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Prepublished online as a Blood First Edition Paper on October 3, 2002; DOI 10.1182/blood-2002-05-1435.
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Blood, 1 February 2003, Vol. 101, No. 3, pp. 1181-1184
PHAGOCYTES
Brief report
Chemoattractant receptor-stimulated F-actin polymerization in
the human neutrophil is signaled by 2 distinct pathways
David Chodniewicz and
Doncho V. Zhelev
From the Department of Mechanical Engineering and
Materials Science, Duke University, Durham, NC.
We characterized the overall rate of F-actin polymerization
in the pseudopod region by measuring the rate of extension of single
pseudopods stimulated by f-Met-Leu-Phe. The rate of pseudopod extension
was measured in the presence of inhibitors for signaling molecules that
are known to be involved in motility. Our data show the existence of 2 distinct signaling pathways of actin polymerization in the
pseudopod region: a phosphoinositide 3-kinase  (PI3K)-dependent and -independent pathway. The PI3K dependent
signaling of F-actin polymerization also depends on protein kinase C
 and protein kinase B (Akt/PKB). The PI3K-independent pathway
depends on GTPase RhoA, the RhoA ROCK kinase, Src
family tyrosine kinases, and NADPH, and is modulated by cAMP.
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