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Blood, 15 December 2003, Vol. 102, No. 13, pp. 4600-4607. Prepublished online as a Blood First Edition Paper on August 21, 2003; DOI 10.1182/blood-2003-05-1428. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-05-1428v1 102/13/4600    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Poulin, J.-F. Articles by Cheynier, R. Search for Related Content PubMed PubMed Citation Articles by Poulin, J.-F. Articles by Cheynier, R. Related Collections Immunobiology Transplantation Apoptosis Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  TRANSPLANTATION Evidence for adequate thymic function but impaired naive T-cell survival following allogeneic hematopoietic stem cell transplantation in the absence of chronic graft-versus-host disease Jean-François Poulin, Myriam Sylvestre, Patrick Champagne, Marie-Lise Dion, Nadia Kettaf, Alain Dumont, Maryse Lainesse, Pierre Fontaine, Denis-Claude Roy, Claude Perreault, Rafick-Pierre Sékaly, and Rémi Cheynier From the Laboratoire d'Immunologie, Centre de Recherches du Centre Hospitalier de l'Université de Montréal (CHUM), Hôtel-Dieu, Montréal, QC; the Department of Medicine, Division of Experimental Medicine, and the Department of Microbiology and Immunology, McGill University, Montréal, QC; the Département de Microbiologie et Immunologie, Faculté de Médecine, Université de Montréal, QC; and the Département de Médecine, Université de Montréal and Centre de Recherche Hôpital Maisonneuve-Rosemont, Montréal, QC, Canada. Allogeneic hematopoietic stem cell transplantation (AHSCT) leads to a prolonged state of immunodeficiency characterized by low peripheral naive T-cell counts. To identify the mechanisms leading to this defect we quantitatively and qualitatively analyzed thymic function through quantification of T-cell receptor excision circle (TREC) frequencies (both the signal-joint TREC [sjTREC] and 6 different DJ TRECs, by-products of T-cell receptor [TCR] and gene rearrangement, respectively), in conjunction with immunophenotype and spectratype analyses in a cohort of patients sampled from 1 to 10 years following AHSCT. In this cohort, reduced thymic function was associated only with ongoing clinical chronic graft-versus-host disease (cGVHD). Nonetheless, the diversity of thymic production remained unchanged irrespective of the patient's cGVHD status. Interestingly, increased homeostatic proliferation was found in the naive T-cell compartment of cGVHD- patients who underwent transplantation. However, reduced expression of both the interleukin-7 receptor (IL-7R) (CD127) chain and the antiapoptotic protein Bcl-2 was observed. Taken together, these data indicate that the inability to reconstitute the naive T-cell compartment for several years after AHSCT, in the absence of cGVHD, is a consequence of impaired naive T-cell survival rather than thymic dysfunction. (Blood. 2003;102:4600-4607) CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M.-E. Blais, S. Brochu, M. Giroux, M.-P. Belanger, G. Dulude, R.-P. Sekaly, and C. Perreault Why T Cells of Thymic Versus Extrathymic Origin Are Functionally Different J. Immunol., February 15, 2008; 180(4): 2299 - 2312. [Abstract] [Full Text] [PDF] E. Castermans, F. Baron, E. Willems, N. Schaaf-Lafontaine, N. Meuris, A. Gothot, J.-F. Vanbellighen, C. Herens, L. Seidel, V. Geenen, et al. 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