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Prepublished online as a Blood First Edition Paper on October 24, 2002; DOI 10.1182/blood-2002-03-0973.
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Blood, 15 March 2003, Vol. 101, No. 6, pp. 2314-2317
IMMUNOBIOLOGY
Brief report
Granulocyte-colony stimulating factor increases
CD123hi blood dendritic cells with altered CD62L and
CCR7 expression
Slavica Vuckovic,
Min Kim,
Dailal Khalil,
Cameron J. Turtle,
Georgina V. Crosbie,
Noel Williams,
Len Brown,
Katrina Williams,
Cathryn Kelly,
Penny Stravos,
Robyn Rodwell,
Geoff R. Hill,
Susan Wright,
Kerry Taylor,
Devinder Gill,
Paula Marlton,
Ken Bradstock, and
Derek N. J. Hart
From the Mater Medical Research Institute, South
Brisbane, Queensland, Australia; the Mater Misericordiae Hospital,
South Brisbane, Queensland, Australia; the Princess Alexandra Hospital,
Queensland, Australia; and the Blood & Bone Marrow Transplant Service,
Westmead Hospital, Westmead, Australia.
Changes in blood dendritic cell (BDC) counts
(CD123hiBDC and CD11c+BDC) and expression of
CD62L, CCR7, and CD49d were analyzed in healthy donors, multiple
myeloma (MM), and non-Hodgkin lymphoma (NHL) patients, who received
granulocyte-colony stimulating factor (G-CSF) containing peripheral
blood stem cell (PBSC) mobilization protocols. Low-dose G-CSF in
healthy donors (8-10 µg/kg/d subcutaneously) and high-dose G-CSF in
patients (30 µg/kg/d) increased CD123hiBDC (2- to
22-fold, mean 3.7 × 106/L-17.7 × 106/L
and 1.9 × 106/L-12.0 × 106/L) in healthy
donors and MM but decreased CD11c+BDC (2- to 10-fold, mean
5.7 × 106/L-1.6 × 106/L) in NHL patients,
on the day of apheresis, compared with steady state. After apheresis,
CD123hiBDC counts remained high, whereas low
CD11c+BDC counts tended to recover in the following 2-5 days. Down-regulation of CD62L and up-regulation of CCR7 on
CD123hiBDC were found in most healthy donors and MM
patients. CD49d expression was unchanged. Thus, PBSC mobilization may
change BDC counts by altering molecules necessary for BDC homing from
blood into tissues.
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