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Prepublished online as a Blood First Edition Paper on March 27, 2003; DOI 10.1182/blood-2002-12-3834.
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Blood, 15 July 2003, Vol. 102, No. 2, pp. 421-428
PLENARY PAPERS
Common lymphoid progenitors rapidly engraft and protect against lethal murine cytomegalovirus infection after hematopoietic stem cell transplantation
Caroline Arber,
Andrew BitMansour,
Timothy E. Sparer,
John P. Higgins,
Edward S. Mocarski,
Irving L. Weissman,
Judith A. Shizuru, and
Janice M. Y. Brown
From the Department of Medicine, Divisions of Bone Marrow Transplantation and Infectious Diseases; Departments of Pathology and Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA.
Lymphoid deficiency after allogeneic hematopoietic cell transplantation (HCT) results in increased susceptibility to infection; however, transplantation of mature lymphocytes frequently results in a serious complication known as graft-versus-host disease (GVHD). Here we demonstrate in mice that both congenic as well as allogeneic transplantation of low numbers of highly purified common lymphoid progenitors (CLPs)—a rare population of lymphoid-lineage-committed bone marrow cells—accelerates immune reconstitution after lethal irradiation and rescue with hematopoietic stem cells (HSCs). After congenic transplantation, 3 x 103 CLPs protected against murine cytomegalovirus (MCMV) infection at a level roughly equivalent to 107 unfractionated lymph node cells. In the allogeneic model of matched unrelated donor HSC transplantation, cotransplantation of 3 x 103 CLPs protected thymus-bearing as well as thymectomized hosts from MCMV infection and attenuated disease severity. Immunohistochemistry in combination with antibody depletion of T and natural killer (NK) cells confirmed that CLP-derived as well as residual host lymphocytes contribute to antiviral protection. Importantly, transplantation of allogeneic CLPs provided a durable antiviral immunity without inducing GVHD. These data support the potential for composing grafts with committed progenitors to reduce susceptibility to viral infection following HCT.
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