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Prepublished online as a Blood First Edition Paper on April 24, 2003; DOI 10.1182/blood-2002-11-3490. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-11-3490v1 102/4/1290    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Shelburne, C. P. Articles by Ryan, J. J. Search for Related Content PubMed PubMed Citation Articles by Shelburne, C. P. Articles by Ryan, J. J. Related Collections Transplantation Apoptosis Cell Cycle Signal Transduction Gene Expression Hematopoiesis Immunobiology Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 August 2003, Vol. 102, No. 4, pp. 1290-1297 HEMATOPOIESIS Stat5 expression is critical for mast cell development and survival Christopher P. Shelburne, Margaret E. McCoy, Roland Piekorz, Veronica Sexl, Kwan-Ho Roh, Sarah M. Jacobs-Helber, Sheila R. Gillespie, Daniel P. Bailey, Paria Mirmonsef, Meredith N. Mann, Mohit Kashyap, Harry V. Wright, Hey Jin Chong, L. Andrew Bouton, Brian Barnstein, Carlos D. Ramirez, Kevin D. Bunting, Steven Sawyer, Chris S. Lantz, and John J. Ryan From the Department of Biology, Virginia Commonwealth University, Richmond, VA; the Department of Biology, James Madison University, Harrisonburg, VA; the Department of Biochemistry, St Jude Children's Research Hospital, Memphis, TN; the Departments of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA; the Department of Hematopoiesis, Jerome H. Holland Laboratory for the Biomedical Sciences, Rockville, MD; and The George Washington University, Washington, DC. Interleukin-3 (IL-3) and stem cell factor (SCF) are important mast cell growth and differentiation factors. Since both cytokines activate the transcription factor signal transducer and activator of transcription 5 (Stat5), a known regulator of proliferation and survival, we investigated the effects of Stat5 deficiency on mast cell development and survival. Bone marrow–derived mast cell (BMMC) populations cultured from Stat5A/B-deficient mice survived in IL-3 + SCF, but not in either cytokine alone. These cells demonstrated reduced expression of Bcl-2, Bcl-x(L), cyclin A2, and cyclin B1, with increased apoptosis and delayed cell cycle progression during IL-3 or SCF culture. Finally, the absence of Stat5 resulted in loss of in vivo mast cell development, as judged by assessments of Stat5-deficient mice and transplantation of Stat5-deficient bone marrow cells to mast cell-deficient recipient mice. These results indicate that Stat5A and Stat5B are critical regulators of in vitro and in vivo mast cell development and survival. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Mayerhofer, K. V. Gleixner, A. Hoelbl, S. Florian, G. Hoermann, K. J. Aichberger, M. Bilban, H. Esterbauer, M.-T. Krauth, W. R. Sperr, et al. Unique Effects of KIT D816V in BaF3 Cells: Induction of Cluster Formation, Histamine Synthesis, and Early Mast Cell Differentiation Antigens J. 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