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Prepublished online as a Blood First Edition Paper on October 24, 2002; DOI 10.1182/blood-2002-03-0973. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-03-0973v1 101/6/2314    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Vuckovic, S. Articles by Hart, D. N. J. Search for Related Content PubMed PubMed Citation Articles by Vuckovic, S. Articles by Hart, D. N. J. Related Collections Immunobiology Neoplasia Brief Reports Clinical Trials and Observations Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 March 2003, Vol. 101, No. 6, pp. 2314-2317 IMMUNOBIOLOGY Brief report Granulocyte-colony stimulating factor increases CD123hi blood dendritic cells with altered CD62L and CCR7 expression Slavica Vuckovic, Min Kim, Dailal Khalil, Cameron J. Turtle, Georgina V. Crosbie, Noel Williams, Len Brown, Katrina Williams, Cathryn Kelly, Penny Stravos, Robyn Rodwell, Geoff R. Hill, Susan Wright, Kerry Taylor, Devinder Gill, Paula Marlton, Ken Bradstock, and Derek N. J. Hart From the Mater Medical Research Institute, South Brisbane, Queensland, Australia; the Mater Misericordiae Hospital, South Brisbane, Queensland, Australia; the Princess Alexandra Hospital, Queensland, Australia; and the Blood & Bone Marrow Transplant Service, Westmead Hospital, Westmead, Australia. Changes in blood dendritic cell (BDC) counts (CD123hiBDC and CD11c+BDC) and expression of CD62L, CCR7, and CD49d were analyzed in healthy donors, multiple myeloma (MM), and non-Hodgkin lymphoma (NHL) patients, who received granulocyte-colony stimulating factor (G-CSF) containing peripheral blood stem cell (PBSC) mobilization protocols. Low-dose G-CSF in healthy donors (8-10 µg/kg/d subcutaneously) and high-dose G-CSF in patients (30 µg/kg/d) increased CD123hiBDC (2- to 22-fold, mean 3.7 × 106/L-17.7 × 106/L and 1.9 × 106/L-12.0 × 106/L) in healthy donors and MM but decreased CD11c+BDC (2- to 10-fold, mean 5.7 × 106/L-1.6 × 106/L) in NHL patients, on the day of apheresis, compared with steady state. After apheresis, CD123hiBDC counts remained high, whereas low CD11c+BDC counts tended to recover in the following 2-5 days. Down-regulation of CD62L and up-regulation of CCR7 on CD123hiBDC were found in most healthy donors and MM patients. CD49d expression was unchanged. Thus, PBSC mobilization may change BDC counts by altering molecules necessary for BDC homing from blood into tissues. © 2003 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. S. Morris, K. P. A. MacDonald, and G. R. Hill Stem cell mobilization with G-CSF analogs: a rational approach to separate GVHD and GVL? Blood, May 1, 2006; 107(9): 3430 - 3435. [Abstract] [Full Text] [PDF] S. Rutella, F. Zavala, S. Danese, H. Kared, and G. Leone Granulocyte Colony-Stimulating Factor: A Novel Mediator of T Cell Tolerance J. Immunol., December 1, 2005; 175(11): 7085 - 7091. [Abstract] [Full Text] [PDF] F. F. Fagnoni, B. Oliviero, G. Giorgiani, P. De Stefano, A. Deho, C. Zibera, N. Gibelli, R. Maccario, G. Da Prada, M. Zecca, et al. Reconstitution dynamics of plasmacytoid and myeloid dendritic cell precursors after allogeneic myeloablative hematopoietic stem cell transplantation Blood, July 1, 2004; 104(1): 281 - 289. [Abstract] [Full Text] [PDF]

	Copyright © 2003 by American Society of Hematology         Online ISSN: 1528-0020