Blood online
Home About Blood Authors Subscriptions Permission Advertising Public Access contact us
 

 
Advanced
Current Issue
First Edition
Future Articles
Archives
Submit to Blood
Search
American Society of Hematology
Meeting Abstracts
Email Alerts
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Right arrow Rights and Permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kyriakides, T. R.
Right arrow Articles by Bornstein, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kyriakides, T. R.
Right arrow Articles by Bornstein, P.
Related Collections
Right arrow Hematopoiesis and Stem Cells
Right arrow Hemostasis, Thrombosis, and Vascular Biology
Right arrow Cell Adhesion and Motility
Social Bookmarking
 Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

arrow to previous article Previous Article  |  Table of Contents  |  Next Article next article arrow

Blood, 15 May 2003, Vol. 101, No. 10, pp. 3915-3923

HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY

Megakaryocytes require thrombospondin-2 for normal platelet formation and function

Themis R. Kyriakides, Ponlapat Rojnuckarin, Michael A. Reidy, Kurt D. Hankenson, Thalia Papayannopoulou, Kenneth Kaushansky, and Paul Bornstein

From the Departments of Biochemistry, Pathology, and Medicine, and the Division of Hematology, University of Washington, Seattle, WA.

Mice that lack the matricellular angiogenesis inhibitor, thrombospondin-2 (TSP2), display a bleeding diathesis, despite normal blood coagulation and the lack of thrombocytopenia. Although platelets do not contain detectable levels of TSP2, TSP2-null platelets are compromised in their ability to aggregate in vivo in response to denudation of the carotid artery endothelium, and in vitro following exposure to adenosine diphosphate (ADP). Megakaryocytes (MKs) show high levels of TSP2 by immunohistochemical analysis of bone marrow. However, when cultured in vitro, MKs contain little TSP2 protein or mRNA. These findings suggest that most TSP2 is acquired from the bone marrow microenvironment. Consistent with this hypothesis, MKs take up recombinant TSP2 in an integrin-dependent manner when it is supplied in the culture medium. Furthermore, uptake of TSP2 in vitro affects MK differentiation and proplatelet formation. The functional significance of this process is supported by the presence of ultrastructural abnormalities in TSP2-null bone marrow, including extensive fragmentation of the peripheral zone in MKs and failure of this zone to form close associations with vascular sinuses. We conclude that the uptake of TSP2 by MKs from the marrow milieu is required for proper MK function and the release of functionally competent platelets.

© 2003 by The American Society of Hematology.
 

Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
BloodHome page
A. Eckly, C. Strassel, M. Freund, J.-P. Cazenave, F. Lanza, C. Gachet, and C. Leon
Abnormal megakaryocyte morphology and proplatelet formation in mice with megakaryocyte-restricted MYH9 inactivation
Blood, April 2, 2009; 113(14): 3182 - 3189.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
J. S. Weinbaum, T. J. Broekelmann, R. A. Pierce, C. C. Werneck, F. Segade, C. S. Craft, R. H. Knutsen, and R. P. Mecham
Deficiency in Microfibril-associated Glycoprotein-1 Leads to Complex Phenotypes in Multiple Organ Systems
J. Biol. Chem., September 12, 2008; 283(37): 25533 - 25543.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Pathol.Home page
M. M. Krady, J. Zeng, J. Yu, S. MacLauchlan, E. A. Skokos, W. Tian, P. Bornstein, W. C. Sessa, and T. R. Kyriakides
Thrombospondin-2 Modulates Extracellular Matrix Remodeling during Physiological Angiogenesis
Am. J. Pathol., September 1, 2008; 173(3): 879 - 891.
[Abstract] [Full Text] [PDF]


Home page
BloodHome page
D. F. Mosher, L. M. Maurer, and C. Britt Carlson
Secreted thrombospondin-1 controls platelet sensitivity to NO
Blood, January 15, 2008; 111(2): 473 - 474.
[Full Text] [PDF]


Home page
Arterioscler. Thromb. Vasc. Bio.Home page
O. I. Stenina, E. J. Topol, and E. F. Plow
Thrombospondins, Their Polymorphisms, and Cardiovascular Disease
Arterioscler Thromb Vasc Biol, September 1, 2007; 27(9): 1886 - 1894.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
Z. M. Ruggeri and G. L. Mendolicchio
Adhesion Mechanisms in Platelet Function
Circ. Res., June 22, 2007; 100(12): 1673 - 1685.
[Abstract] [Full Text] [PDF]


Home page
Circ. Res.Home page
B. Schroen, S. Heymans, U. Sharma, W. M. Blankesteijn, S. Pokharel, J. P.M. Cleutjens, J. G. Porter, C. T.A. Evelo, R. Duisters, R. E.W. van Leeuwen, et al.
Thrombospondin-2 Is Essential for Myocardial Matrix Integrity: Increased Expression Identifies Failure-Prone Cardiac Hypertrophy
Circ. Res., September 3, 2004; 95(5): 515 - 522.
[Abstract] [Full Text] [PDF]



 click for free articles
home about blood authors subscriptions permissions advertising public access contact us
  Copyright © 2003 by American Society of Hematology         Online ISSN: 1528-0020