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Prepublished online as a Blood First Edition Paper on November 27, 2002; DOI 10.1182/blood-2002-07-2265. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-07-2265v1 101/7/2601    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ito, A. Articles by Kitamura, Y. Search for Related Content PubMed PubMed Citation Articles by Ito, A. Articles by Kitamura, Y. Related Collections Hematopoiesis and Stem Cells Cell Adhesion and Motility Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 April 2003, Vol. 101, No. 7, pp. 2601-2608 HEMATOPOIESIS SgIGSF: a new mast-cell adhesion molecule used for attachment to fibroblasts and transcriptionally regulated by MITF Akihiko Ito, Tomoko Jippo, Tomohiko Wakayama, Eiichi Morii, Yu-ichiro Koma, Hiroaki Onda, Hiroshi Nojima, Shoichi Iseki, and Yukihiko Kitamura From the Department of Pathology, Osaka University Medical School/Graduate School of Frontier Bioscience, Suita, Osaka; the Department of Histology and Embryology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa; and the Department of Molecular Genetics, Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan. Microphthalmia transcription factor (MITF) is a basic-helix-loop-helix-leucine zipper-type transcription factor. The mutant mi and Miwh alleles encode MITFs with deletion and alteration of a single amino acid, respectively, whereas the tg is a null mutation. In coculture with NIH/3T3 fibroblasts, the numbers of cultured mast cells (CMCs) derived from C57BL/6 (B6)mi/mi, B6Miwh/Miwh, and B6tg/tg mice that adhered to NIH/3T3 fibroblasts were one third as large as the number of B6+/+ CMCs that adhered to NIH/3T3 fibroblasts. From a cDNA library of B6+/+ CMCs, we subtracted messenger RNAs expressed by B6mi/mi CMCs and found a clone encoding SgIGSF, a recently identified member of the immunoglobulin superfamily. Northern and Western blot analyses revealed that SgIGSF was expressed in B6+/+ CMCs but not in CMCs derived from MITF mutants. Immunocytochemical analysis showed that SgIGSF localized to the cell-to-cell contact areas between B6+/+ CMCs and NIH/3T3 fibroblasts. Transfection of B6mi/mi and B6tg/tg CMCs with SgIGSF cDNA normalized their adhesion to NIH/3T3 fibroblasts. NIH/3T3 fibroblasts did not express SgIGSF, indicating that SgIGSF acts as a heterophilic adhesion molecule. Transfection of B6tg/tg CMCs with normal MITF cDNA elevated their SgIGSF expression to normal levels. These results indicated that SgIGSF mediated the adhesion of CMCs to fibroblasts and that the transcription of SgIGSF was critically regulated by MITF. © 2003 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. J. Hasstedt, I. D. Bezemer, P. W. Callas, C. Y. Vossen, W. Trotman, R. P. Hebbel, C. Demers, F. R. Rosendaal, and E. G. Bovill Cell adhesion molecule 1: a novel risk factor for venous thrombosis Blood, October 1, 2009; 114(14): 3084 - 3091. [Abstract] [Full Text] [PDF] M. Hagiyama, N. Ichiyanagi, K. B. Kimura, Y. Murakami, and A. 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