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Blood, 15 December 2003, Vol. 102, No. 13, pp. 4487-4492. Prepublished online as a Blood First Edition Paper on August 28, 2003; DOI 10.1182/blood-2003-07-2465. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-07-2465v1 102/13/4487    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Duramad, O. Articles by Barrat, F. J. Search for Related Content PubMed PubMed Citation Articles by Duramad, O. Articles by Barrat, F. J. Related Collections Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  IMMUNOBIOLOGY IL-10 regulates plasmacytoid dendritic cell response to CpG-containing immunostimulatory sequences Omar Duramad, Karen L. Fearon, Jean H. Chan, Holger Kanzler, Jason D. Marshall, Robert L. Coffman, and Franck J. Barrat From the Dynavax Technologies Corporation, Berkeley, CA. Immunostimulatory sequences (ISS) are short oligonucleotides containing unmethylated cytosine-phosphate-guanine (CpG) dinucleotides that stimulate innate immune responses through Toll-like receptor-9 on B cells and plasmacytoid dendritic cell (PDC) precursors. The anti-inflammatory cytokine interleukin (IL)-10 is predicted to be a potent inhibitor of many of the activities described for ISS, and this may impact the use of ISS in disease states characterized by elevated IL-10. As the activities of ISS on PDCs are central to many clinical applications of ISS, we have studied the effects of IL-10 on PDC stimulation by 3 distinct classes of ISS. IL-10 inhibited cytokine production and survival of ISS-activated PDCs; however, IL-12 induction was much more sensitive to inhibition than interferon (IFN)- induction. Within the PDC population are cells that respond to ISS by producing either IL-12 or IFN- but not both cytokines. IL-12-producing PDCs require costimulation through CD40 and appear more mature than IFN--producing PDCs. The 3 distinct classes of ISS differed with respect to induction of PDC maturation and T-cell priming capacity. IL-10 regulated PDC activation but did not inhibit the subsequent T-cell-priming ability of PDCs already activated by ISS. (Blood. 2003;102:4487-4492) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Sadaka, M.-A. Marloie-Provost, V. 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