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Prepublished online as a Blood First Edition Paper on March 20, 2003; DOI 10.1182/blood-2002-12-3853. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-12-3853v1 102/2/689    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dziarski, R. Articles by Gupta, D. Search for Related Content PubMed PubMed Citation Articles by Dziarski, R. Articles by Gupta, D. Related Collections Immunobiology Phagocytes Related Article in Blood Online Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 July 2003, Vol. 102, No. 2, pp. 689-697 PHAGOCYTES Defect in neutrophil killing and increased susceptibility to infection with nonpathogenic gram-positive bacteria in peptidoglycan recognition protein-S (PGRP-S)–deficient mice Roman Dziarski, Kenneth A. Platt, Eva Gelius, Håkan Steiner, and Dipika Gupta From the Northwest Center for Medical Education, Indiana University School of Medicine, Gary; Lexicon Genetics, The Woodlands, TX; and the Department of Microbiology, Stockholm University, Sweden Insect peptidoglycan recognition protein-S (PGRP-S), a member of a family of innate immunity pattern recognition molecules conserved from insects to mammals, recognizes bacterial cell wall peptidoglycan and activates 2 antimicrobial defense systems, prophenoloxidase cascade and antimicrobial peptides through Toll receptor. We show that mouse PGRP-S is present in neutrophil tertiary granules and that PGRP-S–deficient (PGRP-S-/-) mice have increased susceptibility to intraperitoneal infection with gram-positive bacteria of low pathogenicity but not with more pathogenic gram-positive or gram-negative bacteria. PGRP-S-/- mice have normal inflammatory responses and production of tumor necrosis factor (TNF-) and interleukin 6 (IL-6). Neutrophils from PGRP-S-/- mice have normal phagocytic uptake of bacteria but are defective in intracellular killing and digestion of relatively nonpathogenic gram-positive bacteria. Therefore, mammalian PGRP-S functions in intracellular killing of bacteria. Thus, only bacterial recognition by PGRP-S, but not its effector function, is conserved from insects to mammals. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Article in Blood Online: Peptidoglycan recognition and neutrophil function Jerrold Weiss Blood 2003 102: 419. [Full Text] [PDF] This article has been cited by other articles: P. C. F. Oyston, M. A. Fox, S. J. Richards, and G. C. Clark Novel peptide therapeutics for treatment of infections J. Med. Microbiol., August 1, 2009; 58(8): 977 - 987. [Abstract] [Full Text] [PDF] J. D. Lippolis Immunological signaling networks: Integrating the body's immune response J Anim Sci, April 1, 2008; 86(14_suppl): E53 - E63. 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