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Prepublished online as a Blood First Edition Paper on October 31, 2002; DOI 10.1182/blood-2002-03-0831. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-03-0831v1 101/6/2300    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yoshida, S. Articles by Ishii, A. Search for Related Content PubMed PubMed Citation Articles by Yoshida, S. Articles by Ishii, A. Related Collections Immunobiology Red Cells Immunotherapy Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 March 2003, Vol. 101, No. 6, pp. 2300-2306 IMMUNOBIOLOGY T-cell activation and cytokine production via a bispecific single-chain antibody fragment targeted to blood-stage malaria parasites Shigeto Yoshida, Tominari Kobayashi, Hiroyuki Matsuoka, Chisato Seki, William L. Gosnell, Sandra P. Chang, and Akira Ishii From the Department of Medical Zoology, Jichi Medical School, Tochigi, Japan; and the Department of Tropical Medicine and Medical Microbiology, John A. Burns School of Medicine, Leahi Hospital, University of Hawaii at Manoa, Honolulu, HI. A novel bispecific single-chain antibody fragment (biscFv) has been constructed to address the possibility of a new approach to malaria therapeutic drug development. The biscFv consists of 2 different single-chain antibody fragments linked by a flexible peptide linker (Gly4-Ser)3. Of the 2 scFv fragments, one is directed against a conserved epitope of the 19-kDa C-terminal fragment of the major surface protein of human malignant malaria parasite, Plasmodium falciparum, and the other is directed against the CD3 antigen of human T cells. The biscFv expressed by a recombinant baculovirus retained the antigen-binding properties of the corresponding univalent single-chain antibody fragments and formed a bridge between P falciparum and T cells. In cooperation with T cells, the biscFv specifically induced not only interferon  and tumor necrosis factor , but also a significant increase of merozoite phagocytosis and growth inhibition of P falciparum in vitro. Thus, the biscFv possesses highly selective malaria-targeting properties and stimulates T cells to induce cytokines, presumably resulting in activation of macrophages, neutrophils, and natural killer cells, and parasite killing in vivo. © 2003 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Yoshida, T. Sudo, M. Niimi, L. Tao, B. Sun, J. Kambayashi, H. Watanabe, E. Luo, and H. Matsuoka Inhibition of collagen-induced platelet aggregation by anopheline antiplatelet protein, a saliva protein from a malaria vector mosquito Blood, February 15, 2008; 111(4): 2007 - 2014. [Abstract] [Full Text] [PDF]

	Copyright © 2003 by American Society of Hematology         Online ISSN: 1528-0020