|
|
Prepublished online as a Blood First Edition Paper on September 5, 2002; DOI 10.1182/blood-2002-05-1327.
 Previous Article | Table of Contents | Next Article 
Blood, 1 February 2003, Vol. 101, No. 3, pp. 961-969
IMMUNOBIOLOGY
ICSBP/IRF-8 retrovirus transduction rescues dendritic cell
development in vitro
Hideki Tsujimura,
Tomohiko Tamura,
Celine Gongora,
Julio Aliberti,
Caetano Reis e Sousa,
Alan Sher, and
Keiko Ozato
From the Laboratory of Molecular Growth Regulation,
National Institute of Child Health and Human Development, and
Laboratory of Parasite Diseases, National Institute of Allergy and
Infectious Diseases, National Institutes of Health (NIH), Bethesda, MD;
and Immunobiology Laboratory, Cancer Research United Kingdom, London
Research Institute, London, United Kingdom.
Dendritic cells (DCs) develop from bone marrow (BM)
progenitor cells and mature in response to external signals to elicit functions important for innate and adaptive immunity. Interferon consensus sequence binding protein (ICSBP; also called interferon regulatory factor 8 [IRF-8]) is a hematopoietic
cell-specific transcription factor expressed in BM progenitor cells
that contributes to myeloid cell development. In light of our earlier
observation that ICSBP / mice lack CD8 +
DCs, we investigated the role of ICSBP in DC development in vitro in
the presence of Flt3 ligand. Immature ICSBP/ DCs
developed from BM progenitor cells showed assorted defects, did not
mature in response to activation signals, and failed to express
CD8 and interleukin 12 (IL-12) p40, a feature consistent with
ICSBP/ DCs in vivo. We show that retroviral
introduction of ICSBP restores the development of immature DCs that can
fully mature on activation signals. All the defects seen with
ICSBP/ DCs were corrected after ICSBP transduction,
including the expression of CD8 and IL-12 p40 as well as major
histocompatability complex class II and other costimulatory molecules.
ICSBP is known to regulate gene expression by interacting with partner
proteins PU.1 and IRFs, thereby binding to target elements ISRE and
EICE. Analysis of a series of ICSBP mutants showed that the
intact DNA-binding activity as well as the ability to interact with
partner proteins are required for the restoration of DC
development/maturation, pointing to the transcriptional function of
ICSBP as a basis of restoration. Taken together, this study identifies
ICSBP as a factor critical for both early differentiation and final
maturation of DCs.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
P. Tailor, T. Tamura, H. C. Morse III, and K. Ozato
The BXH2 mutation in IRF8 differentially impairs dendritic cell subset development in the mouse
Blood,
February 15, 2008;
111(4):
1942 - 1945.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Turcotte, S. Gauthier, D. Malo, M. Tam, M. M. Stevenson, and P. Gros
Icsbp1/IRF-8 Is Required for Innate and Adaptive Immune Responses against Intracellular Pathogens
J. Immunol.,
August 15, 2007;
179(4):
2467 - 2476.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. J. Kong, D. E. Anderson, C. H. Lee, M. K. Jang, T. Tamura, P. Tailor, H. K. Cho, J. Cheong, H. Xiong, H. C. Morse III, et al.
Cutting Edge: Autoantigen Ro52 Is an Interferon Inducible E3 Ligase That Ubiquitinates IRF-8 and Enhances Cytokine Expression in Macrophages
J. Immunol.,
July 1, 2007;
179(1):
26 - 30.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Nakano, C. Nishiyama, S. Kanada, Y. Niwa, N. Shimokawa, H. Ushio, M. Nishiyama, K. Okumura, and H. Ogawa
Involvement of mast cells in IL-12/23 p40 production is essential for survival from polymicrobial infections
Blood,
June 1, 2007;
109(11):
4846 - 4855.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Yadav and N. Sarvetnick
B7-2 Regulates Survival, Phenotype, and Function of APCs
J. Immunol.,
May 15, 2007;
178(10):
6236 - 6241.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Dror, N. Rave-Harel, A. Burchert, A. Azriel, T. Tamura, P. Tailor, A. Neubauer, K. Ozato, and B.-Z. Levi
Interferon Regulatory Factor-8 Is Indispensable for the Expression of Promyelocytic Leukemia and the Formation of Nuclear Bodies in Myeloid Cells
J. Biol. Chem.,
February 23, 2007;
282(8):
5633 - 5640.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Mattei, G. Schiavoni, P. Borghi, M. Venditti, I. Canini, P. Sestili, I. Pietraforte, H. C. Morse III, C. Ramoni, F. Belardelli, et al.
ICSBP/IRF-8 differentially regulates antigen uptake during dendritic-cell development and affects antigen presentation to CD4+ T cells
Blood,
July 15, 2006;
108(2):
609 - 617.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. H. Lee, M. Melchers, H. Wang, T. A. Torrey, R. Slota, C.-F. Qi, J. Y. Kim, P. Lugar, H. J. Kong, L. Farrington, et al.
Regulation of the germinal center gene program by interferon (IFN) regulatory factor 8/IFN consensus sequence-binding protein
J. Exp. Med.,
January 23, 2006;
203(1):
63 - 72.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Laricchia-Robbio, T. Tamura, T. Karpova, B. L. Sprague, J. G. McNally, and K. Ozato
Partner-regulated interaction of IFN regulatory factor 8 with chromatin visualized in live macrophages
PNAS,
October 4, 2005;
102(40):
14368 - 14373.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Dominguez-Soto, A. Puig-Kroger, M. A. Vega, and A. L. Corbi
PU.1 Regulates the Tissue-specific Expression of Dendritic Cell-specific Intercellular Adhesion Molecule (ICAM)-3-grabbing Nonintegrin
J. Biol. Chem.,
September 30, 2005;
280(39):
33123 - 33131.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Takahashi, L. Li, M. Kamiryo, T. Asteriou, A. Moustakas, H. Yamashita, and P. Heldin
Hyaluronan Fragments Induce Endothelial Cell Differentiation in a CD44- and CXCL1/GRO1-dependent Manner
J. Biol. Chem.,
June 24, 2005;
280(25):
24195 - 24204.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Prakash, E. Smith, C.-k. Lee, and D. E. Levy
Tissue-specific Positive Feedback Requirements for Production of Type I Interferon following Virus Infection
J. Biol. Chem.,
May 13, 2005;
280(19):
18651 - 18657.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Turcotte, S. Gauthier, A. Tuite, A. Mullick, D. Malo, and P. Gros
A mutation in the Icsbp1 gene causes susceptibility to infection and a chronic myeloid leukemia-like syndrome in BXH-2 mice
J. Exp. Med.,
March 21, 2005;
201(6):
881 - 890.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Tamura, P. Tailor, K. Yamaoka, H. J. Kong, H. Tsujimura, J. J. O'Shea, H. Singh, and K. Ozato
IFN Regulatory Factor-4 and -8 Govern Dendritic Cell Subset Development and Their Functional Diversity
J. Immunol.,
March 1, 2005;
174(5):
2573 - 2581.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. McKenna, A.-S. Beignon, and N. Bhardwaj
Plasmacytoid Dendritic Cells: Linking Innate and Adaptive Immunity
J. Virol.,
January 1, 2005;
79(1):
17 - 27.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Suzuki, K. Honma, T. Matsuyama, K. Suzuki, K. Toriyama, I. Akitoyo, K. Yamamoto, T. Suematsu, M. Nakamura, K. Yui, et al.
From the Cover: Critical roles of interferon regulatory factor 4 in CD11bhighCD8{alpha}- dendritic cell development
PNAS,
June 15, 2004;
101(24):
8981 - 8986.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Tsujimura, T. Tamura, H. J. Kong, A. Nishiyama, K. J. Ishii, D. M. Klinman, and K. Ozato
Toll-Like Receptor 9 Signaling Activates NF-{kappa}B through IFN Regulatory Factor-8/IFN Consensus Sequence Binding Protein in Dendritic Cells
J. Immunol.,
June 1, 2004;
172(11):
6820 - 6827.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Schmidt, J. Bies, T. Tamura, K. Ozato, and L. Wolff
The interferon regulatory factor ICSBP/IRF-8 in combination with PU.1 up-regulates expression of tumor suppressor p15Ink4b in murine myeloid cells
Blood,
June 1, 2004;
103(11):
4142 - 4149.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Schiavoni, F. Mattei, P. Borghi, P. Sestili, M. Venditti, H. C. Morse III, F. Belardelli, and L. Gabriele
ICSBP is critically involved in the normal development and trafficking of Langerhans cells and dermal dendritic cells
Blood,
March 15, 2004;
103(6):
2221 - 2228.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Tamura, H. J. Kong, C. Tunyaplin, H. Tsujimura, K. Calame, and K. Ozato
ICSBP/IRF-8 inhibits mitogenic activity of p210 Bcr/Abl in differentiating myeloid progenitor cells
Blood,
December 15, 2003;
102(13):
4547 - 4554.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Tsujimura, T. Tamura, and K. Ozato*
Cutting Edge: IFN Consensus Sequence Binding Protein/IFN Regulatory Factor 8 Drives the Development of Type I IFN-Producing Plasmacytoid Dendritic Cells
J. Immunol.,
February 1, 2003;
170(3):
1131 - 1135.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
