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Blood, 15 December 2003, Vol. 102, No. 13, pp. 4431-4440. Prepublished online as a Blood First Edition Paper on August 7, 2003; DOI 10.1182/blood-2003-02-0500. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-02-0500v1 102/13/4431    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by de Saint-Vis, B. Articles by Garrone, P. Search for Related Content PubMed PubMed Citation Articles by de Saint-Vis, B. Articles by Garrone, P. Related Collections Hematopoiesis and Stem Cells Immunobiology Phagocytes Cell Adhesion and Motility Signal Transduction Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY Human dendritic cells express neuronal Eph receptor tyrosine kinases: role of EphA2 in regulating adhesion to fibronectin Blandine de Saint-Vis, Caroline Bouchet, Grégory Gautier, Jenny Valladeau, Christophe Caux, and Pierre Garrone From the Laboratory for Immunological Research, Schering-Plough, Dardilly, France; and Institut National de la Santé et de la Recherche Médicale (INSERM) U346, Hôpital E. Herriot, Pavillon R, Lyon, France. Eph receptor tyrosine kinases and their ligands, the ephrins, have been primarily described in the nervous system for their roles in axon guidance, development, and cell intermingling. Here we address whether Eph receptors may also regulate dendritic cell (DC) trafficking. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that DCs derived from CD34+ progenitors, but not from monocytes, expressed several receptors, in particular EphA2, EphA4, EphA7, EphB1, and EphB3 mRNA. EphB3 was specifically expressed by Langerhans cells, and EphA2 and EphA7 were expressed by both Langerhans- and interstitial-type DCs. EphA and EphB protein expression on DCs generated in vitro was confirmed by staining with ephrin-A3-Fc and ephrin-B3-Fc fusion proteins that bind to different Eph members, in particular EphA2 and EphB3. Immunostaining with anti-EphA2 antibodies demonstrated the expression of EphA2 by immature DCs and by skin Langerhans cells isolated ex vivo. Interestingly, ephrin expression was detected in epidermal keratinocytes and also in DCs. Adhesion of CD34+-derived DCs to fibronectin, but not to poly-L-lysine, was increased in the presence of ephrin-A3-Fc, a ligand of EphA2, through a 1 integrin activation pathway. As such, EphA2/ephrin-A3 interactions may play a role in the localization and network of Langerhans cells in the epithelium and in the regulation of their trafficking. (Blood. 2003;102:4431-4440) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Arvanitis and A. Davy Eph/ephrin signaling: networks Genes & Dev., February 15, 2008; 22(4): 416 - 429. [Abstract] [Full Text] [PDF] H. F. Langer, K. Daub, G. Braun, T. Schonberger, A. E. May, M. Schaller, G. M. Stein, K. Stellos, A. Bueltmann, D. Siegel-Axel, et al. Platelets Recruit Human Dendritic Cells Via Mac-1/JAM-C Interaction and Modulate Dendritic Cell Function In Vitro Arterioscler. Thromb. Vasc. Biol., June 1, 2007; 27(6): 1463 - 1470. [Abstract] [Full Text] [PDF] D. Pfaff, U. Fiedler, and H. G. Augustin Emerging roles of the Angiopoietin-Tie and the ephrin-Eph systems as regulators of cell trafficking J. Leukoc. 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