|
|
Prepublished online as a Blood First Edition Paper on February 13, 2003; DOI 10.1182/blood-2002-06-1743.
 Previous Article | Table of Contents | Next Article 
Blood, 1 June 2003, Vol. 101, No. 11, pp. 4598-4606
NEOPLASIA
The resistance of B-CLL cells to DNA damage–induced apoptosis defined by DNA microarrays
Laurent Vallat,
Henri Magdelénat,
Hélène Merle-Béral,
Peggy Masdehors,
Gabrielle Potocki de Montalk,
Frédéric Davi,
Mogens Kruhoffer,
Laure Sabatier,
Torben F. Orntoft, and
Jozo Delic
From the Laboratoire de Radiobiologie et Oncologie, Commissariat à l'Energie Atomique, Direction des Sciences du Vivant-Département de Radiopathologie et Radiobiologie (CEA, DSV-DRR), Fontenay aux Roses, France; Laboratoire de Physiopathologie, Institut Curie, Section Médicale, Paris, France; Service d'Hématologie Biologique, Unité Claude Bernard C20, Hôpital Pitié-Salpêtrière, Paris, France; and Molecular Diagnostic Laboratory, Department of Clinical Biochemistry, Aarhus Universitetshospital, Aarhus, Denmark.
B-cell chronic lymphoid leukemia (BCLL) is a highly heterogeneous human malignancy, presumably reflecting specific molecular alterations in gene expression and protein activity that are thought to underlie the variable disease outcome. Most B-CLL cell samples undergo apoptotic death in response to DNA damage. However, a clinically distinct aggressive subset of B-CLL is completely resistant in vitro to irradiation-induced apoptosis. We therefore addressed 2 series of microarray analyses on 4 sensitive and 3 resistant B-CLL cell samples and compared their gene expression patterns before and after apoptotic stimuli. Data analysis pointed out 16 genes whose expression varied at least 2-fold specifically in resistant cells. We validated these selected genes by real-time quantitative reverse transcription–polymerase chain reaction (RT-PCR) on 7 microarray samples and confirmed their altered expression level on 15 additional B-CLL cell samples not included in the microarray analysis. In this manner, in 11 sensitive and 11 resistant B-CLL cell samples tested, 13 genes were found to be specific for all resistant samples: nuclear orphan receptor TR3, major histocompatibility complex (MHC) class II glycoprotein HLA-DQA1, mtmr6, c-myc, c-rel, c-IAP1, mat2A, and fmod were up-regulated, whereas MIP1a/GOS19-1 homolog, stat1, blk, hsp27, and ech1 were down-regulated. In some cases, the expression profile may be dependent on the status of p53. Some of these genes encode general apoptotic factors but also exhibit lymphoid cell specificities that could potentially be linked to the development of lymphoid malignancies (MIP1 , blk, TR3, mtmr6). Taken together, our data define new molecular markers specific to resistant B-CLL subsets that might be of clinical relevance.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. Zou, S.-C. Chang, J. Marjanovic, and P. W. Majerus
MTMR9 Increases MTMR6 Enzyme Activity, Stability, and Role in Apoptosis
J. Biol. Chem.,
January 23, 2009;
284(4):
2064 - 2071.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. B. Kunnumakkara, P. Diagaradjane, S. Guha, A. Deorukhkar, S. Shentu, B. B. Aggarwal, and S. Krishnan
Curcumin Sensitizes Human Colorectal Cancer Xenografts in Nude Mice to {gamma}-Radiation by Targeting Nuclear Factor-{kappa}B-Regulated Gene Products
Clin. Cancer Res.,
April 1, 2008;
14(7):
2128 - 2136.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Gary-Gouy, A. Sainz-Perez, J.-B. Marteau, A. Marfaing-Koka, J. Delic, H. Merle-Beral, P. Galanaud, and A. Dalloul
Natural Phosphorylation of CD5 in Chronic Lymphocytic Leukemia B Cells and Analysis of CD5-Regulated Genes in a B Cell Line Suggest a Role for CD5 in Malignant Phenotype
J. Immunol.,
October 1, 2007;
179(7):
4335 - 4344.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Elmore
Apoptosis: A Review of Programmed Cell Death
Toxicol Pathol,
June 1, 2007;
35(4):
495 - 516.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Yun, L. E. Heisler, I. I. L. Hwang, O. Wilkins, S. K. Lau, M. Hyrcza, B. Jayabalasingham, J. Jin, J. McLaurin, M.-S. Tsao, et al.
Genomic DNA functions as a universal external standard in quantitative real-time PCR
Nucleic Acids Res.,
July 13, 2006;
34(12):
e85 - e85.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. de Totero, R. Meazza, S. Zupo, G. Cutrona, S. Matis, M. Colombo, E. Balleari, I. Pierri, M. Fabbi, M. Capaia, et al.
Interleukin-21 receptor (IL-21R) is up-regulated by CD40 triggering and mediates proapoptotic signals in chronic lymphocytic leukemia B cells
Blood,
May 1, 2006;
107(9):
3708 - 3715.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Secchiero, E. Barbarotto, A. Gonelli, M. Tiribelli, C. Zerbinati, C. Celeghini, C. Agostinelli, S. A. Pileri, and G. Zauli
Potential Pathogenetic Implications of Cyclooxygenase-2 Overexpression in B Chronic Lymphoid Leukemia Cells
Am. J. Pathol.,
December 1, 2005;
167(6):
1599 - 1607.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Deriano, O. Guipaud, H. Merle-Beral, J.-L. Binet, M. Ricoul, G. Potocki-Veronese, V. Favaudon, Z. Maciorowski, C. Muller, B. Salles, et al.
Human chronic lymphocytic leukemia B cells can escape DNA damage-induced apoptosis through the nonhomologous end-joining DNA repair pathway
Blood,
June 15, 2005;
105(12):
4776 - 4783.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Mayr, D. Bund, M. Schlee, A. Moosmann, D. M. Kofler, M. Hallek, and C.-M. Wendtner
Fibromodulin as a novel tumor-associated antigen (TAA) in chronic lymphocytic leukemia (CLL), which allows expansion of specific CD8+ autologous T lymphocytes
Blood,
February 15, 2005;
105(4):
1566 - 1573.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Bertucci, P. Finetti, J. Rougemont, E. Charafe-Jauffret, V. Nasser, B. Loriod, J. Camerlo, R. Tagett, C. Tarpin, G. Houvenaeghel, et al.
Gene Expression Profiling for Molecular Characterization of Inflammatory Breast Cancer and Prediction of Response to Chemotherapy
Cancer Res.,
December 1, 2004;
64(23):
8558 - 8565.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Baran-Marszak, J. Feuillard, I. Najjar, C. Le Clorennec, J.-M. Bechet, I. Dusanter-Fourt, G. W. Bornkamm, M. Raphael, and R. Fagard
Differential roles of STAT1{alpha} and STAT1{beta} in fludarabine-induced cell cycle arrest and apoptosis in human B cells
Blood,
October 15, 2004;
104(8):
2475 - 2483.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
