|
|
Prepublished online as a Blood First Edition Paper on September 5, 2002; DOI 10.1182/blood-2002-05-1553.
 Previous Article | Table of Contents | Next Article 
Blood, 15 January 2003, Vol. 101, No. 2, pp. 541-544
HEMATOPOIESIS
Megakaryocyte polyploidization is associated with a functional
gene amplification
Hana Raslova,
Lydia Roy,
Claire Vourc'h,
Jean Pierre Le
Couedic,
Olivier Brison,
Didier Metivier,
Jean Feunteun,
Guido Kroemer,
Najet Debili, and
William Vainchenker
From INSERM U362, and Centre National de la
Recherche Scientifique (CNRS) UMR 1599, Institut Gustave Roussy,
Villejuif, France; and INSERM U309, Institut Albert Bonniot, La
Tronche, France.
It is believed that polyploidy induces an orchestrated increase in
gene expression. To know whether all alleles remain functional during
megakaryocyte polyploidization, we used a well-established fluorescence in situ hybridization technique which allows one to
simultaneously detect pre-mRNAs and assess ploidy level in a single
cell. All alleles of GPIIb, GPIIIa,
VWF,  -actin, hsp70, c-mpl, Fli-1, and FOG-1 genes are
transcriptionally active in megakaryocytes from 4N to 32N. All X
chromosomes in male cells are transcriptionally active but only half of
them are transcriptionally active in female megakaryocytes, as
revealed by the transcriptional activity of the GATA-1
gene. Nuclear untranslated XIST RNA accumulates on the inactivated X
chromosomes, indicating that they are subjected to a normal
inactivation process. Altogether, our results demonstrate that
megakaryocyte polyploidization results in a functional gene amplification whose likely function is an increase in protein synthesis
parallel with cell enlargement.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
C. Lavenu-Bombled, B. Izac, F. Legrand, M. Cambot, A. Vigier, J.-M. Masse, and A. Dubart-Kupperschmitt
Glycoprotein Ib{alpha} Promoter Drives Megakaryocytic Lineage-Restricted Expression After Hematopoietic Stem Cell Transduction Using a Self-Inactivating Lentiviral Vector
Stem Cells,
June 1, 2007;
25(6):
1571 - 1577.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Raslova, A. Kauffmann, D. Sekkai, H. Ripoche, F. Larbret, T. Robert, D. T. Le Roux, G. Kroemer, N. Debili, P. Dessen, et al.
Interrelation between polyploidization and megakaryocyte differentiation: a gene profiling approach
Blood,
April 15, 2007;
109(8):
3225 - 3234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. N. Carter, G. Tolhurst, G. Walmsley, M. Vizuete-Forster, N. Miller, and M. P. Mahaut-Smith
Molecular and electrophysiological characterization of transient receptor potential ion channels in the primary murine megakaryocyte
J. Physiol.,
October 1, 2006;
576(1):
151 - 162.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
