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Prepublished online as a Blood First Edition Paper on June 14, 2002; DOI 10.1182/blood-2002-02-0445. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-02-0445v1 100/8/2899    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Howie, D. Articles by Terhorst, C. Search for Related Content PubMed PubMed Citation Articles by Howie, D. Articles by Terhorst, C. Related Collections Signal Transduction Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 October 2002, Vol. 100, No. 8, pp. 2899-2907 IMMUNOBIOLOGY The role of SAP in murine CD150 (SLAM)-mediated T-cell proliferation and interferon  production Duncan Howie, Susumo Okamoto, Svend Rietdijk, Kareem Clarke, Ninghai Wang, Charles Gullo, Joost P. Bruggeman, Stephen Manning, Anthony J. Coyle, Edward Greenfield, Vijay Kuchroo, and Cox Terhorst From the Division of Immunology, Beth Israel Deaconess Medical Center, Harvard Medical School; Department of Adult Oncology, Dana-Farber Cancer Institute and Department of Medicine, Harvard Medical School; Department of Neurology, Center for Neurologic Diseases, Brigham and Women's Hospital and Harvard Medical School, Boston, MA; and Millennium Pharmaceuticals, Cambridge, MA. CD150 (signaling lymphocyte activation molecule [SLAM]) is a self-ligand cell surface glycoprotein expressed on T cells, B cells, macrophages, and dendritic cells. To further explore the role of CD150 signaling in costimulation and TH1 priming we have generated a panel of rat antimouse CD150 monoclonal antibodies. CD150 cell surface expression is up-regulated with rapid kinetics in activated T cells and lipopolysaccharide/interferon  (IFN-)-activated macrophages. Anti-CD150 triggering induces strong costimulation of T cells triggered through CD3. DNA synthesis of murine T cells induced by anti-CD150 is not dependent on SLAM-associated protein (SAP, SH2D1A), because anti-CD150 induces similar levels of DNA synthesis in SAP/ T cells. Antibodies to CD150 also enhance IFN- production both in wild-type and SAP/ T cells during primary stimulation. The level of IFN- production is higher in SAP/ T cells than in wild-type T cells. Anti-CD150 antibodies also synergize with interleukin 12 (IL-12) treatment in up-regulation of IL-12 receptor 2 mRNA during TH1 priming, and inhibit primary TH2 polarization in an IFN--dependent fashion. Cross-linking CD150 on CD4 T cells induces rapid serine phosphorylation of Akt/PKB. We speculate that this is an important pathway contributing to CD150-mediated T-cell proliferation. © 2002 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. A. Jordan, J. M. Fletcher, D. Pellicci, and A. G. Baxter Slamf1, the NKT Cell Control Gene Nkt1 J. Immunol., February 1, 2007; 178(3): 1618 - 1627. [Abstract] [Full Text] [PDF] M. M. McCausland, I. Yusuf, H. Tran, N. Ono, Y. Yanagi, and S. Crotty SAP Regulation of Follicular Helper CD4 T Cell Development and Humoral Immunity Is Independent of SLAM and Fyn Kinase J. Immunol., January 15, 2007; 178(2): 817 - 828. 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