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Prepublished online as a Blood First Edition Paper on April 24, 2003; DOI 10.1182/blood-2002-08-2416. This Article Full Text Full Text (PDF) All Versions of this Article: 2002-08-2416v1 102/4/1381    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wong, S.-C. Articles by Lam, K.-P. Search for Related Content PubMed PubMed Citation Articles by Wong, S.-C. Articles by Lam, K.-P. Related Collections Immunobiology Signal Transduction Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 August 2003, Vol. 102, No. 4, pp. 1381-1388 IMMUNOBIOLOGY Impaired germinal center formation and recall T-cell–dependent immune responses in mice lacking the costimulatory ligand B7-H2 Siew-Cheng Wong, Edwin Oh, Chee-Hoe Ng, and Kong-Peng Lam From the Institute of Molecular and Cell Biology (IMCB), Singapore. B7-H2, which is expressed constitutively on B cells and binds the inducible costimulator (ICOS) on antigen-activated T cells, is a member of the B7 family of costimulatory ligands. We have inactivated B7-H2 in the mouse. B7-H2–/– mice generate normal populations of B and T cells in their various lymphoid organs but have lower basal levels of heavy chain class–switched antibodies in their sera. These mice are able to mount normal immune responses to both type I and type II T-cell–independent antigens. However, their pattern of responses to a T-cell–dependent antigen is altered, with greatly reduced production of antigen-specific heavy chain class–switched antibodies, the levels of which could not be elevated even with repeated immunizations. This suggests a critical role for B7-H2 in the recall phases of the immune response. Germinal center formation is also impaired in the mutant mice. While B cells from the mutant mice could response normally to anti-IgM, anti-CD40, and lipopolysaccharide stimulation, the production of T-helper–type II cytokines such as interleukin-4 (IL-4) and IL-10 by primed CD4+ T cells from mutant mice were reduced. This indicated that the defects in humoral responses and germinal center formation in B7-H2–deficient mice are due to the lack of T-cell–mediated help to the B cells. Hence, B7-H2 on B cells is important for recruiting T-cell help via its interaction with ICOS and plays a critical role in costimulating humoral immune responses. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Vu, U. Dianzani, C. F. Ware, T. Mak, and J. L. Gommerman ICOS, CD40, and Lymphotoxin {beta} Receptors Signal Sequentially and Interdependently to Initiate a Germinal Center Reaction J. Immunol., February 15, 2008; 180(4): 2284 - 2293. [Abstract] [Full Text] [PDF] Y. Burmeister, T. Lischke, A. C. Dahler, H. W. Mages, K.-P. Lam, A. J. Coyle, R. A. Kroczek, and A. Hutloff ICOS Controls the Pool Size of Effector-Memory and Regulatory T Cells J. Immunol., January 15, 2008; 180(2): 774 - 782. [Abstract] [Full Text] [PDF] A. H.-M. Tan, S. Y.-P. Goh, S.-C. Wong, and K.-P. Lam T Helper Cell-specific Regulation of Inducible Costimulator Expression via Distinct Mechanisms Mediated by T-bet and GATA-3 J. Biol. Chem., January 4, 2008; 283(1): 128 - 136. [Abstract] [Full Text] [PDF] T. Kallinich, K. C. Beier, U. Wahn, P. Stock, and E. 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[Abstract] [Full Text] [PDF] W.-I Lee, T. R. Torgerson, M. J. Schumacher, L. Yel, Q. Zhu, and H. D. Ochs Molecular analysis of a large cohort of patients with the hyper immunoglobulin M (IgM) syndrome Blood, March 1, 2005; 105(5): 1881 - 1890. [Abstract] [Full Text] [PDF] C. Hausl, R. U. Ahmad, H. P. Schwarz, E. M. Muchitsch, P. L. Turecek, F. Dorner, and B. M. Reipert Preventing restimulation of memory B cells in hemophilia A: a potential new strategy for the treatment of antibody-dependent immune disorders Blood, July 1, 2004; 104(1): 115 - 122. [Abstract] [Full Text] [PDF] W.-K. Suh, A. Tafuri, N. N. Berg-Brown, A. Shahinian, S. Plyte, G. S. Duncan, H. Okada, A. Wakeham, B. Odermatt, P. S. Ohashi, et al. The Inducible Costimulator Plays the Major Costimulatory Role in Humoral Immune Responses in the Absence of CD28 J. Immunol., May 15, 2004; 172(10): 5917 - 5923. [Abstract] [Full Text] [PDF]

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