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Prepublished online as a Blood First Edition Paper on August 1, 2002; DOI 10.1182/blood-2002-02-0500. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-02-0500v1 101/2/747    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Willekens, F. L. A. Articles by Werre, J. M. Search for Related Content PubMed PubMed Citation Articles by Willekens, F. L. A. Articles by Werre, J. M. Related Collections Red Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 January 2003, Vol. 101, No. 2, pp. 747-751 RED CELLS Hemoglobin loss from erythrocytes in vivo results from spleen-facilitated vesiculation Frans L. A. Willekens, Bregt Roerdinkholder-Stoelwinder, Yvonne A. M. Groenen-Döpp, Harry J. Bos, Giel J. C. G. M. Bosman, Annegeet G. van den Bos, Arie J. Verkleij, and Jan M. Werre From the Department of Clinical Chemistry, Rijnstate Hospital, Arnhem; Sanquin Blood Bank Zuid Oost Nederland; the Department for Bloodtransfusion and Transplantation Immunology and the Department of Biochemistry, University Medical Center, Nijmegen; and the Department of Molecular Cellbiology, University of Utrecht, The Netherlands. Previous studies have shown that approximately 20% of hemoglobin is lost from circulating red blood cells (RBCs), mainly during the second half of the cells' life span. Because hemoglobin-containing vesicles are known to circulate in plasma, these vesicles were isolated. Flow cytometry studies showed that most RBC-derived vesicles contain hemoglobin with all hemoglobin components present. The hemoglobin composition of the vesicles resembled that of old RBCs. RBC cohort studies using isotope-labeled glycine have been described, which showed a continuous presence of this label in hemoglobin degradation products. The label concentration of these products increased during the second half of the RBC life span, accompanied by a decrease within the RBC. It is concluded that the hemoglobin loss from circulating RBCs of all ages can be explained by shedding hemoglobin-containing vesicles. This loss occurs predominantly in older RBCs. Apparently the spleen facilitates this process since asplenia vesicle retention within RBCs of all ages has been described, accompanied by an increase in the percentage of total HbA1. The present study shows that in old RBCs of asplenic individuals, the decrease of hemoglobin content per cell such as seen in old RBCs of control individuals is absent due to an increase in the absolute amount of HbA1c and HbA1e2. It is concluded that hemoglobin-containing vesicles within old RBCs are "pitted" by the spleen. © 2003 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. L. A. Willekens, J. M. Werre, J. K. Kruijt, B. Roerdinkholder-Stoelwinder, Y. A. M. Groenen-Dopp, A. G. van den Bos, G. J. C. G. M. Bosman, and T. J. C. van Berkel Liver Kupffer cells rapidly remove red blood cell-derived vesicles from the circulation by scavenger receptors Blood, March 1, 2005; 105(5): 2141 - 2145. [Abstract] [Full Text] [PDF] E. M. Sloand, L. Mainwaring, K. Keyvanfar, J. Chen, J. Maciejewski, H. G. Klein, and N. S. Young Transfer of glycosylphosphatidylinositol-anchored proteins to deficient cells after erythrocyte transfusion in paroxysmal nocturnal hemoglobinuria Blood, December 1, 2004; 104(12): 3782 - 3788. [Abstract] [Full Text] [PDF]

	Copyright © 2003 by American Society of Hematology         Online ISSN: 1528-0020