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Blood, 1 December 2003, Vol. 102, No. 12, pp. 3989-3997. Prepublished online as a Blood First Edition Paper on August 14, 2003; DOI 10.1182/blood-2003-05-1540. This Article Full Text Full Text (PDF) Supplemental Data All Versions of this Article: 2003-05-1540v1 102/12/3989    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bottardi, S. Articles by Milot, E. Search for Related Content PubMed PubMed Citation Articles by Bottardi, S. Articles by Milot, E. Related Collections Gene Expression Hematopoiesis and Stem Cells Red Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  HEMATOPOIESIS Developmental stage–specific epigenetic control of human -globin gene expression is potentiated in hematopoietic progenitor cells prior to their transcriptional activation Stefania Bottardi, Angélique Aumont, Frank Grosveld, and Eric Milot From the Guy-Bernier Research Centre, Maisonneuve-Rosemont Hospital, Faculty of Medicine, University of Montreal, QC, Canada; and Department of Cell Biology and Genetics, Faculty of Medicine, Erasmus University, Rotterdam, The Netherlands. To study epigenetic regulation of the human -globin locus during hematopoiesis, we investigated patterns of histone modification and chromatin accessibility along this locus in hematopoietic progenitor cells (HPCs) derived from both humans and transgenic mice. We demonstrate that the developmentally related activation of human -like globin genes in humans and transgenic mice HPCs is preceded by a wave of gene-specific histone H3 hyperacetylation and K4 dimethylation. In erythroid cells, expression of -like globin genes is associated with histone hyperacetylation along these genes and, surprisingly, with local deacetylation at active promoters. We also show that endogenous mouse major and human -like genes are subject to different epigenetic control mechanisms in HPCs. This difference is likely due to intrinsic properties of the human -globin locus since, in transgenic mice, this locus is epigenetically regulated in the same manner as in human HPCs. Our results suggest that a defined pattern of histone H3 acetylation/dimethylation is important for specific activation of human globin promoters during development in human and transgenic HPCs. We propose that this transient acetylation/dimethylation is involved in gene-specific potentiation in HPCs (ie, before extensive chromatin remodeling and transcription take place in erythroid cells). CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. M. Coleman, V. Connor, Z. S. C. Cheng, F. Grey, C. M. Preston, and S. Efstathiou Histone modifications associated with herpes simplex virus type 1 genomes during quiescence and following ICP0-mediated de-repression J. Gen. Virol., January 1, 2008; 89(1): 68 - 77. [Abstract] [Full Text] [PDF] F. Zhang, S. I. Thornhill, S. J. Howe, M. Ulaganathan, A. Schambach, J. Sinclair, C. Kinnon, H. B. Gaspar, M. Antoniou, and A. J. Thrasher Lentiviral vectors containing an enhancer-less ubiquitously acting chromatin opening element (UCOE) provide highly reproducible and stable transgene expression in hematopoietic cells Blood, September 1, 2007; 110(5): 1448 - 1457. 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