|
|
Prepublished online as a Blood First Edition Paper on March 6, 2003; DOI 10.1182/blood-2002-10-3159.
 Previous Article | Table of Contents | Next Article 
Blood, 15 June 2003, Vol. 101, No. 12, pp. 4916-4922
IMMUNOBIOLOGY
LFA-1 is required for retention of effector CD8 T cells in mouse lungs
Jayant Thatte,
Vrushali Dabak,
Mark B. Williams,
Thomas J. Braciale, and
Klaus Ley
From the Department of Biomedical Engineering, Beirne B. Carter Center for Immunology Research, the Department of Radiology, and the Cardiovascular Research Center, University of Virginia Health Sciences Center, Charlottesville, VA.
The adhesion molecules involved in the migration and retention of activated effector CD8 T cells in the lung microcirculation and their recruitment into lung tissue are largely unknown. Here, we have analyzed the role of lymphocyte function–associated antigen-1 (LFA-1) and very late antigen-4 (VLA-4) on adhesion of influenza hemagglutinin (HA)–specific CD8 T-cell clone D4 under shear conditions in an in vitro binding assay and in an in vivo homing assay to the lungs of naive or transgenic Balb/c mice expressing HA (HA-Tg) by a lung-specific promoter. Blocking LFA-1 or intercellular adhesion molecule 1 (ICAM-1) significantly inhibited adhesion of D4 cells to lung vascular endothelium and parenchyma of lung sections. However, blocking VLA-4 or vascular cell adhesion molecule 1 (VCAM-1) had no effect on cell adhesion. Blocking LFA-1 in vivo significantly delayed lethal injury following adoptive transfer of D4 cells into HA-Tg mice as assessed by weight loss and histology. Residence time of adoptively transferred Indium 111 (111In)–labeled D4 cells in lungs of normal and HA-Tg mice as analyzed by dual modality imaging revealed a significantly shorter transit time of 4 hours for the D4 cells upon in vivo blockade of LFA-1. These results demonstrate a crucial role for LFA-1 in retention of activated CD8 T cells in normal mouse lungs and in the progression of lethal injury in HA-Tg mice.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
N. K. Polakos, I. Klein, M. V. Richter, D. M. Zaiss, M. Giannandrea, I. N. Crispe, and D. J. Topham
Early Intrahepatic Accumulation of CD8+ T Cells Provides a Source of Effectors for Nonhepatic Immune Responses
J. Immunol.,
July 1, 2007;
179(1):
201 - 210.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Zhang, E. P. Wojcikiewicz, and V. T. Moy
Dynamic Adhesion of T Lymphocytes to Endothelial Cells Revealed by Atomic Force Microscopy
Experimental Biology and Medicine,
September 1, 2006;
231(8):
1306 - 1312.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Basit, J. Reutershan, M. A. Morris, M. Solga, C. E. Rose Jr., and K. Ley
ICAM-1 and LFA-1 play critical roles in LPS-induced neutrophil recruitment into the alveolar space
Am J Physiol Lung Cell Mol Physiol,
August 1, 2006;
291(2):
L200 - L207.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ghosh, A. A. Chackerian, C. M. Parker, C. M. Ballantyne, and S. M. Behar
The LFA-1 adhesion molecule is required for protective immunity during pulmonary Mycobacterium tuberculosis infection.
J. Immunol.,
April 15, 2006;
176(8):
4914 - 4922.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. K. Whitmire, N. Benning, and J. L. Whitton
Precursor Frequency, Nonlinear Proliferation, and Functional Maturation of Virus-Specific CD4+ T Cells.
J. Immunol.,
March 1, 2006;
176(5):
3028 - 3036.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Schreiber, S. Ehlers, S. Aly, A. Holscher, S. Hartmann, M. Lipp, J. B. Lowe, and C. Holscher
Selectin Ligand-Independent Priming and Maintenance of T Cell Immunity during Airborne Tuberculosis
J. Immunol.,
January 15, 2006;
176(2):
1131 - 1140.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. H. Ely, T. Cookenham, A. D. Roberts, and D. L. Woodland
Memory T Cell Populations in the Lung Airways Are Maintained by Continual Recruitment
J. Immunol.,
January 1, 2006;
176(1):
537 - 543.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. A. W. Claassen, P. A. A. van der Kant, Z. S. Rychnavska, G. M. van Bleek, A. J. Easton, and R. G. van der Most
Activation and Inactivation of Antiviral CD8 T Cell Responses during Murine Pneumovirus Infection
J. Immunol.,
November 15, 2005;
175(10):
6597 - 6604.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. L. Woodland and I. Scott
T Cell Memory in the Lung Airways
Proceedings of the ATS,
August 1, 2005;
2(2):
126 - 131.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. W. Cruise, H. M. Melief, J. Lukens, C. Soguero, and Y. S. Hahn
Increased Fas ligand expression of CD4+ T cells by HCV core induces T cell-dependent hepatic inflammation
J. Leukoc. Biol.,
August 1, 2005;
78(2):
412 - 425.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kandula and C. Abraham
LFA-1 on CD4+ T Cells Is Required for Optimal Antigen-Dependent Activation In Vivo
J. Immunol.,
October 1, 2004;
173(7):
4443 - 4451.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. C. Whipple, R. S. Shanahan, A. H. Ditto, R. P. Taylor, and M. A. Lindorfer
Analyses of the In Vivo Trafficking of Stoichiometric Doses of an Anti-Complement Receptor 1/2 Monoclonal Antibody Infused Intravenously in Mice
J. Immunol.,
August 15, 2004;
173(4):
2297 - 2306.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. W. Lawrence and T. J. Braciale
Activation, Differentiation, and Migration of Naive Virus-Specific CD8+ T Cells during Pulmonary Influenza Virus Infection
J. Immunol.,
July 15, 2004;
173(2):
1209 - 1218.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. John and I. N. Crispe
Passive and Active Mechanisms Trap Activated CD8+ T Cells in the Liver
J. Immunol.,
May 1, 2004;
172(9):
5222 - 5229.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. H. Ely, A. D. Roberts, and D. L. Woodland
Cutting Edge: Effector Memory CD8+ T Cells in the Lung Airways Retain the Potential to Mediate Recall Responses
J. Immunol.,
October 1, 2003;
171(7):
3338 - 3342.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
