|
|
Prepublished online as a Blood First Edition Paper on May 8, 2003; DOI 10.1182/blood-2002-11-3551.
 Previous Article | Table of Contents | Next Article 
Blood, 1 September 2003, Vol. 102, No. 5, pp. 1771-1778
IMMUNOBIOLOGY
Induction of FucT-VII by the Ras/MAP kinase cascade in Jurkat T cells
Sheila M. Barry,
Dimitrios G. Zisoulis,
Joel W. Neal,
Neil A. Clipstone, and
Geoffrey S. Kansas
From the Department of Microbiology-Immunology, Northwestern University
Medical School, Chicago, IL.
Induction of the  1,3-fucosyltransferase FucT-VII in T lymphocytes is
crucial for selectin ligand formation, but the signaling and transcriptional
pathways that govern FucT-VII expression are unknown. Here, using a novel,
highly phorbol myristate acetate (PMA)–responsive variant of the Jurkat
T-cell line, we identify Ras and downstream mitogen-activated protein (MAP)
kinase pathways as essential mediators of FucT-VII gene expression. PMA
induced FucT-VII in only a subset of treated cells, similar to expression of
FucT-VII in normal activated CD4 T cells. Introduction of constitutively
active Ras or Raf by recombinant retroviruses induced FucT-VII expression only
in that subset of cells expressing the highest levels of Ras, suggesting that
induction of FucT-VII required a critical threshhold of Ras signaling. Both
PMA treatment and introduction of active Ras led to rolling on E-selectin.
Pharmacologic inhibition studies confirmed the involvement of the classic
Ras-Raf-MEK–extracellular signal-regulated kinase (Ras-Raf-MEK-ERK)
pathway in FucT-VII induction by PMA, Ras, and Raf. These studies also
revealed a second, Ras-induced, Raf-1–independent pathway that
participated in induction of FucT-VII. Strong activation of Ras represents a
major pathway for induction of FucT-VII gene expression in T cells.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. Jennrich, B. A. Ratsch, A. Hamann, and U. Syrbe
Long-Term Commitment to Inflammation-Seeking Homing in CD4+ Effector Cells
J. Immunol.,
June 15, 2007;
178(12):
8073 - 8080.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G.-Y. Chen, H. Osada, L. F. Santamaria-Babi, and R. Kannagi
Interaction of GATA-3/T-bet transcription factors regulates expression of sialyl Lewis X homing receptors on Th1/Th2 lymphocytes
PNAS,
November 7, 2006;
103(45):
16894 - 16899.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Koelle, J. Huang, M. T. Hensel, and C. L. McClurkan
Innate Immune Responses to Herpes Simplex Virus Type 2 Influence Skin Homing Molecule Expression by Memory CD4+ Lymphocytes
J. Virol.,
March 15, 2006;
80(6):
2863 - 2872.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. H. Underhill, D. G. Zisoulis, K. P. Kolli, L. G. Ellies, J. D. Marth, and G. S. Kansas
A crucial role for T-bet in selectin ligand expression in T helper 1 (Th1) cells
Blood,
December 1, 2005;
106(12):
3867 - 3873.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Carlow, M. J. Williams, and H. J. Ziltener
Inducing P-Selectin Ligand Formation in CD8 T Cells: IL-2 and IL-12 Are Active In Vitro but Not Required In Vivo
J. Immunol.,
April 1, 2005;
174(7):
3959 - 3966.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. G. Zisoulis and G. S. Kansas
H-Ras and Phosphoinositide 3-Kinase Cooperate to Induce {alpha}(1,3)-Fucosyltransferase VII Expression in Jurkat T Cells
J. Biol. Chem.,
September 17, 2004;
279(38):
39495 - 39504.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
