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Prepublished online as a Blood First Edition Paper on January 9, 2003; DOI 10.1182/blood-2002-06-1866.
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Blood, 1 May 2003, Vol. 101, No. 9, pp. 3509-3513
IMMUNOBIOLOGY
Gene expression profile in human leukocytes
Shin-ichi Hashimoto,
Shigenori Nagai,
Jun Sese,
Takuji Suzuki,
Aya Obata,
Taku Sato,
Nobuaki Toyoda,
Hong-Yan Dong,
Makoto Kurachi,
Tomoyuki Nagahata,
Ken-ichi Shizuno,
Shinichi Morishita, and
Kouji Matsushima
From the Department of Molecular Preventive Medicine
and the Department of Complexity Science and Engineering, Graduate
School of Frontier Science, University of Tokyo,
Japan.
Leukocytes are classified as myelocytic or lymphocytic, and each
class of leukocytes consists of several types of cells that have
different phenotypes and different roles. To define the gene expression
in these cells, we have performed serial analysis of gene expression
(SAGE) using human leukocytes and have provided the gene database for
these cells not only at the resting stage but also at the activated
stage. A total of 709 990 tags from 17 libraries were analyzed for the
manifestation of gene expression profiles in various types of human
leukocytes. Types of leukocytes analyzed were as follows: peripheral
blood monocytes, colony-stimulating factor-induced macrophages,
monocyte-derived immature dendritic cells, mature/activated dendritic
cells, granulocytes, natural killer (NK) cells, resting B cells,
activated B cells, naive T cells, CCR4 memory T cells
(resting TH1 cells), CCR4+ memory T cells
(resting TH2 cells), activated TH1 cells, and activated TH2 cells. Among 38 961 distinct tags that
appeared more than once in the combined total libraries, 27 323 tags
were found to represent unique genes in certain type(s) of leukocytes. Using probability (P) and hierarchical clustering
analysis, we identified the genes selectively expressed in each type of
leukocytes. Identification of the genes specifically expressed in
different types of leukocytes provides not only a novel molecular
signature to define different subsets of resting and activated cells
but also contributes to further understanding of the biologic function of leukocytes in the host defense system.
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