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Prepublished online as a Blood First Edition Paper on May 1, 2003; DOI 10.1182/blood-2003-02-0347. This Article Full Text Full Text (PDF) All Versions of this Article: 2003-02-0347v1 102/5/1893    most recent Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chen, H. Articles by Vulpe, C. D. Search for Related Content PubMed PubMed Citation Articles by Chen, H. Articles by Vulpe, C. D. Related Collections Red Cells Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 September 2003, Vol. 102, No. 5, pp. 1893-1899 RED CELLS Systemic regulation of Hephaestin and Ireg1 revealed in studies of genetic and nutritional iron deficiency Huijun Chen, Trent Su, Zouhair K. Attieh, Tama C. Fox, Andrew T. McKie, Gregory J. Anderson, and Chris D. Vulpe From the Department of Nutritional Sciences and Toxicology, University of California, Berkeley; Department of Molecular Medicine, King's College, London, United Kingdom; and Joint Clinical Sciences Program, Queensland Institute of Medical Research and University of Queensland, Royal Brisbane Hospital, Brisbane, Queensland, Australia. Hephaestin is a membrane-bound multicopper ferroxidase necessary for iron egress from intestinal enterocytes into the circulation. Mice with sex-linked anemia (sla) have a mutant form of Hephaestin and a defect in intestinal basolateral iron transport, which results in iron deficiency and anemia. Ireg1 (SLC11A3, also known as Ferroportin1 or Mtp1) is the putative intestinal basolateral iron transporter. We compared iron levels and expression of genes involved in iron uptake and storage in sla mice and C57BL/6J mice fed iron-deficient, iron-overload, or control diets. Both iron-deficient wild-type mice and sla mice showed increased expression of Heph and Ireg1 mRNA, compared to controls, whereas only iron-deficient wild-type mice had increased expression of the brush border transporter Dmt1. Unlike iron-deficient mice, sla mouse enterocytes accumulated nonheme iron and ferritin. These results indicate that Dmt1 can be modulated by the enterocyte iron level, whereas Hephaestin and Ireg1 expression respond to systemic rather than local signals of iron status. Thus, the basolateral transport step appears to be the primary site at which the small intestine responds to alterations in body iron requirements. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Aydemir, S. Jenkitkasemwong, S. Gulec, and M. D. Knutson Iron Loading Increases Ferroportin Heterogeneous Nuclear RNA and mRNA Levels in Murine J774 Macrophages J. Nutr., March 1, 2009; 139(3): 434 - 438. [Abstract] [Full Text] [PDF] E. A. Milward, S. K. Baines, M. W. Knuiman, H. C. Bartholomew, M. L. Divitini, D. G. Ravine, D. G. Bruce, and J. K. Olynyk Noncitrus Fruits as Novel Dietary Environmental Modifiers of Iron Stores in People With or Without HFE Gene Mutations Mayo Clin. Proc., May 1, 2008; 83(5): 543 - 549. [Abstract] [Full Text] [PDF] C. Chicault, B. Toutain, A. Monnier, M. Aubry, P. Fergelot, A. L. Treut, M.-D. Galibert, and J. 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