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Prepublished online as a Blood First Edition Paper on September 19, 2002; DOI 10.1182/blood-2002-06-1828. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-06-1828v1 101/3/1030    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wang, Y.-H. Articles by Cooper, M. D. Search for Related Content PubMed PubMed Citation Articles by Wang, Y.-H. Articles by Cooper, M. D. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 February 2003, Vol. 101, No. 3, pp. 1030-1037 IMMUNOBIOLOGY V(D)J recombinatorial repertoire diversification during intraclonal pro-B to B-cell differentiation Yui-Hsi Wang, Zhixin Zhang, Peter D. Burrows, Hiromi Kubagawa, S. Louis Bridges Jr, Harry W. Findley, and Max D. Cooper From the Division of Developmental and Clinical Immunology, and Departments of Medicine, Pediatrics, Pathology, and Microbiology, University of Alabama at Birmingham and the Howard Hughes Medical Institute, Birmingham, AL; and Division of Pediatric Hematology/Oncology/Bone Marrow Transplantation, Emory University School of Medicine, Atlanta, GA. The initial B-cell repertoire is generated by combinatorial immunoglobulin V(D)J gene segment rearrangements that occur in a preferential sequence. Because cellular proliferation occurs during the course of these rearrangement events, it has been proposed that intraclonal diversification occurs during this phase of B-cell development. An opportunity to examine this hypothesis directly was provided by the identification of a human acute lymphoblastic leukemic cell line that undergoes spontaneous differentiation from pro-B cell to the pre-B and B-cell stages with concomitant changes in the gene expression profile that normally occur during B-cell differentiation. After confirming the clonality of the progressively differentiating cells, an analysis of immunoglobulin genes and transcripts indicated that pro-B cell members marked by the same DJ rearrangement generated daughter B cells with multiple VH and VL gene segment rearrangements. These findings validate the principle of intraclonal V(D)J diversification during B-cell generation and define a manipulable model of human B-cell differentiation. © 2003 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. M. Collins, M. Ikutani, D. Puiu, G. A. Buck, A. Nadkarni, and B. Gaeta Partitioning of Rearranged Ig Genes by Mutation Analysis Demonstrates D-D Fusion and V Gene Replacement in the Expressed Human Repertoire J. Immunol., January 1, 2004; 172(1): 340 - 348. [Abstract] [Full Text] [PDF]

	Copyright © 2003 by American Society of Hematology         Online ISSN: 1528-0020