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Prepublished online as a Blood First Edition Paper on February 27, 2003; DOI 10.1182/blood-2002-05-1504. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-05-1504v1 101/12/4870    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Odeberg, J. Articles by Söderberg-Nauclér, C. Search for Related Content PubMed PubMed Citation Articles by Odeberg, J. Articles by Söderberg-Nauclér, C. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 June 2003, Vol. 101, No. 12, pp. 4870-4877 IMMUNOBIOLOGY Human cytomegalovirus protein pp65 mediates accumulation of HLA-DR in lysosomes and destruction of the HLA-DR -chain Jenny Odeberg, Bodo Plachter, Lars Brandén, and Cecilia Söderberg-Nauclér From the Department of Molecular Medicine, Karolinska Institutet, Stockholm, Sweden; Institute for Virology, University of Mainz, Germany; and Karolinska Institutet, Clinical Research Center (CRC), Huddinge, Sweden. Human cytomegalovirus (HCMV) has developed multiple strategies to escape immune recognition. Here, we demonstrate that HCMV down-regulates HLA-DR expression in infected interferon (IFN-)–stimulated fibroblasts at 1 day after infection. Decreased HLA-DR expression was not observed on cells infected with an HCMV strain lacking the pp65 gene (RVAD65), but was observed on cells transfected with the pp65 gene. HLA-DR expression accumulated in vacuoles near the nucleus in HCMV-infected, but not in uninfected or RVAD65-infected cells. In addition, the HLA-DR -chain, but not the -chain or HLA-DM, was degraded in HCMV-infected but not in RVAD65-infected cells. Thus, the HCMV protein pp65 mediates decreased expression of HLA-DR, by mediating an accumulation of HLA class II molecules in lysosomes that results in degradation of the HLA-DR -chain. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Chevillotte, S. Landwehr, L. Linta, G. Frascaroli, A. Luske, C. Buser, T. 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