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Prepublished online as a Blood First Edition Paper on November 21, 2002; DOI 10.1182/blood-2002-08-2438. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-08-2438v1 101/7/2693    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Asnafi, V. Articles by Macintyre, E. Search for Related Content PubMed PubMed Citation Articles by Asnafi, V. Articles by Macintyre, E. Related Collections Immunobiology Neoplasia Clinical Trials and Observations Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 April 2003, Vol. 101, No. 7, pp. 2693-2703 IMMUNOBIOLOGY Analysis of TCR, pT, and RAG-1 in T-acute lymphoblastic leukemias improves understanding of early human T-lymphoid lineage commitment Vahid Asnafi, Kheira Beldjord, Emmanuelle Boulanger, Béatrice Comba, Patricia Le Tutour, Marie-Hélène Estienne, Frédéric Davi, Judith Landman-Parker, Pierre Quartier, Agnès Buzyn, Eric Delabesse, Françoise Valensi, and Elizabeth Macintyre From the Department of Biological and Clinical Hematology, Centre Hospitalier-Universitaire/Assistance Publique-Hopitaux de Paris (CHU/AP-HP) Necker-Enfants Malades and Université Paris V; Hôpital Bretonneau, Tours; Hôpital La Pitié-Salpêtrière and Hôpital Trousseau, Paris, France. T-acute lymphoblastic leukemias (T-ALLs) derive from human T-lymphoid precursors arrested at various early stages of development. Correlation of phenotype and T-cell receptor (TCR) status with RAG-1 and pT transcription in 114 T-ALLs demonstrated that they largely reflect physiologic T-lymphoid development. Half the TCR lineage T-ALLs expressed a pre-TCR, as evidenced by RAG-1, pT, and cTCR expression, absence of TCR deletion, and a sCD3, CD1a+, CD4/8 double-positive (DP) phenotype, in keeping with a population undergoing  selection. Most TCR T-ALLs were pT, terminal deoxynucleotidyl transferase (TdT), and RAG-1lo/neg, double-negative/single-positive (DN/SP), and demonstrated only TCR DJ rearrangement, whereas 40% were pT, TdT, and RAG-1 positive, DP, and demonstrated TCR V(D)J rearrangement, with cTCR expression in proportion. As such they may correspond to TCR lineage precursors selected by TCR expression, to early cells recently derived from a pT+ common / precursor, or to a lineage-deregulated / intermediate. Approximately 30% of T-ALLs were sCD3/cTCR and corresponded to nonrestricted thymic precursors because they expressed non-T-restricted markers such as CD34, CD13, CD33, and CD56 and were predominantly DN, CD1a, pT, and RAG-1 low/negative, despite immature TCR and TCR rearrangements. TCR gene configuration identified progressive T-lymphoid restriction. T-ALLs, therefore, provide homogeneous expansions of minor human lymphoid precursor populations that can aid in the understanding of healthy human T-cell development. © 2003 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. Terriou, R. Ben Abdelali, C. Roumier, L. Lhermitte, J. de Vos, P. Cornillet, O. Nibourel, K. Beldjord, H. 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