with an increased number of mature monocytes and
macrophages, was noticed in the absence of cytotoxicity. These data
disclose a novel role for TRAIL as a positive regulator of myeloid
differentiation. Moreover, the dichotomous effect of TRAIL on malignant
cells (early induction of apoptosis and monocytic maturation of the
surviving cells) might have important therapeutic implications for the
treatment of acute myeloid leukemia.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
G. Gobbi, P. Mirandola, C. Carubbi, C. Micheloni, C. Malinverno, P. Lunghi, A. Bonati, and M. Vitale
Phorbol ester-induced PKC{epsilon} down-modulation sensitizes AML cells to TRAIL-induced apoptosis and cell differentiation
Blood,
March 26, 2009;
113(13):
3080 - 3087.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Secchiero, F. Corallini, E. Rimondi, C. Chiaruttini, M. G. di Iasio, A. Rustighi, G. Del Sal, and G. Zauli
Activation of the p53 pathway down-regulates the osteoprotegerin expression and release by vascular endothelial cells
Blood,
February 1, 2008;
111(3):
1287 - 1294.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. Wohlfahrt, B. C. Beard, A. Lieber, and H.-P. Kiem
A Capsid-Modified, Conditionally Replicating Oncolytic Adenovirus Vector Expressing TRAIL Leads to Enhanced Cancer Cell Killing in Human Glioblastoma Models
Cancer Res.,
September 15, 2007;
67(18):
8783 - 8790.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Berent-Maoz, A. M. Piliponsky, I. Daigle, H.-U. Simon, and F. Levi-Schaffer
Human Mast Cells Undergo TRAIL-Induced Apoptosis
J. Immunol.,
February 15, 2006;
176(4):
2272 - 2278.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. S. Teng, M. S. Brandwein-Gensler, M. S. Teixeira, J. A. Martignetti, and D. C. Duffey
A Study of TRAIL Receptors in Squamous Cell Carcinoma of the Head and Neck
Arch Otolaryngol Head Neck Surg,
May 1, 2005;
131(5):
407 - 412.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Morel, R. Audo, M. Hahne, and B. Combe
Tumor Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL) Induces Rheumatoid Arthritis Synovial Fibroblast Proliferation through Mitogen-activated Protein Kinases and Phosphatidylinositol 3-Kinase/Akt
J. Biol. Chem.,
April 22, 2005;
280(16):
15709 - 15718.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Mirandola, C. Ponti, G. Gobbi, I. Sponzilli, M. Vaccarezza, L. Cocco, G. Zauli, P. Secchiero, F. A. Manzoli, and M. Vitale
Activated human NK and CD8+ T cells express both TNF-related apoptosis-inducing ligand (TRAIL) and TRAIL receptors but are resistant to TRAIL-mediated cytotoxicity
Blood,
October 15, 2004;
104(8):
2418 - 2424.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Huppertz and J. C. P. Kingdom
Apoptosis in the Trophoblast--Role of Apoptosis in Placental Morphogenesis
Reproductive Sciences,
September 1, 2004;
11(6):
353 - 362.
[Abstract]
[PDF]
|
|
|
|
|
|
|
P. Secchiero, E. Melloni, M. Heikinheimo, S. Mannisto, R. Di Pietro, A. Iacone, and G. Zauli
TRAIL regulates normal erythroid maturation through an ERK-dependent pathway
Blood,
January 15, 2004;
103(2):
517 - 522.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Secchiero, D. Milani, A. Gonelli, E. Melloni, D. Campioni, D. Gibellini, S. Capitani, and G. Zauli
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) and TNF-{alpha} promote the NF-{kappa}B-dependent maturation of normal and leukemic myeloid cells
J. Leukoc. Biol.,
August 1, 2003;
74(2):
223 - 232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
