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Related Collections Hemostasis, Thrombosis, and Vascular Biology Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 June 2003, Vol. 101, No. 11, pp. 4479-4484 IMMUNOBIOLOGY Fc receptor transmembrane domains: role in cell surface expression, chain interaction, and phagocytosis Moo-Kyung Kim, Zhen-Yu Huang, Pyoung-Han Hwang, Brian A. Jones, Norihito Sato, Sharon Hunter, Tai-Hee Kim-Han, Randall G. Worth, Zena K. Indik, and Alan D. Schreiber From the Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania School of Medicine, Philadelphia; and the Department of Pediatrics, Chonbuk National University, Medical School, Jeonju, Jeonbuk, Korea. We constructed chimeric receptors to dissect the role of the transmembrane (TM) domain in cell surface expression of and phagocytosis by the chain–dependent Fc receptors FcRIIIA and FcRI. FcR chimeras containing the TM and cytoplasmic (CY) domains of the chain were expressed on the cell surface and mediated an efficient phagocytic signal. In contrast, chimeras containing the FcRIIIA TM were poorly expressed. Receptors containing the FcRI TM and the chain CY but lacking the chain TM also were expressed efficiently and mediated phagocytosis, suggesting that a chain dimer induced by the chain TM is not required for efficient phagocytosis. Cotransfection of FcRI or FcRIIIA with the chimera CD8-- (EC-TM-CY) resulted in FcR cell surface expression and phagocytosis, whereas CD8-CD8-, whose TM does not associate with FcR, allowed cell surface expression of (but not phagocytosis by) FcRI. CD8-CD8- also did not allow surface expression of FcRIIIA. Exchanging FcRI and CD8 TMs indicated that the C-terminal 11 amino acids of the FcRI TM are essential for association of FcRI with the chain and phagocytosis. The data indicate that specific sequences in the FcRIIIA and FcRI TMs govern their different interactions with the chain in cell surface expression and phagocytosis and that chain TM sequences are not required for chain–mediated phagocytosis. The data identify a specific region of the FcRI TM and its asparagine as important for FcRI cell surface expression in the absence of the chain and for distinguishing the FcRI and FcRIIIA phenotypes. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. C. Dale, L. Boxer, and W. C. Liles The phagocytes: neutrophils and monocytes Blood, August 15, 2008; 112(4): 935 - 945. [Abstract] [Full Text] [PDF] J. M. Beekman, C. E. van der Poel, J. A. van der Linden, D. L. C. van den Berg, P. V. E. van den Berghe, J. G. J. van de Winkel, and J. H. W. Leusen Filamin A Stabilizes Fc{gamma}RI Surface Expression and Prevents Its Lysosomal Routing J. 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