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Prepublished online as a Blood First Edition Paper on February 13, 2003; DOI 10.1182/blood-2002-06-1902. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-06-1902v1 101/11/4347    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Rivera, A. Articles by Ron, Y. Search for Related Content PubMed PubMed Citation Articles by Rivera, A. Articles by Ron, Y. Related Collections Hematopoiesis Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 June 2003, Vol. 101, No. 11, pp. 4347-4354 HEMATOPOIESIS Host stem cells can selectively reconstitute missing lymphoid lineages in irradiation bone marrow chimeras Amariliz Rivera, Chiann-Chyi Chen, Joseph P. Dougherty, Avraham Ben-Nun, and Yacov Ron From the Department of Molecular Genetics, Microbiology and Immunology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway, NJ; and the Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel. The regulatory elements governing the process of lymphopoiesis from pluripotential stem cells to mature lymphocytes are not well understood. In this study we found that in bone marrow chimeras made by reconstituting lethally irradiated normal mice with bone marrow taken from genetically B-cell–deficient animals (µMT.B6 F1) the B-cell compartment is reconstituted with host-derived B cells. Similarly, in animals reconstituted with bone marrow taken from mice with genetic deficiencies in the development of T cells (TCR–/– F1) or both B and T cells (RAG–/– F1), the missing lymphocyte lineage(s) was specifically reconstituted from host-derived cells. In all chimeras, all other blood lineages were generated from donor-derived stem cells. Control chimeras (B6 F1) had only donor-derived hematopoietic cells as expected. The reconstituted, host-derived lymphoid compartments contained normal functional cell populations as determined by the presence of T cells expressing all 16 common TCR V families, and the presence of all antibody isotypes in the serum. Reconstituted TCR–/– F1 chimeras were also able to mount T-cell proliferative responses to foreign antigens equal to those of control animals. This observation would seem to suggest that during lymphopoietic reconstitution, missing lymphoid lineages can dictate their own reconstitution. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: K.-T. Chang, L. Sefc, O. Psenak, M. Vokurka, and E. Necas Early Fetal Liver Readily Repopulates B Lymphopoiesis in Adult Bone Marrow Stem Cells, February 1, 2005; 23(2): 230 - 239. [Abstract] [Full Text] [PDF]

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